Oral Newcastle disease vaccination trials in Ethiopia

Abstract

Vaccination experiments were carried out in Ethiopia to study the efficacy of the NDV-I 2 vaccine against challenge with an Ethiopian velogenic strain of NDV. In experiment A, which comprised 300 broiler chicks, the efficacy of the ocular/drinking water application of the HB1/La Sota vaccine was compared with the ocular/drinking water and the feed application of the NDV-I 2 vaccine on untreated barley and sorghum. The NDV-I 2 vaccine applied by eye-drop or drinking-water protected the chickens against challenge as efficiently as combined HB1/La Sota vaccination but untreated barley and sorghum were unsuitable vaccine carriers. The vaccine virus could not be recovered and chickens neither seroconverted nor were they protected. In experiment B, 120 broiler chicks were divided into 6 treatment groups. One group each received NDV-I 2 vaccine mixed with untreated barley or sorghum which was applied immediately, or 14 h after mixing and standing at ambient temperature. The fifth group was vaccinated intraocularly and via the drinking water with the NDV-I 2 vaccine. The sixth group remained untreated. Experiment B confirmed the results of experiment A. In experiment C, 100 chicks were divided into 5 groups of 20 chickens each. One group each received the NDV-I 2 vaccine on parboiled barley or sorghum as vaccine carriers 0 and 6 h after mixing. The last group remained untreated. Parboiled barley given 0 or 6 h and parboiled sorghum given 0 h after mixing with the vaccine led to seroconversion and protection of the chickens. Parboiled sorghum given 6 h after mixing with the vaccine did not. It is concluded that the thermostable NDV-I 2 vaccine may be a suitable vaccine for oral application under Ethiopian conditions.

Evaluation of the newcastle disease antibody level after vaccination regimes in chickens in Debrezeit Agricultural Research Center, Ethiopia

“…Newcastle disease in commercial or village chickens is a problem through out the year although it is more serious at the beginning of the rainy season (Nassir, 1998). In many developing countries Newcastle disease (ND) appears to be the most important avian disease.…”

“…Poverty and protein deficiency is manifested by wide spread malnutrition in children and women in village communities (Nassir, 1998;Tadesse et al, 2005). Though neglected in the development themes for a long *Corresponding author.…”

Investigation of Newcastle Disease Virus Using Reverse Transcription Polymerase Chain Reaction in Selected Districts of Eastern Shewa, Ethiopia

“…Poultry production is hampered by wide arrays of constraints among which infectious diseases including Newcastle disease (ND) [6]. ND is one of the major problems in village chickens in most parts of Ethiopia and the disease has become endemic in poultry population and recurs every year inflicting heavy losses [7][8][9].…”

Effect of a velogenic newcastle disease virus on body and organ weights of vaccinated Shika brown cocks

“…These lesions are marke dly hemorrhagic and appear to result from necrosis on the intestinal wall or lymphoid foci such as cecal tonsils (Parede & Young, 1990). Suppression of immune response of the hosts may be due to temporary or permanent damage to primary lymphoid organs (Nasser et al, 2000). Generally gross lesions are not observed in the central nervous system of birds infected with NDV regardless of pathotype (McFerran & McCracken, 1988).…”