Organic compounds in the extrapalial fluid and haemolymph of Anodonta cygnea (L.) with emphasis on the seasonal biomineralization process

Moura, Gabriela; Vilarinho, Laura; Santos, António Carvalho; Machado, Jorge

doi:10.1016/s0305-0491(99)00192-3

Cited by 46 publications

(29 citation statements)

References 27 publications

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“…Conversely, a gradual increase of cellular volume in the winter and spring seasons associated with higher levels of organic materials such as mucopolysaccharides correlates with the increment of shell calcification in this period. This agrees with the high metabolic activity in A. cygnea based on increasing levels of organic materials, namely glycosaminoglycans (Moura et al 2000b;Lopes-Lima et al 2005), chitin (Moura et al 2000b) and proteins (Moura et al 2000b) during the year. The larger cellular volumes in this period may reflect increasing intracellular calcium ion concentrations and organic secretions inside the cells which probably raise the osmotic pressure and cause water to enter the cells.…”

supporting

confidence: 80%

Correlation between the morpho-cytohistochemistry of the outer mantle epithelium ofAnodonta cygneawith seasonal variations and following pollutant exposure

Lopes‐Lima

Moura

Pratoomchat

et al. 2006

Marine and Freshwater Behaviour and Physiology

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Seasonal and experimental conditions induce morphological and cytochemical variations in the outer mantle epithelium (OME) of the freshwater bivalve Anodonta cygnea, probably influencing the shell calcification mechanism. In this study, OME samples were taken from untreated animals in autumn, winter, spring and summer as well as from animals exposed to divalent metals (cadmium, chromium, lead, copper and zinc) and pesticides (setoxidim and dimethoate) and observed by light microscopy. The present results showed that OME cells have larger cell volumes and increased amounts of secreted macromolecules during spring and summer than in autumn and winter. This correlates with higher shell calcification rates in spring and summer and lower shell calcification rates in autumn and winter. The experiments showed that incubation with pollutants for 8 months dramatically reduced the cellular volume so that the density of cytoplasmic material appeared higher that in the control samples. The pronounced changes in OME cells suggest a significant decrease in secretory activity following exposure to toxic agents and this has implications for the shell calcification process.

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supporting

confidence: 80%

Correlation between the morpho-cytohistochemistry of the outer mantle epithelium ofAnodonta cygneawith seasonal variations and following pollutant exposure

Lopes‐Lima

Moura

Pratoomchat

et al. 2006

Marine and Freshwater Behaviour and Physiology

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“…Calcium carbonate/phosphate crystals are well regulated, theoretically through an ionotropic or 0376-7388/$ -see front matter © 2010 Elsevier B.V. All rights reserved. doi:10.1016/j.memsci.2010.08.002 stereochemical mechanism [7][8][9][10]. According our studies [9][10][11][12][13][14], the freshwater mussel Anodonta cygnea is a good model to study biomineralization processes due to have large volume biomineralization compartments with easy access to their organic matrixes.…”

Section: Introductionmentioning

confidence: 94%

“…doi:10.1016/j.memsci.2010.08.002 stereochemical mechanism [7][8][9][10]. According our studies [9][10][11][12][13][14], the freshwater mussel Anodonta cygnea is a good model to study biomineralization processes due to have large volume biomineralization compartments with easy access to their organic matrixes. In these animals, biomineralization events occur in two different biomineralization compartments, each with a specific biological fluid, haemolymph and extrapallial fluid, involved in mineral production at the microspherules (mineral concretions) level [9,10] and in the shell calcification respectively.…”

Section: Introductionmentioning

confidence: 94%

“…Although the fluids act at different levels of calcification, their organic and inorganic composition is very similar and balanced either by transport across the mantle barrier or by mantle cellular synthesis. Proteins, glycosaminoglycans (GAGs) and hexosamines (chitin) are the major organic constituents known to be important for biomineralization [9,10].…”

