Orally Administered Liposomal Lactoferrin Inhibits Inflammation‐Related Bone Breakdown Without Interrupting Orthodontic Tooth Movement

Kawazoe, Aki; Inubushi, Toshihiro; Miyauchi, Mutsumi; Ishikado, Atsushi; Tanaka, Eiji; Tanne, Kazuo; Takata, Takashi

doi:10.1902/jop.2012.120508

Cited by 20 publications

(9 citation statements)

References 31 publications

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“…A previous study has shown that chronic alcohol intoxication decreases salivary lactoferrin output (Waszkiewicz et al, 2012). Though it has been demonstrated that orally administered liposomal lactoferrin inhibits LPS-induced alveolar bone resorption (Kawazoe et al, 2012), until now there are no direct reports on lactoferrin changes in the saliva after LPS infection as far as we know. This study firstly demonstrates that the salivary lactoferrin content decreases after LPS challenge.…”

Section: Discussionmentioning

confidence: 94%

Response to lipopolysaccharide in salivary components and the submandibular gland of pigs

et al. 2014

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Section: Discussionmentioning

confidence: 94%

Response to lipopolysaccharide in salivary components and the submandibular gland of pigs

et al. 2014

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“…The results of the present study demonstrate that LbLF inhibits the LPS-induced upregulation of TNF-α mRNA expression. In our previous study, it was revealed that orally administered LbLF significantly inhibits LPS-induced alveolar bone resorption ( 28 ). We also previously demonstrated the anti-inflammatory effect of LF ( 29 ), and other studies have observed that LF may interact with epithelial and immune cells in the intestinal mucosa ( 30 , 31 ).…”

Section: Discussionmentioning

confidence: 99%

Inhibition of DMH‑DSS‑induced colorectal cancer by liposomal bovine lactoferrin in rats

et al. 2017

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Abstract. Bovine lactoferrin (bLF) is a multifunctional protein with anti-inflammatory, antibacterial, antiviral, anti-tumour and immunoregulatory effects. The present study was conducted to evaluate the anti-inflammatory and anti-tumour effects of liposomal bLF (LbLF) in a 1,2-dimethylhydrazine (DMH)/dextran sulphate sodium (DSS)-induced model of carcinogenesis in F344 rats. F344 rats were randomly divided into three groups: Control (water), 500 or 1,000 mg/kg/day LbLF; additionally, the rats were injected with DMH (20 mg/kg) once per week for 8 consecutive weeks, after one week of drinking water containing 1% DSS. All rats were sacrificed at 25 weeks. The tissues were examined for the presence of aberrant crypt foci (ACF) and subjected to histopathological analysis. Additionally, human colon cancer cells were utilised to investigate the effect of LbLF on proliferation and inflammation. Rats from the 500 and 1,000 mg/kg/day LbLF groups harboured significantly fewer colon ACF, adenomas and adenocarcinomas than the rats from the control group. Lastly, it was demonstrated that LbLF inhibits cell growth and TNF-α mRNA expression. These data support the hypothesis that LbLF affects colorectal carcinogenesis by suppressing inflammation and cell proliferation in rats.

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“…Advantages of using mice as a model include the considerable background information on their biological system, a wide range of genetically engineered strains (e.g., gene knockouts for key receptors or signaling molecules) and availability of high‐quality chemical reagents . Although numerous animal studies have been carried out to investigate OF on periodontal cellular responses and bone loss under physiological conditions , only a few animal studies involving orthodontic treatment in the context of periodontal disease were performed, and most of them were performed in rats but not mice , presumably due to the technical challenges having to do with the relatively small size of the teeth and oral cavity of the mouse. To our knowledge, this is the first time a mouse model was established to investigate the potential mechanism of OF‐exacerbated PBL in experimental periodontitis.…”

Section: Discussionmentioning

confidence: 99%

Antibiotic administration alleviates the aggravating effect of orthodontic force on ligature‐induced experimental periodontitis bone loss in mice

Shi

Liu

Kawai³

et al. 2017

J of Periodontal Research

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Background and objectives It is recognized that orthodontic force has an aggravating effect on the progression of destructive periodontitis if periodontitis have not been well controlled. However, the underlying mechanism is not completely clear. This study was to investigate the effect of antibiotic administration on orthodontic force-aggravated, ligature-induced experimental periodontitis in mice. Methods C57BL/6 mice (male, 8-week old) were divided into three groups (n=8). Silk ligatures (SL) were tied around the maxillary right (Group 1) or both (Groups 2&3) first molars on Day 0, removed on Day 8, and systemic antibiotics (SA) was administered through drinking water (Group 3) since Day 8. Orthodontic force (OF) was applied on the maxillary right first molars since Day 13 (Groups 2&3). All mice were sacrificed on Day 20. Results Total oral bacteria load was significantly higher in Group 2 when compared to Group 1 on Day 20, whereas such count was greatly reduced in Group 3 when antibiotics were administered. Periodontal bone loss (PBL) was significantly increased on SL side vs. control side in Group 1. PBL was significantly increased on OF+SL side vs. SL side in Group 2 (p < 0.05) but not in Group 3 when SA was administered. Gingival mRNA and protein expressions of receptor activator of nuclear factor kappa-B ligand (RANKL) / osteoprotegerin (OPG) were significantly increased on OF+SL side vs. SL side in Group 2 (p < 0.01) but not in Group 3. However, comparable levels of TRAP+ cell formation within periodontal space and tooth movement were observed on OF+SL side in Group 2 and Group 3. Conclusion Our results suggest that reduction of oral bacterial load by antibiotic administration alleviate orthodontic force-aggravated periodontitis bone loss.

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Orally Administered Liposomal Lactoferrin Inhibits Inflammation‐Related Bone Breakdown Without Interrupting Orthodontic Tooth Movement

Abstract: Orally administered LbLF significantly inhibits LPS-induced alveolar bone resorption but not OF-induced bone remodeling. LbLF could be a potent therapeutic and preventive agent to control periodontal inflammation in patients undergoing orthodontic treatment.

Cited by 20 publications

References 31 publications

Response to lipopolysaccharide in salivary components and the submandibular gland of pigs

Response to lipopolysaccharide in salivary components and the submandibular gland of pigs

Inhibition of DMH‑DSS‑induced colorectal cancer by liposomal bovine lactoferrin in rats

Antibiotic administration alleviates the aggravating effect of orthodontic force on ligature‐induced experimental periodontitis bone loss in mice

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