Oral vitamin C attenuates acute ischaemia-reperfusion injury in skeletal muscle

Abstract

I schaemia-reperfusion injury (IRI) is caused by endothelial and subendothelial damage by neutrophil-derived oxidants. Vitamin C is an antioxidant which attenuates endothelial injury after IRI. Our aim was to evaluate the effect of oral vitamin C in the prevention of IRI in skeletal muscle. We used a model of cross-clamping (3 hours) and reperfusion (1 hour) of the cremaster muscle in rats. Muscle function was assessed electrophysiologically by electrical field stimulation. Infiltration by neutrophils was determined by the activity of tissue myeloperoxidase (MPO) and tissue oedema by the wet-to-dry ratio. Neutrophil respiratory burst activity was measured in control animals and groups pretreated with vitamin C.IRI significantly decreased muscle function and increased muscle neutrophil MPO activity and muscle oedema. Pretreatment with vitamin C preserved muscle function and reduced tissue oedema and neutrophil infiltration. Neutrophil respiratory burst activity was reduced in the group treated with vitamin C compared with the control group.We conclude that pretreatment with oral vitamin C protects against acute muscle IRI, possibly by attenuating neutrophil respiratory burst activity.

Hypertonic saline reduces skeletal muscle injury and associated remote organ injury following ischemia reperfusion injury

“…Restoration of blood flow to an acutely ischemic limb initiates a cascade of cellular and biochemical events that result in muscle edema, necrosis, and impaired muscle function (Kearns et al 2001). The associated lung injury is mediated by the adherence of activated neutrophils to the endothelium of the pulmonary microvasculature, resulting in spillage of protein-rich transudate into the alveolar spaces-hallmarks of the acute respiratory distress syndrome (ARDS) (Barry et al 1997).…”

“…Much of the previous work on skeletal muscle ischemia reperfusion injury has focused on the effects of pretreatment with various agents (Kearns et al 2001). While this is important to further our understanding of this complex process and to elucidate the underlying mechanisms involved, this does not always correlate with the clinical setting where ischemia is often a sudden unpredictable event.…”

The Effects of Iloprost and Vitamin C on Kidney as a Remote Organ After Ischemia/Reperfusion of Lower Extremities

“…Kearns et al [15] found that after 30 min infrarenal aortic cross-clamping and 120 min reperfusion in I/R injury protects against I/R-induced acute lung injury by vitamin C therapy. Still, Kearns et al [29] documented that pretreatment with oral vitamin C relieves endothelial injury against acute muscle I/R injury as an antioxidant agent possibly by attenuating neutrophil respiratory burst activity. Herbaczynska-Cedro et al [30] demonstrated that vitamin C reduces oxygen free radicals production in human circulating neutrophils.…”

Ascorbic acid prolongs the viability and stability of isolated perfused lungs: A mechanistic study using 31P and hyperpolarized 13C nuclear magnetic resonance

“…Amongst all antioxidants, ascorbate appears to be the most attractive option for treating and preventing IRI in the lung [20,21] and other organs [24][25][26][27][28][29]. Ascorbate administration has been shown to reduce oxidative damage in endothelial cells [30,31], improve tissue perfusion [32], inhibit tumor necrosis factor α (TNFα) [33], reduce mitochondrial swelling and damage [27], preserve mitochondrial respiration [34], reduce edema [35], reduce proinflammatory chemokine expression [36], and reduce oxidative damage [37,38].…”