Orf virus infection in sheep or goats

Spyrou, Vassiliki; Valiakos, George

doi:10.1016/j.vetmic.2015.08.010

Cited by 112 publications

(112 citation statements)

References 62 publications

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“…A detailed report regarding orf in small ruminants is presented in another paper of this special issue (Spyrou and Valiakos, 2015). In general, clinical signs of orf consist mainly of skin lesions localised primarily in the lips and the nostrils of lambs, as well as in the vulva and the teats of adult female animals and the prepuce of adult male animals.…”

Section: Clinical Signs Of Orf In Small Ruminantsmentioning

confidence: 99%

Zoonotic risks from small ruminants

Ganter

2015

Veterinary Microbiology

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Section: Clinical Signs Of Orf In Small Ruminantsmentioning

confidence: 99%

Zoonotic risks from small ruminants

Ganter

2015

Veterinary Microbiology

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“…For this reason, a scoring system has been used in many studies (13,21). In the present study, lesions were scored by visual examination of scab formation on a scale of 1 to 4 based on a study by Musser et al (13): 1 -no scab, 2 -mild scab, 3 -moderate scab, and 4 -severe scab.…”

Section: Resultsmentioning

confidence: 99%

Comparison of effectiveness of parenteral lincomycin/spectinomycin combination and dexpanthenol application in goat kids with contagious ecthyma

Kasap

Temizel

Karakuş

et al. 2018

Medycyna Weterynaryjna

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Contagious ecthyma (CE) is a highly contagious viral skin disease that is typically self-limited. Treatment options include topical antiseptics, such as KMNO4, local antibiotics and systemic antibiotics to prevent secondary skin infections. The aim of this study was to compare the effectiveness of the lincomycin/spectinomycin combination and dexpanthenol (Dxp) in goat kids with CE. The study was conducted at a Saanen dairy goat farm in Bursa, Turkey. The owner of the goat herd inquired at the veterinary hospital about the appearance of granulomatous lesions on the muzzles of goat kids. In this study, 24 goat kids (1-month-olds) were used. All animals were subjected to the same conditions. Blood and papule samples were taken from the animals’ lips, muzzle and buccal for virological analysis. Swab samples were taken from the lesions for culture and antibiogram. The animals were divided into three groups. Lesions were clinically scored at days 1, 7 and 15 according to a modified previously used scoring system. Goat kids were equally grouped on the basis of lesions on the buccal, lips and muzzle. The animals in group A received 15 mg/kg lincomycin/spectinomycin combination (Lypectin®, Vilsan) intramuscularly for 3 consecutive days, group B received 20 mg/kg Dxp (Bepanthen® amp, Bayer, Germany) intramuscularly for 3 consecutive days, and group C received 0.9% NaCl (2 ml), the control treatment. Clinical recoveries were almost equal in all groups, but by day 14, group A showed better recovery than group B and group C. Both study groups also showed better results than the control group for all days. In conclusion, we believe that the lincomycin/spectinomycin combination or dexpanthenol can be useful in the supplementary treatment of CE in goat kids..

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“…These results suggest that the procedure established here can be carried to completion in the field and could be a powerful tool for on-site diagnosis in remote mountainous areas where Japanese serows are living. Because orf virus is maintained mainly in sheep and goats [17, 29], this procedure could also be useful for on-site diagnosis of infection in domestic animals on farms and pastures.…”

Section: Discussionmentioning

confidence: 99%

Establishment of an on-site diagnostic procedure for detection of orf virus from oral lesions of Japanese serows (<i>Capricornis crispus</i>) by loop-mediated isothermal amplification

Inoshima

Takasu

Ishiguro

2016

The Journal of Veterinary Medical Science

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Orf virus infection has been prevalent continuously in the population of wild Japanese serows (Capricornis crispus), goat-like grazing cloven-hoofed mammal species that live mainly in mountainous areas of Japan. Currently, definitive diagnosis of infection requires time-consuming laboratory work. To diagnose rapidly on-site, we developed a field-friendly procedure for the detection of orf virus from oral cavity lesions. DNA was extracted from goat saliva spiked with orf virus as a proxy for Japanese serows by a commercial kit without the use of electricity, and the quality of the extracted DNA was evaluated by conventional polymerase chain reaction (PCR). Extracted DNA was amenable to DNA amplification, the same as when extracted in a laboratory. Next, to find optimal conditions for DNA amplification by loop-mediated isothermal amplification (LAMP), Bst and Csa DNA polymerases and 3 colorimetric indicators for visual diagnosis, hydroxy naphthol blue (HNB), malachite green and D-QUICK, were compared using a portable cordless incubator. The combination of Bst or Csa DNA polymerase with HNB was found to be easiest for visual diagnosis by the naked eye, and viral DNA was successfully amplified from all orf virus strains used. These results suggest that the procedure established here can work completely on-site and can be useful for definitive diagnosis and differentiation of orf virus infection in Japanese serows in remote mountainous areas.

show abstract

Orf virus infection in sheep or goats

Cited by 112 publications

References 62 publications

Zoonotic risks from small ruminants

Zoonotic risks from small ruminants

Comparison of effectiveness of parenteral lincomycin/spectinomycin combination and dexpanthenol application in goat kids with contagious ecthyma

Establishment of an on-site diagnostic procedure for detection of orf virus from oral lesions of Japanese serows (<i>Capricornis crispus</i>) by loop-mediated isothermal amplification

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