Organic biopolymers of venus clams: Collagen-related matrix in the bivalve shells with crossed-lamellar ultrastructure

Agbaje, Oluwatoosin B. A.; Dominguez, J. Gabriel; Jacob, Dorrit E.

doi:10.1016/j.bbrep.2021.100939

Cited by 7 publications

(7 citation statements)

References 41 publications

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“…Calcofluor White is widely used for the rapid identification of chitin in chitin-containing organisms, i.e. fungi [15,57], chytrid parasites on phytoplanktonic eukaryotes and prokaryotes [58], and in the shells of molluscs [20,22,59]. In this study, chitinase perforated and/or degraded the studied samples, thus confirming the presence of a chitin matrix in the shells of brachiopods.…”

Section: Discussionsupporting

confidence: 73%

“…1) show absorption bands that are typical of chitin (N-acetyl-D-glucosamine). The band assignments are listed in Table 1, where they are compared with the spectra of standard a-chitin and squid pen bchitin, and literature data [1,20,[47][48][49][50][51][52]. The spectra of the organic fibres reveal the presence of C=O (carbonyl) functional groups based on the amide I stretching vibration at * 1654 cm -1 , and correspond primarily to the b-form of chitin.…”

Section: Vibrational Spectroscopy-microraman and Ftir Spectroscopymentioning

confidence: 99%

“…4). Calcofluor White forms hydrogen bonds with b-(1-3)-and b-(1-4)-linked polysaccharides [20], Figure 2 FTIR spectra of chitin fibre from brachiopod shells: Recent Lingula anatina (RL), fresh Lingula anatina (FL), recent Discinisca tenuis (DT), and pure b-chitin (bC) and a-chitin (aC) as a reference. a Shows a larger wavenumber range (4000-600 cm -1 ) than the expanded range (1800-600 cm -1 ) in (b).…”

Section: Biochemical Analyses Of Chitin Matrixmentioning

confidence: 99%

“…In the layered cuticle of arthropod exoskeletons, chitin is cross-linked with substantial amounts of proteins to form variably oriented yet organized fibres [17,18]. In the matrix sheets of calcareous bivalve shells, the core polysaccharide framework is composed of interlamellar structure of b-chitin, and is frequently reinforced and/or composited with extracellular matrix [4,19,20], other biomolecules include glycosaminoglycans [21][22][23], and/or inorganic components [2,4]. These composite materials are high-strength yet lightweight, and provide functional flexibility, support ultrastructures, mechanical stability and compressive strength [2,24,25].…”

Section: Introductionmentioning

confidence: 99%

“…d, C-O-C glycosidic bond 856 C-C s, C-O b, C--C ring d 432 C-C-C ring d*Shoulder, s = stretching, ass = asymmetrical stretching, ss = symmetrical stretching, b = bending, ip = in-plane-bending, ab = antisymmetric bending, w = wagging, sb = symmetric bending, d = deformation, t = twisting, r = rocking, op = out-of-plane bending. Band assignments were carried out using data from the literature [1,20,[47][48][49][50][51][52]. …”

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confidence: 99%

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Biomacromolecules in recent phosphate-shelled brachiopods: identification and characterization of chitin matrix

et al. 2021

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Phosphate-shelled brachiopods differ in filter-feeding lifestyle, with Lingula anatina an active infaunal burrower, and Discinisca tenuis a shallow marine epibenthic animal. The shells of these animals are built of organophosphatic constituents, the organic fibres/sheets reinforced with calcium phosphate to provide a sophisticated ultrastructural robustness. This investigation examined the nature of the organic fibres in order to improve understanding of how living organisms produce hierarchically structured biomaterials. Unlike powdered samples commonly used in previous studies, organic fibres were isolated for the first time and the shell fractions were purified, in order to study the content and nature of the biopolymer fibres. Biochemical methods including Calcofluor staining revealed a chitin matrix. Ultrastructural analysis, thermal gravimetric analysis, and spectroscopic analyses show that the core polysaccharide framework is composed of layers of β-chitin sheets and/or fibrils that are coated with a fibrous organic matrix. There is more chitin matrix in the L. anatina shells (26.6 wt.%) compared to the D. tenuis shells (12.9 wt.%). Taken together, the data show that the chitin matrix contributes to increased skeletal strength, making L. anatina highly adapted for life as an active burrower. In comparison, D. tenuis contains less chitin and lives as attached epibenthos in a shallow marine environment. Graphical abstract First spectroscopic evidence of β-chitin sheets in recent organophosphatic brachiopods

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Section: Discussionsupporting

confidence: 73%

Section: Vibrational Spectroscopy-microraman and Ftir Spectroscopymentioning

confidence: 99%

Section: Biochemical Analyses Of Chitin Matrixmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

mentioning

confidence: 99%

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Biomacromolecules in recent phosphate-shelled brachiopods: identification and characterization of chitin matrix

et al. 2021

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Molecular Features Associated with Resilience to Ocean Acidification in the Northern Quahog, Mercenaria mercenaria

et al. 2022

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Kinetics of Calcite Nucleation onto Sulfated Chitosan Derivatives and Implications for Water–Polysaccharide Interactions during Crystallization of Sparingly Soluble Salts

Knight,

Mondal,

Han

et al. 2024

Crystal Growth & Design

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Anionic macromolecules are found at sites of CaCO 3 biomineralization in diverse organisms, but their roles in crystallization are not well-understood. We prepared a series of sulfated chitosan derivatives with varied positions and degrees of sulfation, DS(SO 3 − ), and measured calcite nucleation rate onto these materials. Fitting the classical nucleation theory model to the kinetic data reveals the interfacial free energy of the calcite−polysaccharide−solution system, γ net , is lowest for nonsulfated controls and increases with DS(SO 3 − ). The kinetic prefactor also increases with DS(SO 3 − ). Simulations of Ca 2+ −H 2 O−chitosan systems show greater water structuring around sulfate groups compared to uncharged substituents, independent of sulfate location. Ca 2+ −SO 3 − interactions are solvent-separated by distances that are inversely correlated with DS(SO 3 − ) of the polysaccharide. The simulations also predict SO 3 − and NH 3 + groups affect the solvation waters and HCO 3 − ions associated with Ca 2+ . Integrating the experimental and computational evidence suggests sulfate groups influence nucleation by increasing the difficulty of displacing near-surface water, thereby increasing γ net . By correlating γ net and net charge per monosaccharide for diverse polysaccharides, we suggest the solvent-separated interactions of functional groups with Ca 2+ influence thermodynamic and kinetic components to crystallization by similar solvent-dominated processes. The findings reiterate the importance of establishing water structure and properties at macromolecule−solution interfaces.

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Organic biopolymers of venus clams: Collagen-related matrix in the bivalve shells with crossed-lamellar ultrastructure

Cited by 7 publications

References 41 publications

Biomacromolecules in recent phosphate-shelled brachiopods: identification and characterization of chitin matrix

Biomacromolecules in recent phosphate-shelled brachiopods: identification and characterization of chitin matrix

Molecular Features Associated with Resilience to Ocean Acidification in the Northern Quahog, Mercenaria mercenaria

Kinetics of Calcite Nucleation onto Sulfated Chitosan Derivatives and Implications for Water–Polysaccharide Interactions during Crystallization of Sparingly Soluble Salts

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