Orally Administered Lactoperoxidase Increases Expression of the FK506 Binding Protein 5 Gene in Epithelial Cells of the Small Intestine of Mice: A DNA Microarray Study

Orally administered lactoperoxidase ameliorates dextran sulfate sodium-induced colitis in mice by up-regulating colonic interleukin-10 and maintaining peripheral regulatory T cells

“…Subcutaneously injected LPO reduces efferent lymphocyte output and lymph flow rate in the local lymph nodes of sheep [20]. We found orally administered LPO suppressed symptoms of pneumonia in mice infected with the influenza virus through inhibition of inflammatory cells in the lung and pro-inflammatory cytokines [21]; LPO also upregulated genes inducible by glucocorticoid, a steroidal hormone with anti-inflammatory activity, in the small intestine of mice [22]. More recently, we reported orally administered LPO ameliorates dextran sulfate sodium (DSS)-induced colitis in mice [23], an animal model with symptoms similar to UC in humans [24].…”

“…Orally administered LPO may help to selectively develop Treg from naive T cells to maintain the pool of Treg in the inflammatory status. We previously reported that orally administered LPO up-regulates several genes known to be induced by glucocorticoid, a steroidal hormone with antiinflammatory activity, in the small intestine of mice [22]. Administration of glucocorticoid increased the gene expression of IL-10 and Foxp3 in CD4 + T cells and the frequency of Tregs in asthma patients [37].…”

“…LPO purified from bovine milk was purchased from Biopole (Gembloux, Belgium) and further purified as described previously [22]. The freeze-dried, purified LPO powder had a purity index (A 412 /A 280 ) of 0.91 and a purity of 96.0% as determined by high performance liquid chromatography.…”

Inhibitory effect of lactoperoxidase on the secretion of proinflammatory cytokine interleukin-8 in human intestinal epithelial Caco-2 cells

“…Oral administration of LPO showed antiviral activity toward the influenza virus (Shin et al, 2005) and also an anti-inflammatory effect on the dextran sulfate sodium (DSS)-induced inflammation in a UC model mouse (Horigome, 2007), suggesting that LPO retains its active form in the intestinal tract. Wakabayashi et al (2007) using a DNA microarray analysis have recently found that oral administration of LPO in mice would modulate the intestinal immune system, because several immunity-related genes, such as the FKBP-5 (FK506-binding protein-5) gene, in the intestinal epithelial cells were up-regulated by LPO administration. It is known that the functions of FK506 and certain FKBPs are closely connected with the redox state of the cellular proteins (Klettner et al, 2001;Zissimopoulos, Docrat, & Lai, 2007).…”

Lactoperoxidase Immobilized Onto Various Beads For Producing Natural Preservatives Solution

“…LPO is a member of the peroxidase family and widely distributed in plants, animals, and human (Kussendrager and Hooijdonk, 2000). Hypothiocyanite produced by LPOs has been shown to be effective against pathogenic bacteria (Borch et al, 1989) as well as non--pathogenic bacteria (Wolfson and Sumner, 1993, Fonteh et al, 2005, Wakabayashi et al, 2007. Immobilized LPO is a very useful method to efficiently produce hypothiocyanite as a preservatives (Al--Baarri et al, 2010, Al--Baarri et al, 2011.…”