Nonylphenols (NPNs) are persistent endocrine disruptors and their release into the environment is causing increasing concern about their impact on human health. Herein, an ultrasensitive electrochemical immunosensor was developed for the detection of NPNs in the leachates from 61 instant noodle containers (INCs) from 8 countries across Southeast Asia. Gold nanoclusters (AuNCs) were self‐assembled with reduced graphene oxide (rGO; polyethylenimine–rGO) and used to modify a glassy carbon electrode (GCE), which showed excellent electrical conductivity. An anti‐NPN antibody was then immobilized on the AuNCs and, if it specifically bound NPN, the reduction in conductivity of the GCE was remarkable. The designed immunosensor has a low detection limit (5.25 ng L−1) and high sensitivity for NPNs in the leachates of INCs. Remarkably, the leaching of estrogen‐like compounds from different plastics of INCs and the correlation between NPN content and total estrogenic activity were thoroughly investigated. High temperatures caused polyethylene and polystyrene INCs to release more estrogen‐like compounds than that of polypropylene INCs; this increased release of NPNs was associated with higher estrogen activity in living cells. These data fill the gap in human and environmental exposure to estrogen‐like compounds through INCs.