Orally administered multispecies probiotic formulations to prevent uro-genital infections: a randomized placebo-controlled pilot study

Mezzasalma, Valerio; Manfrini, Enrico; Ferri, Emanuele; Boccarusso, Marco; Gennaro, Patrizia Di; Schiano, Irene; Michelotti, Angela; Labra, Massimo

doi:10.1007/s00404-016-4235-2

Cited by 30 publications

(20 citation statements)

References 27 publications

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“…Although oral probiotics can prevent and/or decrease frequency or severity of diseases outside of the gastrointestinal tract (i.e., an indirect effect) (Alberda et al, 2007; Lappin et al, 2009; Di Nardo et al, 2014; Esposito et al, 2014; Lee et al, 2015; Zuccotti et al, 2015; Fuchs-Tarlovsky et al, 2016; Gruner et al, 2016; Vieira et al, 2016; Zamani et al, 2016), few studies have directly evaluated the appearance of orally administered probiotic species or changes in host microbial communities at distant sites (De Alberti et al, 2015; Mastromarino et al, 2015; Treven et al, 2015). A recent study in pre-menopausal women demonstrated an increase in orally administered probiotic species in vaginal swabs using qPCR analysis, setting the stage for use of oral probiotics to prevent or treat urogenital infections (Mezzasalma et al, 2017). A combination of bovine lactoferrin and two probiotics administered to healthy women led to detection of the probiotic species in vaginal swabs, important pilot data for future studies of bacterial vaginosis (De Alberti et al, 2015).…”

Section: Discussionmentioning

confidence: 99%

Oral Probiotics Alter Healthy Feline Respiratory Microbiota

et al. 2017

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Probiotics have been advocated as a novel therapeutic approach to respiratory disease, but knowledge of how oral administration of probiotics influences the respiratory microbiota is needed. Using 16S rRNA amplicon sequencing of bacterial DNA our objective was to determine whether oral probiotics changed the composition of the upper and lower airway, rectal, and blood microbiota. We hypothesized that oral probiotics would modulate the respiratory microbiota in healthy cats, demonstrated by the detection and/or increased relative abundance of the probiotic bacterial species and altered composition of the microbial population in the respiratory tract. Six healthy young research cats had oropharyngeal (OP), bronchoalveolar lavage fluid (BALF), rectal, and blood samples collected at baseline and 4 weeks after receiving oral probiotics. 16S rRNA gene amplicon libraries were sequenced, and coverage, richness, and relative abundance of representative operational taxonomic units (OTUs) were determined. Hierarchical and principal component analyses (PCA) demonstrated relatedness of samples. Mean microbial richness significantly increased only in the upper and lower airways. The number of probiotic OTUs (out of 5 total) that significantly increased in relative abundance vs. baseline was 5 in OP, 3 in BAL and 2 in feces. Using hierarchical clustering, BALF and blood samples grouped together after probiotic administration, and PERMANOVA supported that these two sites underwent significant changes in microbial composition. PERMANOVA revealed that OP and rectal samples had microbial population compositions that did not significantly change. These findings were visualized via PCA, which revealed distinct microbiomes in each site; samples clustered more tightly at baseline and had more variation after probiotic administration. This is the first study describing the effect of oral probiotics on the respiratory microbiota via detection of probiotic species in the airways. Finding bacterial species present in the oral probiotics in the upper and lower airways provides pilot data suggesting that oral probiotics could serve as a tool to target dysbiosis occurring in inflammatory airway diseases such as feline asthma, a disease in which cats serve as an important comparative and translational model for humans.

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Section: Discussionmentioning

confidence: 99%

Oral Probiotics Alter Healthy Feline Respiratory Microbiota

et al. 2017

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“…In a previous study, we have demonstrated that our oral food supplement Intimique Femme is effective in colonizing the vaginal epithelium and that both the single strains and their combination can inhibit a wide panel of harmful pathogens, responsible for several vaginal infections [20].…”

Section: Discussionmentioning

confidence: 99%

Evaluation of an Orally Administered Multistrain Probiotic Supplement in Reducing Recurrences Rate of Bacterial Vaginosis: A Clinical and Microbiological Study

