Oral microbiota species in acute apical endodontic abscesses

Abstract

Background and objectives: Acute apical abscesses are serious endodontic diseases resulting from pulpal infection with opportunistic oral microorganisms. The objective of this study was to identify and compare the oral microbiota in patients (N 018) exhibiting acute apical abscesses, originating from the demographic region in Portland, Oregon. The study hypothesis is that abscesses obtained from this demographic region may contain unique microorganisms not identified in specimens from other regions. Design: Endodontic abscesses were sampled from patients at the Oregon Health & Science University (OHSU) School of Dentistry. DNA from abscess specimens was subjected to polymerase chain reaction amplification using 16S rRNA gene-specific primers and Cy3-dCTP labeling. Labeled DNA was then applied to microbial microarrays (280 species) generated by the Human Oral Microbial Identification Microarray Laboratory (Forsyth Institute, Cambridge, MA). Results: The most prevalent microorganisms, found across multiple abscess specimens, include Fusobacterium nucleatum, Parvimonas micra, Megasphaera species clone CS025, Prevotella multisaccharivorax, Atopobium rimae, and Porphyromonas endodontalis. The most abundant microorganisms, found in highest numbers within individual abscesses, include F. nucleatum, P. micra, Streptococcus Cluster III, Solobacterium moorei, Streptococcus constellatus, and Porphyromonas endodontalis. Strong bacterial associations were identified between Prevotella multisaccharivorax, Acidaminococcaceae species clone DM071, Megasphaera species clone CS025, Actinomyces species clone EP053, and Streptococcus cristatus (all with Spearman coefficients !0.9). Conclusions: Cultivable and uncultivable bacterial species have been identified in endodontic abscesses obtained from the Portland, Oregon demographic region, and taxa identifications correlated well with other published studies, with the exception of Treponema and Streptococcus cristae, which were not commonly identified in endodontic abscesses between the demographic region in Portland, Oregon and other regions.

Microbiota in Human Periodontal Abscess Revealed by 16S rDNA Sequencing

“…Furthermore, it was more abundant in periodontal abscess than in the pocket, suggesting that it is a potential, acute abscess-related, periodontal pathogen. A. rimae is an anaerobic, Gram-positive, rod-shaped bacterium, which has been suggested to be an endodontic abscess-related microorganism (Tennert et al, 2014; George et al, 2016). In the present study, A. rimae was found to be more significantly abundant in the abscess than in the pocket and healthy periodontium of the same patient.…”

Detection of Red complex bacteria, P. gingivalis, T. denticola and T. forsythia in infected root canals and their association with clinical signs and symptoms

“…Siqueira and Rocas analyzed five stages of experimental tools for analyzing microorganisms found within acute apical abscesses: 1) culture techniques; 2) molecular tools such as PCR and checkerboard hybridization assay; 3) PCR in addition to cloning and sequencing of targeted amplicons; 4) PCR or DNA hybridization including reverse checkerboard hybridization, and 5) next-generation sequencing. [ 31 32 ]…”

Inflammatory Networks Linking Oral Microbiome with Systemic Health and Disease

“…The periodontal pathogen P. gingivalis has been linked to cardiovascular diseases, lung disease, fetal loss, and rheumatoid arthritis, where local and systemic inflammation is suggested to act as the driving factor (Konkel et al 2019). F. nucleatum , in addition to its role in bridging biofilms and in oral diseases, has been implicated in a range of systemic diseases, including gastrointestinal abscesses (George et al 2016) and acute appendicitis (Swidsinski et al 2011), intra-amniotic infection (Gauthier et al 2011), colorectal cancers (Kelly et al 2018; Brennan and Garrett 2019; Komiya et al 2019), and pancreatic cancer (Mitsuhashi et al 2015; Gaiser et al 2019).…”