Ordered coagulation and gel formation

Thiele, Heinrich

doi:10.1039/df9541800294

Cited by 26 publications

(18 citation statements)

References 4 publications

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“…Tube-like pores with controlled orientation inside the scaffold may support homogeneous cell seeding as well as a sufficient nutrient supply and may facilitate blood vessel ingrowth. The generation of orientated pores within alginate hydrogels can be achieved by taking advantage of the principle of diffusion-controlled ionotropic gelation (Thiele, 1954). This phenomenon comprises the controlled diffusion of di-or trivalent metal cations into an alginate sol, leading to parallel pores up to several centimeters in length, which are orientated in the direction of diffusion.…”

Section: Introductionmentioning

confidence: 99%

Proliferation and osteogenic differentiation of human bone marrow stromal cells on alginate-gelatine-hydroxyapatite scaffolds with anisotropic pore structure

Bernhardt

Despang

Lode

et al. 2009

J Tissue Eng Regen Med

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Porous mineralized scaffolds are required for various applications in bone engineering. In particular, tube-like pores with controlled orientation inside the scaffold may support homogeneous cell seeding as well as sufficient nutrient supply and may facilitate blood vessel ingrowth. Scaffolds with parallely orientated tube-like pores were generated by diffusion-controlled ionotropic gelation of alginate. Incorporation of hydroxyapatite (HA) during the gelation process yielded stable scaffolds with an average pore diameter of approximately 90 microm. To evaluate the potential use of alginate-gelatine-HA scaffolds for bone tissue engineering, in vitro tests with human bone marrow stromal cells (hBMSCs) were carried out. We analysed biocompatibility and cell penetration into the capillary pores by microscopic methods. hBMSCs were also cultivated on alginate-gelatine-HA scaffolds for 3 weeks in the presence and absence of osteogenic supplements. We studied proliferation and osteogenic differentiation in terms of total lactate dehydrogenase (LDH) activity, DNA content and alkaline phosphatase (ALP) activity and found a 10-14-fold increase of cell number after 2 weeks of cultivation, as well as an increase of specific ALP activity for osteogenic-induced hBMSCs. Furthermore, the expression of bone-related genes [ALP, bone sialoprotein II (BSPII)] was analysed. We found an increase of ALP as well as BSPII expression for osteogenic-induced hBMSCs on alginate-gelatin-HA scaffolds.

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Section: Introductionmentioning

confidence: 99%

Proliferation and osteogenic differentiation of human bone marrow stromal cells on alginate-gelatine-hydroxyapatite scaffolds with anisotropic pore structure

Bernhardt

Despang

Lode

et al. 2009

J Tissue Eng Regen Med

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show abstract

“…Not only polyanions like alginate or carrageenan, but also polycations can be used for the formation of ionotropic gels (Thiele 1954). By application of this fact, we developed an immobilization process for getting spherical chitosan biocatalysts Klein et al 1981).…”

Section: Chitosan Biocatalystsmentioning

confidence: 99%

Enzyme Technology

1983

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“…The gelling is a result of the formation of an egg-box structure consisting of GG block in parallel being held together by divalent cation source such as calcium * author to whom all correspondence should be addressed. (Thiele, 1954, Grant et al 1973, Smidsr~d et al 1983). …”

Section: Introductionmentioning

confidence: 99%