Innovative Sealing of the Spleen in Minimally Invasive Pediatric Surgery ‘Surgical inviolability’ characterizes surgery on the spleen – also in the era of minimally invasive surgery (MIS). The organ’s immunological value was recognized gradually and therewith also the importance of conserving the organ. Imaging techniques, which are used for follow-up, effected more conservative, conserving regimens while, in the end, surgical advances in tissue management led to conserving MIS procedures. Tissue sealing is an essential tool for MIS organ conservation and can be carried out in various forms. Experimental evaluation of fleece-bound sealing (ready-to-use vs on-spot) demonstrated a significantly higher adhesive strength for collagen fleeces that are ready-coated with a fibrinogen-based sealant (TachoComb(r)) than carrier systems which are impregnated by hand with liquid fibrin glue (50.2 vs 23.0 hPa, p < 0.0001). Pure liquid sealing exhibits a surprisingly low adhesive strength (4.1 hPa) and should not be employed on stressed surfaces. The development of an applicator for implementing fleece-bound sealing in MIS was necessary in order to carry out large-area hemostasis/sealing of organs with variable topography. The 10/12-mm instrument, AMISA, is adjustable thanks to its Bowden mechanics and the jib which holds the fleece; it can be completely rotated and swivelled, with degrees of freedom of 2 ×130°. We studied a pediatric collective (University of Erlangen–Nürnberg, 1993 to 2000, 192 patients, mean age 12.2 years) with blunt abdominal trauma, 80 of these patients having splenic trauma. In general, we performed surgery on 25 of these children (31,3%) and of this group; 29.6% had isolated splenic trauma requiring surgery (88.2% MIS), and 39.1% had additional multitraumatization (22.2% MIS). MIS management of the spleen was always carried out with the aid of the AMISA and fleece-bound sealing. Splenectomy was not necessary in that collective, and the patients’ postoperative courses were without complications. The mean stay in hospital was 11 days. Innovative fleece-bound tissue sealing has made it possible to distinctly expand the indications for MIS, and in operable situations in the scope of emergency laparoscopy, this type of sealing can be employed successfully for the management of splenic ruptures.