Murina¹,

Vicariotto²

2019

AID

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Background: Bacterial vaginosis (BV) is the most common urogenital disease in women, affecting about 19%-24% of them in reproductive ages annually and after treatment, a single recurrence or more may occur in up to 58% of women within 12 months. Objective: The aim was to evaluate the effectiveness of a new orally administered food supplement, containing different probiotic strains, on women of childbearing age after the antibiotic treatment when compared with no probiotic intake. Methods: A prospective study was undertaken on 62 patients with BV. All patients were cured with metronidazole vaginal formulations (5 g of 0.75% gel once daily for 5 days or 500 mg ovules once daily for 7 days), then after was offered the option of using a new an orally administered food supplement containing: Lactobacillus plantarum PBS067, Lactobacillus rhamnosus LRH020 and Bifidobacterium animalis lactis BL050, with a total viability of 3 × 10 9 CFU/capsule (Intimique ® Femme). Among these women, 50 accepted to use the new orally food supplement, while 25 patients decided to use only metronidazole (control group). Results: The recurrence rate of BV after treatment with Intimique ® Femme was about 16%, compared to 40% in the control group. The incidence of abnormal vaginal microbiota decreased in both groups, but it was significantly higher in the Intimique ® Femme group at the end of treatment. Conclusion: This study showed that, in case of BV diagnosis, the complementary treatment of a strain-specific probiotic complex after antibiotics prophylaxis, is mandatory to reduce potential recurrences and cyclic use of further antibiotics.

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“…Furthermore, the strains Lactobacillus rhamnosus HS111 and Lactobacillus acidophillus HS101 were also probiotic species effective in the treatment of candidiasis of complete denture wearers [ 15 ]. Not only in the dental area, but also in gynecology, there is interest in the subject [ 22 – 24 ] since the uncontrolled growth of Candida species is also responsible for gynecological infections. However, studies employing the pathogenic Lactobacillus species in the treatment of Candida infections are scarce [ 25 – 27 ].…”

Section: Introductionmentioning

confidence: 99%

Influence of the use of complete denture adhesives on microbial adhesion and biofilm formation by single- and mixed-species

et al. 2018

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ObjectivesTo verify whether the Ultra Corega Cream and Corega Strip Denture Adhesive adhesives interfere in the microbial adhesion and biofilm formation by Candida albicans and Lactobacillus casei in single- and mixed-species settings, and observe whether synergistic or antagonistic relationships between these species occur.MethodsSpecimens made from heat-polymerized acrylic resin (Lucitone 550) were fabricated (n = 144) with a circular shape and standardized roughness (3.0 μm ±0.3 Ra) and were divided into three groups: Without Adhesive (WA), with Ultra Corega Cream adhesive (CA) and Corega Strips adhesive (SA). These groups were divided into three subgroups each: C. albicans single-species, L. casei single-species and C. albicans with L. casei (mixed-species). Microbial adhesion and biofilm formation assays were performed in duplicate at four distinct experimental times (n = 8 per experimental condition). The amount of each microorganism on the surfaces of the specimens was observed by counting of the Colony Forming Units (CFU) per substrate. Additional specimens were characterized by Scanning Electron Microscopy (SEM), with 18 specimens being used in this analysis (n = 18), 2 per experimental condition (n = 2). Two-way ANOVA and Tukey’s test for multiple comparisons were employed, using α≤0.05.ResultsL. casei (mixed-species) adhered more on the WA substrate than the CA, while C. albicans (single- and mixed-species) adhered more on the SA. C. albicans, both single- and mixed-species adhered more than the L. casei (single- and mixed-species), regardless of the substrate. L. casei (single-species) formed more biofilm on the WA, but in its mixed cultivation, it had no difference of growth among the tested situations. C. albicans (single- and mixed-species) formed more biofilm on the SA than the CA, and the fungus formed more biofilm when compared to L. casei. In general, whenever a species was compared in its single- and mixed-species situation, no statistically significant difference was observed. SEM of biofilm formation assays demonstrated that L. casei single-species WA formed more biofilm than when the adhesives tested were used, and C. albicans (both single- and mixed-species) formed more biofilm on the SA than on the CA.Conclusions(1) The two denture adhesives tested increased the adhesion of C. albicans but not of L. casei; (2) biofilm formation by C. albicans (single- and mixed-species) was increased on the SA; (3) Relations of synergism or antagonism was not observed between the two microorganisms studied.

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Orally administered multispecies probiotic formulations to prevent uro-genital infections: a randomized placebo-controlled pilot study

Cited by 30 publications

References 27 publications

Oral Probiotics Alter Healthy Feline Respiratory Microbiota

Oral Probiotics Alter Healthy Feline Respiratory Microbiota

Evaluation of an Orally Administered Multistrain Probiotic Supplement in Reducing Recurrences Rate of Bacterial Vaginosis: A Clinical and Microbiological Study

Influence of the use of complete denture adhesives on microbial adhesion and biofilm formation by single- and mixed-species

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