Orally delivered dsRNA induces knockdown of target genes and mortality in the Asian long‐horned beetle, <i>Anoplophora glabripennis</i>

Dhandapani, Ramesh Kumar; Duan, Jian J.; Palli, Subba Reddy

doi:10.1002/arch.21679

Cited by 18 publications

(14 citation statements)

References 22 publications

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“…The only cerambycid to be examined for dietary RNAi susceptibility is Asain long-horned beetle ( Anoplophora glabripennis ). Dhandapani et al (2020a) exposed A. glabripennis larvae to dietary dsRNA ( in vitro synthesized) in sucrose solution for 3 days, and mortality was monitored up to 10 days. The authors observed (depending on target gene) 17–25%, 50–67%, and 75–90% larval mortality associated with force-fed doses of 2, 5, and 10 μg dsRNA/day, respectively.…”

Section: Observed Rnai Sensitivities In Coleopteramentioning

confidence: 99%

Highly Variable Dietary RNAi Sensitivity Among Coleoptera

Willow

Veromann

2021

Front. Plant Sci.

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Many herbivorous beetles (Order Coleoptera) contribute to serious losses in crop yields and forest trees, and plant biotechnology solutions are being developed with the hope of limiting these losses. Due to the unprecedented target-specificity of double-stranded RNA (dsRNA), and its utility in inducing RNA interference (RNAi) when consumed by target pest species, dsRNA-based plant biotechnology approaches represent the cutting edge of current pesticide research and development. We review dietary RNAi studies in coleopterans and discuss prospects and future directions regarding RNAi-based management of coleopteran plant pests. Herein, we also provide a balanced overview of existing studies in order to provide an accurate re-assessment of dietary RNAi sensitivity in coleopterans, despite the limitations to the existing body of scientific literature. We further discuss impediments to our understanding of RNAi sensitivity in this important insect order and identify critical future directions for research in this area, with an emphasis on using plant biotechnology approaches.

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Section: Observed Rnai Sensitivities In Coleopteramentioning

confidence: 99%

Highly Variable Dietary RNAi Sensitivity Among Coleoptera

Willow

Veromann

2021

Front. Plant Sci.

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“…Due to the high specificity and efficiency of RNAi, more and more research groups tried to develop new pest control methods based on this technology and the latest research results showed that inhibiting the expression of important genes in the growth and development process of insects could cause growth and development disorders or death of insects. Many relevant target genes were screened, such as the inhibitor of apoptosis (IAP), vacuolar sorting protein SNF7 (SNF7), and snakeskin (SSK) of Anoplophora glabripennis (Dhandapani et al, 2020); the chitinase (Sf-CHI), chitin synthase B (Sf-CHSB), sugar transporter SWEET1 (Sf-ST), and hemolin (Sf-HEM) of Spodoptera frugiperda (Wan et al, 2021); the leucine-rich repeat-containing (LGR2) of Hyphantria cunea (Sun et al, 2021) and so on. All of above is the strong evidence that RNAi can be used as a new method for pest control.…”

Section: Introductionmentioning

confidence: 99%

Five Visual and Olfactory Target Genes for RNAi in Agrilus Planipennis

Fan

Zhang

et al. 2022

Front. Genet.

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RNA interference (RNAi) is a widely used technique for gene function researches and recently pest controls. It had been applied in emerald ash borer (EAB Agrilus planipennis) larvae and adults, and achieved significant interference effects, whether by ingesting or microinjecting. Feeding in the phloem and cambial regions, the larvae of A. planipennis are difficult to be controlled by conventional insecticides, so adult stage is the critical stage for EAB control. However, the target genes of adult stage of A. planipennis need to be further screened. Here, we preliminarily screened five potential target genes of vision and olfaction for RNAi in A. planipennis. Three odorant binding proteins (OBPs) and three opsins, which expressed significantly different between newly emerged and sexually mature EABs (OBP5, OBP7, OBP10, LW opsin 1 and UV opsin 2) or highly in sexually mature male EAB (UV opsin 3), were selected as targets to design primers for gene silencing. After dsRNA injection, the gene expression levels were determined by real-time quantitative PCR. We found that the expression levels of five genes were significantly down-regulated, during the 4 days after dsRNA injection. Among these genes, the expression of LW opsin 1 was down-regulated the most, causing a reduction of 99.1% compared with the control treated with EGFP dsRNA, followed by UV opsin 3 (97.4%), UV opsin 2 (97.0%), OBP7 (96.2%), and OBP10 (88.7%). This study provides a basis for further RNAi-based new controlling method development of A. planipennis at adult stage.

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“…Anoplophora glabripennis (Coleoptera: Cerambycidae) (Asian long-horned beetle, ALB) is an important wood-boring pest of poplar, willow, acer, elm, buckeye and birch trees (Faccoli & Favaro, 2016;Gao & Li, 2001;Gao et al, 2000Gao et al, , 2009Haack et al, 2010;Tang et al, 1996). The larvae tunnel into the host wood and infested trees is highly susceptible to wind damage, reducing their economic and ecological value (Dhandapani et al, 2020;Gao & Li, 2001). During the mid-to late twentieth-century ALB caused serious economic and ecological damage to poplar shelterbelts in northwestern China (Luo et al, 2002;Lv et al, 2004), and even today, it remains a major problem on Salix, Ulmus and Populus species in Inner Mongolia Autonomous Region, Gansu Province and Xinjiang Uygur Autonomous Region (Golec et al, 2018;Wang et al, 2016;Yue et al, 2011).…”

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confidence: 99%

“…Since it was found in North America in the late 1990s, ALB has been listed as an important quarantine forest pest globally (Haack et al, 2010). Because it is very difficult to control, it continues to pose a significant risk to deciduous hardwood tree species around the world (Dhandapani et al, 2020;Faccoli & Favaro, 2016;Pedlar et al, 2020).…”

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confidence: 99%

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Anoplophora glabripennis: Host choice, oviposition and performance of new hatched larvae on ‘resistant’ poplar species

Yan

Zhang

Liu

et al. 2021

J Applied Entomology

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Most western regions of China are arid or semi-arid. To reduce encroachment by sandstorms in these areas, tens of thousands of forest belts have been established since the 1970s. The majority of these forests are comprised of different varieties or clones of poplar which are tall, fast-growing and resistant to environmental stress (Lv et al., 2000). To date, poplar is still the first choice when planting

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Orally delivered dsRNA induces knockdown of target genes and mortality in the Asian long‐horned beetle, Anoplophora glabripennis

Abstract: The Asian long-horned beetle (ALB) Anoplophora glabripennis is a serious invasive forest pest in several countries, including the United States. Methods available to manage or eradicate this pest are extremely limited, but

Cited by 18 publications

References 22 publications

Highly Variable Dietary RNAi Sensitivity Among Coleoptera

Highly Variable Dietary RNAi Sensitivity Among Coleoptera

Five Visual and Olfactory Target Genes for RNAi in Agrilus Planipennis

Anoplophora glabripennis: Host choice, oviposition and performance of new hatched larvae on ‘resistant’ poplar species

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