Oral versus intravenous administration of 5-aminolaevulinic acid for photodynamic therapy

Loh, C. S.; MacRobert, Alexander J.; Bedwell, J.; Reguła, Jarosław; Krasner, N; Bown, SG

doi:10.1038/bjc.1993.284

Cited by 244 publications

(121 citation statements)

References 20 publications

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“…administered ALA (unpublished data). Loh et al (1993b) found that the fluorescence kinetics after oral ALA administration was similar to that after i.v. administered ALA, although a higher ALA dose was necessary to achieve the same tissue concentrations of PpIX.…”

Section: Fluorescence Kinetic Studiesmentioning

confidence: 72%

“…With topical application an enhanced selective effect with PDT can be expected because of the restriction of the induced sensitiser to the lesion and the immediately surrounding normal skin. Loh et al (1993b) reported selective fluorescence of inoperable rectal adenocarcinomas after oral administration in two patients. Grant et al (1993) Figure 2a for group A and Figure 2b for Figure 2) after treatment for group B, but there was no complete circulation stop.…”

mentioning

confidence: 99%

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In vivo fluorescence kinetics and photodynamic therapy using 5-aminolaevulinic acid-induced porphyrin: increased damage after multiple irradiations

Veen¹,

Leengoed²,

Star³

1994

Br J Cancer

111

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The kinetics of fluorescence in tumour (TT) and subcutaneous tissue (ST) and the vascular effects of photodynamic therapy (PDT) were studied using protoporphyrin IX (PpIX), endogenously generated after i.v. administration of 100 and 200 mg kg-1 5-aminolaevulinic acid (ALA). The experimental model was a rat skinfold observation chamber containing a thin layer of ST in which a small syngeneic mammary tumour grows in a sheet-like fashion. Maximum TT and ST fluorescence following 200 mg kg-1 ALA was twice the value after 100 mg kg-1 ALA, but the initial increase with time was the same for the two doses in both TT and ST. The fluorescence increase in ST was slower and the maximum fluorescence was less and appeared later than in TT. Photodynamic therapy was applied using green argon laser light (514.5 nm, 100 J cm-2). Three groups received a single light treatment at different intervals after administration of 100 or 200 mg kg-1 ALA. In these groups no correlation was found between the fluorescence intensities and the vascular damage following PDT. A fourth group was treated twice and before the second light treatment some fluorescence had reappeared after photobleaching due to the first treatment. Only with the double light treatment was lasting TT necrosis achieved, and for the first time with any photosensitiser in this model this was accomplished without complete ST necrosis. Images Figure 4

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Section: Fluorescence Kinetic Studiesmentioning

confidence: 72%

mentioning

confidence: 99%

In vivo fluorescence kinetics and photodynamic therapy using 5-aminolaevulinic acid-induced porphyrin: increased damage after multiple irradiations

Veen¹,

Leengoed²,

Star³

1994

Br J Cancer

111

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“…Other approaches for achieving better localisation include local administration of the photosensitiser (Amano et al, 1988;Bachor et al, 1992). Recently, there has been considerable interest in a different approach to PDT in which a precursor, ALA, is administered and synthesis of the photosensitiser, PpIX, accomplished in situ (Pottier et al, 1986;Malik & Lugaci, 1987;Malik et al, 1989;Divaris et al, 1990;Kennedy et al, 1990;Bedwell et al, 1992;Kennedy & Pottier, 1992;Loh et al, 1992Loh et al, , 1993aRebeiz et al, 1992). The synthesis of ALA is the rate-limiting step in non-erythroid cells in the pathway of haem biosynthesis (Martin, 1985) (Rebeiz et al, 1992).…”

mentioning

confidence: 99%

A mechanistic study of cellular photodestruction with 5-aminolaevulinic acid-induced porphyrin

Iinuma

Farshi²,

Ortel³

et al. 1994

Br J Cancer

237

122

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“…This can be overcome by endogenous photosensitization with oral or intravenous administration of ALA. It was shown that oral ALA administration takes more time than intravenous application to induce PPIX fluorescence in the various tissues, but shows fewer side effects (Van den Boogert et al, 1985;Loh et al, 1993). Oral application of ALA is thus accompanied by systemic photosensitization, but in contrast to other porphyrins, which stay within the body for many days or weeks, photosensitization by ALA is limited to 24 hours after intake.…”

Section: Discussionmentioning

confidence: 99%

Photodynamic diagnosis of breast tumours after oral application of aminolevulinic acid

Ladner

Steiner²,

Allemann³

et al. 2001

Br J Cancer

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Photodynamic diagnosis is of increasing interest for diagnosis in oncology. It is based on a more intense incorporation of a fluorescent dye in tumours compared to normal tissue. As a feasibility study we investigated the effectiveness of oral application of 5-aminolevulinic acid for photodynamic diagnosis of human primary mammary tumours. The study included 16 patients with palpable breast tumours. Aminolevulinic acid was administered at a concentration of 40 mg kg −1 bodyweight 150–420 min prior to tumourectomy. Intraoperatively blue light (405 nm) was applied to the operation site. Sections of the excised tumour and some lymph nodes were prepared and analysed with a fluorescent microscope. All primary mammary tumour tissues showed significantly higher fluorescence intensity than surrounding normal mammary tissue. Fluorescence of the mammary tumours could also be discriminated macroscopically and intraoperatively. Fluorescence intensity in nonmetastatic lymph node tissue was higher in 2 out of 3 patients than in primary tumour tissue. By photodynamic diagnosis using aminolevulinic acid we were able to reliably distinguish primary mammary tumours from normal mammary tissue microscopically and macroscopically in all our patients. We suggest that photodynamic diagnosis with aminolevulinic acid for breast tumours should be further investigated and developed for intraoperative use and may well be a simple tool for better intraoperative diagnosis and recognition of tumour margins. We hypothesize that lymph node metastasis of breast tumours will not be detectable by this method. © 2001 Cancer Research Campaign http://www.bjcancer.com

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Oral versus intravenous administration of 5-aminolaevulinic acid for photodynamic therapy

Cited by 244 publications

References 20 publications

In vivo fluorescence kinetics and photodynamic therapy using 5-aminolaevulinic acid-induced porphyrin: increased damage after multiple irradiations

In vivo fluorescence kinetics and photodynamic therapy using 5-aminolaevulinic acid-induced porphyrin: increased damage after multiple irradiations

A mechanistic study of cellular photodestruction with 5-aminolaevulinic acid-induced porphyrin

Photodynamic diagnosis of breast tumours after oral application of aminolevulinic acid

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