Organic honey supplementation reverses pesticide‐induced genotoxicity by modulating DNA damage response

Alleva, Renata; Manzella, Nicola; Gaetani, Simona; Ciarapica, Veronica; Bracci, Massimo; Caboni, Maria; Pasini, Federica; Monaco, Francesca Roversi; Amati, Monica; Borghi, Battista; Tomasetti, Marco

doi:10.1002/mnfr.201600005

Cited by 21 publications

(12 citation statements)

References 51 publications

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“…Malhomme de la Roche et al [15] found that ingestion of green tea protected PBMN cells challenged ex vivo with UV(A)/VIS (ultraviolet(A)/visible) radiation, but only in some subjects, described as responders. Alleva et al [16] gave a honey supplement to humans exposed to pesticides, and found, after two weeks’ supplementation, lower levels of EndoIII- and Fpg-sensitive sites in lymphocytes as well as an enhanced capacity for DNA repair. Dark chocolate induced a transient protection against H 2 O 2 -induced DNA damage in PBMN cells ex vivo [12].…”

Section: Resultsmentioning

confidence: 99%

“…In parallel experiments to their human honey trial, Alleva et al [16] showed that pre-treatment of cells with honey extract protected against pesticide-induced DNA damage and inhibition of DNA repair. Propolis extract (at high concentration) decreased the frequency of γ-ray-induced SBs in fibroblasts [50], and yet—at a much lower concentration—it caused oxidative damage (SBs measured with Fpg and EndoIII together in the comet assay) in a human cancer cell line, which was suppressed by antioxidants or catalase and so was imputed to the production of H 2 O 2 [51].…”

Section: Resultsmentioning

confidence: 99%

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Polyphenols and DNA Damage: A Mixed Blessing

2016

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Polyphenols are a very broad group of chemicals, widely distributed in plant foods, and endowed with antioxidant activity by virtue of their numerous phenol groups. They are widely studied as putative cancer-protective agents, potentially contributing to the cancer preventive properties of fruits and vegetables. We review recent publications relating to human trials, animal experiments and cell culture, grouping them according to whether polyphenols are investigated in whole foods and drinks, in plant extracts, or as individual compounds. A variety of assays are in use to study genetic damage endpoints. Human trials, of which there are rather few, tend to show decreases in endogenous DNA damage and protection against DNA damage induced ex vivo in blood cells. Most animal experiments have investigated the effects of polyphenols (often at high doses) in combination with known DNA-damaging agents, and generally they show protection. High concentrations can themselves induce DNA damage, as demonstrated in numerous cell culture experiments; low concentrations, on the other hand, tend to decrease DNA damage.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Polyphenols and DNA Damage: A Mixed Blessing

2016

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“…13; 2017 Table 1 summarizes the effect of honey and its derivatives on genome health in in vitro studies. Published articles claimed that honey, propolis and bee pollen can reduce the DNA damage induced by genotoxic agent such as benzo (α) pyrene (Russo et al, 2006), peroxynitrate (Luo et al, 2007), hydrogen peroxide (Afroz et al, 2016;Cheng et al, 2013;Habib et al, 2014;Sherin et al, 2015;Zhou et al, 2012), chlorpyrifos (pesticide) (Alleva et al, 2016) and tertiary-butyl-hydroperoxide (Turan et al, 2015). Generally, antioxidant can scavenge free radicals and reduce the potential of oxidative damage in the cell.…”

Section: Alves De Lima Et Al (2005)mentioning

confidence: 99%

“…Propolis also has a potential to increase the expression of DNA repair enzyme such as hOGG-1 and NEIL-1 (Turan et al, 2015). However, the protective effect by honey toward oxidative damage are mostly from flavonoid content in honey and its derivates (Alleva et al, 2016;Cheng et al, 2013Cheng et al, , 2017Russo et al, 2006;Sherin et al, 2015;Tsai et al, 2012). Propolis that contain flavonoid content such as galangin, pinochembrin, and chrysin have capability to reduce oxidative DNA damage thus become the one of beneficial factor of the propolis (Tsai et al, 2012).…”