Section: Introductionmentioning

confidence: 99%

“…According our studies [9][10][11][12][13][14], the freshwater mussel Anodonta cygnea is a good model to study biomineralization processes due to have large volume biomineralization compartments with easy access to their organic matrixes. In these animals, biomineralization events occur in two different biomineralization compartments, each with a specific biological fluid, haemolymph and extrapallial fluid, involved in mineral production at the microspherules (mineral concretions) level [9,10] and in the shell calcification respectively. Although the fluids act at different levels of calcification, their organic and inorganic composition is very similar and balanced either by transport across the mantle barrier or by mantle cellular synthesis.…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

The effects of Anodonta cygnea biological fluids on biomineralization of chitosan membranes

Lopes

Lopes‐Lima

Boboş

et al. 2010

Journal of Membrane Science

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a b s t r a c tThe use of chitosan membranes, a low-cost, biocompatible material with promising capabilities in biomedical applications has already been fairly investigated in other fields with good results. Here, the aim was the in vitro mineralization assays accomplished with the chitosan membranes incubated in the control calcium and phosphate solutions on "Ussing" chambers. Biological fluids from Anodonta cygnea were added to the control solutions under different physical-chemical parameters such as pH, synthetic and natural organic compounds. Some organic compounds analyses in the solutions as well as the chitosan membrane SEM-EDS, XRD and FTIR observations were carried out at the end of experimental period. From the experiments it was shown that the chitosan membrane may act as a selective epithelium with preferred ionic movements. Particular mineral deposits, in the chitosan membrane, with hexagonal hydroxiapatite crystals, occurred only at pH 6 with HCL or succinic acid. Biological fluids of A. cygnea induced an increase in number and size of hexagonal crystals forming specific rosaceous structures, especially at pH 6 with succinic acid. It seems that proteins, GAGs and lipids can be involved on this process, since there is a significant reduction on its contents after experiments. So, further applied research with these materials can be a promising subject.

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MSP22.8 is a protease inhibitor‐like protein involved in shell mineralization in the edible mussel Mytilus galloprovincialis

Calvo-Iglesias

Pérez-Estévez²,

González-Fernández

2017

FEBS Open Bio

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The mussel shell protein 22.8 (MSP22.8) is recognized by a monoclonal antibody (M22.8) directed against larvae of the mussel Mytilus galloprovincialis. After being secreted by cells of the mantle‐edge epithelium into the extrapallial (EP) space (the gap between the mantle and the shell), the protein is detected in the extrapallial fluid (EPF) and EP hemocytes and finally becomes part of the shell matrix framework in adult specimens of M. galloprovincialis. In the work described here, we show how MSP22.8 is detected in EPF samples from different species of mussels (M. galloprovincialis, Mytilus edulis, and Xenostrobus securis), and also as a shell matrix protein in M. galloprovincialis, Mytilus chilensis, and Perna canaliculus. A multistep purification strategy was employed to isolate the protein from the EPF, which was then analyzed by mass spectrometry in order to identify it. The results indicate that MSP22.8 is a serpin‐like protein that has great similarity with the protease inhibitor‐like protein‐B1, reported previously for Mytilus coruscus. We suggest that MSP22.8 is part of a system offering protection from proteolysis during biomineralization and is also part of the innate immune system in mussels.

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Organic compounds in the extrapalial fluid and haemolymph of Anodonta cygnea (L.) with emphasis on the seasonal biomineralization process

Cited by 46 publications

References 27 publications

Correlation between the morpho-cytohistochemistry of the outer mantle epithelium ofAnodonta cygneawith seasonal variations and following pollutant exposure

Correlation between the morpho-cytohistochemistry of the outer mantle epithelium ofAnodonta cygneawith seasonal variations and following pollutant exposure

The effects of Anodonta cygnea biological fluids on biomineralization of chitosan membranes

MSP22.8 is a protease inhibitor‐like protein involved in shell mineralization in the edible mussel Mytilus galloprovincialis

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