Organic Extracts of Pelargonium graveolens: Phenol Content, Anti-oxidant and Anti-bacterial Activities

Sompaga, Saraswathi; Jyothi, B.; Chekuri, Sudhakar; Baburao, Nirmala; Anupalli, Roja Rani

doi:10.9734/ejmp/2016/29040

Cited by 1 publication

(3 citation statements)

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“…The fresh leaf of the plant were washed with distilled water, dried at room temperature and ground into fine powder with S4 Drosky grinder and finally stored in sealed bottles at room temperature. Plant material (leaf) was extracted following the method of Sompaga et al 13 with slight modifications. The pulverized plant material (P. graveolens) of about 0.5-3 g was immersed in sufficient volumes each of 50-100 mL methanol, in the airtight bottomed vessel for 12-24 h on the shaker at 200 rpm and was optimized with RSM.…”

Section: Plant Materials Selection and Treatmentmentioning

confidence: 99%

“…These species usually grow within short grasslands and sometimes with shrubs and trees on stony soil varying from sand to clay-loam, shale or basalt. The plants are evergreen when cultivated but die back in natural droughts and winter 13 .…”

Section: Introductionmentioning

confidence: 99%

“…Traditionally, Pelargonium species have been used to treat wounds, eczema, ulcers, acne, bruises and broken capillaries, liver, kidney functions, blood regulation and depression 12,13 . Other properties include analgesic, anticancer, antifungal and treating some hormonal problems 13 .…”

Section: Introductionmentioning

confidence: 99%

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Gc-MS Analysis and Extraction Optimization of Bioactive Compounds From Pelargonium Graveolens Lher Methanolic Extract and Their Activities as Pharmacological Agents

Makanyane¹,

Ejidike²,

Ssemakalu³

et al. 2019

Int. Res. J. Pharm.

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Poly phenols are multifunctional secondary metabolites in plants. Less information is available concerning the statistical yield, optimal extraction method and pharmacological activity with regards to active compounds from Pelargonium graveolens LʼHér leaf. Therefore, Box-Behnken design was used to optimize extraction parameters such as the speed of agitation (rpm), the volume of methanol solvent (mL), time of extraction (h) and mass of plant material (g). A polynomial quadratic model produced satisfactory fitting of experimental data: % yield (Y): R 2 = 0.9943, total phenols (TPC): R 2 = 0.9621 and total flavonoids (TFC): R 2 = 0.9873 at p < 0.05. The antioxidant activities of optimized methanol extract and controls were evaluated by DPPH, ABTS scavenging, and ferric reducing power in vitro assays. The kinetics of the lipid protective activity was studied and fitted into a pseudosecond-order kinetic model. Total antioxidant capacity (TAC) of the extract and gallic acid showed donating potential of 0.35 ± 0.10 mg QE/g and 0.35 ± 0.10 mg QE/g, respectively. The best MIC value of 1.56 mg/mL was against E. coli. The cell proliferation and cytotoxic activities of the extracts were evaluated using the Cell Titer® blue cell viability and lactate dehydrogenase assay on HaCaT cells. Bioactive compounds present in the leaf of P. graveolens were identified by GC-MS analysis by methanol extract.

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Section: Plant Materials Selection and Treatmentmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%