Section: In Vitro Studiesmentioning

confidence: 99%

“…There are several agent that can cause DNA damage such as mitomycin-C (Kumari et al, 2017), cyclophosphamide (Fahmy et al, 2015), carbon tetrachloride (CCl 4 ) (Cheng et al, 2015), malathion (Kandiel et al, 2014), alloxan (2,4,5,6-tetraoxypyrimidine; 5,6-dioxyuracil) (Oršolić et al, 2013), 2,3,7,8-tetrachlorodibenzo-p-dioxin (Türkez et al, 2013, cadmium (Cavuşoğlu et al, 2009), pesticides (Alleva et al, 2016), peroxynitrate (Luo et al, 2007), hydrogen peroxide (Russo et al, 2006) and dimethylhydrazine (Alves De Lima et al, 2005).…”

Section: In Vivo Studiesmentioning

confidence: 99%

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Current Knowledge on Honey and Its Derivatives with Genomic Stability: A Mini Review

Yaacob¹,

Stanis²,

Rajab³

et al. 2017

JAS

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Genome health is an important factor that plays a role in various degenerative diseases. Instability of genome is the prevalence of mutation within the genome such as changed in nucleic acid and chromosomal arrangement and also the presence of abnormal number of chromosome in cell. Therefore, several method were used to overcome this problem and one of them is by using natural product such as honey, propolis, bee pollen and royal jelly that is high in antioxidant. Those are products that derive from honey bee and had been used as food supplement to increase the quality of life. Therefore, this systematic review provides the updates on the potential of honey bee products to decrease DNA damage both in in vivo and in vitro approaches. Search term of "honey", "propolis", "bee pollen", royal jelly", "DNA damage", "genome integrity", and "telomere" were used for searching purpose in three databases (Scopus, Pubmed and Medline) and also Google Scholar. All the published articles were assessed using PRISMA guidelines and finally after the eligibility process, only 34 published articles were selected for this review. Based on the reports, the product from the honey bee decrease the genome related diseases by reducing the accumulation of free radical, increase the DNA repair protein expression and decrease the telomerase activity in the cell. This provides a large gap in the research field focusing on the effect of those derivatives from bees on genomic stability.

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Mechanism underlying the effect of long‐term exposure to low dose of pesticides on DNA integrity

Alleva

Manzella

Gaetani

et al. 2018

Environmental Toxicology

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Pesticides, including herbicides, insecticides and fungicides, are widely used in intensive agriculture. Recently, the long-term effects of pesticide exposure were found to be associated with many diseases. In this study, we evaluated the long-term effect of low-level exposure to a mixture of pesticides on DNA damage response (DDR) in relation to individual detoxifying variability. A residential population chronically exposed to pesticides was enrolled, biological/environmental pesticide levels; paroxonase 1 (PON-1) activity and 192 Q/R polymorphism and DDR were evaluated at three different periods of pesticide exposure. OGG1-dependent DNA repair activity was decreased in relation to pesticide exposure. The increase of DNA lesions and pesticide levels in the intensive pesticide-spraying period was independent on PON-1 activity. Next, human bronchial epithelial and neuronal cells were used as a model for in vitro evaluation of the mechanistic effect of pesticides. Pesticides induced mitochondrial dysfunction leading to ROS formation. ROS from mitochondria induced DNA damage, which in turn induced OGG1-dependent DNA repair activity through 8-oxoguanine DNA glycosylase 1 (OGG1) expression and activation. Even though OGG1 was overexpressed, an inhibition of its activity, associated with DNA lesion accumulation, was found at prolonged pesticide-exposure. A post-translational regulation of OGG1 by pesticide may be postulated. Taken together, long-term exposure to low-levels of pesticides affects DDR resulting in accumulation of DNA lesions that eventually may lead to cancer or neurological disorders.

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Organic honey supplementation reverses pesticide‐induced genotoxicity by modulating DNA damage response

Abstract: These results provide new insight regarding the effect of honey containing polyphenols on pesticide-induced DNA damage response.

Cited by 21 publications

References 51 publications

Polyphenols and DNA Damage: A Mixed Blessing

Polyphenols and DNA Damage: A Mixed Blessing

Current Knowledge on Honey and Its Derivatives with Genomic Stability: A Mini Review

Mechanism underlying the effect of long‐term exposure to low dose of pesticides on DNA integrity

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