Oral Treatment of Chickens with Lactobacilli Influences Elicitation of Immune Responses

Brisbin, Jennifer T.; Gong, Joshua; Orouji, Shahriar; Esufali, Jessica; Mallick, Amirul Islam; Parvizi, Payvand; Shewen, Patricia E.; Sharif, Shayan

doi:10.1128/cvi.05100-11

Cited by 124 publications

(98 citation statements)

References 50 publications

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“…Evidence that dysbiosis may influence vaccine responses include direct effects of intestinal bacteria on the ability of polio virus to infect target cells in the intestine 11 and direct impairment by dysbiosis of the host immune response. 12 Studies showing that probiotic interventions increase immune responses to oral vaccines in adults 13 and animals 14 support this hypothesis, although results in infants and children are equivocal. 15,16 New DNA-based methods that use direct sequencing of short regions of the bacterial 16S ribosomal RNA (rRNA) gene produce a detailed picture of the gut microbiota, providing the relative abundance of taxa at multiple phylogenetic levels (ie, phylum, class, order, family, and genus).…”

mentioning

confidence: 67%

Stool Microbiota and Vaccine Responses of Infants

Huda

Lewis²,

Kalanetra³

et al. 2014

Pediatrics

337

331

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WHAT'S KNOWN ON THIS SUBJECT:Oral vaccine responses are low in children from less-developed countries perhaps as a result of intestinal dysbiosis. New high-throughput DNA-based methods allow characterization of intestinal microbiota as a predictor of vaccine responses.WHAT THIS STUDY ADDS: High abundance of stool Actinobacteria, including Bifidobacterium, was associated with higher responses to oral and parenteral vaccines and a larger thymus in Bangladeshi infants. Conversely, high abundance of Clostridiales, Enterobacteriales, and Pseudomonadales was associated with neutrophilia and lower vaccine responses. abstract OBJECTIVE: Oral vaccine efficacy is low in less-developed countries, perhaps due to intestinal dysbiosis. This study determined if stool microbiota composition predicted infant oral and parenteral vaccine responses. METHODS:The stool microbiota of 48 Bangladeshi infants was characterized at 6, 11, and 15 weeks of age by amplification and sequencing of the 16S ribosomal RNA gene V4 region and by Bifidobacterium-specific, quantitative polymerase chain reaction. Responses to oral polio virus (OPV), bacille Calmette-Guérin (BCG), tetanus toxoid (TT), and hepatitis B virus vaccines were measured at 15 weeks by using vaccine-specific T-cell proliferation for all vaccines, the delayed-type hypersensitivity skin-test response for BCG, and immunoglobulin G responses using the antibody in lymphocyte supernatant method for OPV, TT, and hepatitis B virus. Thymic index (TI) was measured by ultrasound.RESULTS: Actinobacteria (predominantly Bifidobacterium longum subspecies infantis) dominated the stool microbiota, with Proteobacteria and Bacteroidetes increasing by 15 weeks. Actinobacteria abundance was positively associated with T-cell responses to BCG, OPV, and TT; with the delayed-type hypersensitivity response; with immunoglobulin G responses; and with TI. B longum subspecies infantis correlated positively with TI and several vaccine responses. Bacterial diversity and abundance of Enterobacteriales, Pseudomonadales, and Clostridiales were associated with neutrophilia and lower vaccine responses.CONCLUSIONS: Bifidobacterium predominance may enhance thymic development and responses to both oral and parenteral vaccines early in infancy, whereas deviation from this pattern, resulting in greater bacterial diversity, may cause systemic inflammation (neutrophilia) and lower vaccine responses. Vaccine responsiveness may be improved by promoting intestinal bifidobacteria and minimizing dysbiosis early in infancy.

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mentioning

confidence: 67%

Stool Microbiota and Vaccine Responses of Infants

Huda

Lewis²,

Kalanetra³

et al. 2014

Pediatrics

337

331

View full text Add to dashboard Cite

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“…We evaluated the antigen-specific recall response by splenocytes cultured in the presence of antigen as described in previous studies [24,[29][30][31][32][33]. Because there were no detectable levels of the assayed cytokines in the supernatants of splenocytes cultured in the absence of antigen [24,29,30], in our study, lymphoid cells (4 × 10 5 cells per 200 μL) from the all groups of spleen were cultured with OVA (10 μg/mL) for 72 h at 37°C in RPMI-1640 medium (Sigma, USA) supplemented with 10% fetal calf serum (Hangzhou Sijiqing, China), 100 units/ml penicillin, and 100 μg/ml streptomycin (North China Pharmaceutical Corporation, China).…”

Section: In Vitro Cytokine Assays From Splenic Lymphoid Cells Restimumentioning

confidence: 99%

Intranasal administration of CpG oligodeoxynucleotides reduces lower airway inflammation in a murine model of combined allergic rhinitis and asthma syndrome

Zhang

Chen

et al. 2015

International Immunopharmacology

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“…Potencjalnie chorobotwórcze drobnoustroje zwiększają infiltrację tych komórek do błony podstawnej i warstwy nabłonka, czasami ze skutkiem pozytywnym, tzn. wygaszeniem zakażenia poprzez fagocytowanie patogenów i ich wewnątrzkomórkowe zabijanie (10,42,58). Niektóre bakterie, jak np.…”

Section: Znaczenie Mikrobiomu Jelitowegounclassified

Chicken intestinal microbiome: Development and function

Binek¹,

Cisek²,

Rzewuska³

et al. 2017

Medycyna Weterynaryjna

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SummaryChicken ceca contain an immense number of microorganisms collectively known as the microbiome. This community is now recognized as an essential component of the intestinal ecosystem and referred to as a metabolic organ exquisitely tuned to the host's physiology. These functions include the ability to process otherwise indigestible components of the feed, converting them into energy and body mass. The gut microbiome can also affect intestinal morphology and modulate the development and function of the immune system. This microbiota contains a rich collection of genes encoding enzymes necessary for decomposition of dietary polysaccharides and oligosaccharides, nitrogen metabolism, fatty acid and lipid metabolism, and pathways involved in a hydrogen sink. Chickens, like most animals, lack the genes for glycoside hydrolase, polysaccharide lyase, and carbohydrate esterase enzymes that are necessary to facilitate the degradation of non-starch polysaccharides. During the decomposition of dietary polysaccharides, bacteria produce short-chain (volatile) fatty acids (SCFAs), such as acetic, propionic and butyric acid. These SCFASs are absorbed transepithelially and serve as a source of energy for the host. The accumulation of molecular hydrogen released during fermentation leads to fermentation slowdown or to the production of less energy-efficient substances, such as ethanol, butyrate and propionate. The presence of bacteria that act as a hydrogen sink results in a switch to the more productive fermentation into acetate and increased production of SCFAs. Such activity could lead to a significant improvement in poultry production and the associated economics.

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Oral Treatment of Chickens with Lactobacilli Influences Elicitation of Immune Responses

Cited by 124 publications

References 50 publications

Stool Microbiota and Vaccine Responses of Infants

Stool Microbiota and Vaccine Responses of Infants

Intranasal administration of CpG oligodeoxynucleotides reduces lower airway inflammation in a murine model of combined allergic rhinitis and asthma syndrome

Chicken intestinal microbiome: Development and function

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