Organic/inorganic double-layered shells for multiple cytoprotection of individual living cells

Hong, Daewha; Lee, Hojae; Ko, Eun Hyea; Lee, Juno; Cho, Hyeoncheol; Park, Matthew; Yang, Sung Ho; Choi, Insung S.

doi:10.1039/c4sc02789b

Cited by 67 publications

(56 citation statements)

References 34 publications

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“…, HeLa cells), in our previous studies1839. After cell encapsulation with the MSi shells, the cell viability was maintained without noticeable decrease, and yeast@MSi retained their basic metabolism and functions, such as cell division, because small molecules, such as O 2 and nutrients, could pass through the MSi shells35. The UV-Visible and SQUID analyses confirmed that the multinary magnetization of yeast cells was achieved by the reactive LbL method that controlled the number of MNPs deposited onto the cell wall.…”

Section: Discussionmentioning

confidence: 87%

“…, formation of “cell-in-shell” structures) is to temporarily protect the cells against external stress during manipulation and storage, and to endow the cells with exogenous properties, magnetism in this work31323334. Living cells are generally vulnerable to chemical materials and treatments, and, therefore coating materials and procedures should be cytocompatible35363738394041. In this work, we chose polyamine-stabilized MNPs and silica as coating materials, because these materials proved highly cytocompatible, even in the case of mammalian cells ( e.g.…”

Section: Discussionmentioning

confidence: 99%

“…Iron oxide MNPs were prepared by the co-precipitation method35. Briefly, 1.73 g of FeCl 3 ∙6H 2 O and 0.635 g of FeCl 2 ∙4H 2 O (molar ratio, Fe(III):Fe(II) = 2:1) were completely dissolved in 5 mL of distilled water, followed by adding 0.17 mL of 12-M HCl solution with stirring.…”

Section: Methodsmentioning

confidence: 99%

“…In other words, the cell magnetization has so far been limited to binary systems (yes and no from magnetized and non-magnetized cells), but “multinary” magnetization of living cells would allow for multi-level control of magnetized cells. We also envisioned that the multinary incorporation of magnetic nanomaterials into artificial spores (cell-in-shell structures)3132333435 would provide an advanced tool for manipulating the cellular hybrid structures at the single-cell level. In this work, we developed a simple but versatile method for varying the magnetization degrees of individual living cells, as micro-magnets, while maintaining the cell viability.…”

mentioning

confidence: 99%

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Turning Diamagnetic Microbes into Multinary Micro-Magnets: Magnetophoresis and Spatio-Temporal Manipulation of Individual Living Cells

Lee

Hong

Cho

et al. 2016

Sci Rep

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Inspired by the biogenic magnetism found in certain organisms, such as magnetotactic bacteria, magnetic nanomaterials have been integrated into living cells for bioorthogonal, magnetic manipulation of the cells. However, magnetized cells have so far been reported to be only binary system (on/off) without any control of magnetization degree, limiting their applications typically to the simple accumulation or separation of cells as a whole. In this work, the magnetization degree is tightly controlled, leading to the generation of multiple subgroups of the magnetized cells, and each subgroup is manipulated independently from the other subgroups in the pool of heterogeneous cell-mixtures. This work will provide a strategic approach to tailor-made fabrication of magnetically functionalized living cells as micro-magnets, and open new vistas in biotechnological and biomedical applications, which highly demand the spatio-temporal manipulation of living cells.

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Section: Discussionmentioning

confidence: 87%

Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

mentioning

confidence: 99%

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Turning Diamagnetic Microbes into Multinary Micro-Magnets: Magnetophoresis and Spatio-Temporal Manipulation of Individual Living Cells

Lee

Hong

Cho

et al. 2016

Sci Rep

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“…The ESMH was obtained by following our previous report. [33] Yeast@CM was dispersed in an ESMH solution (0.25 mg mL À 1 in the 0.15-M NaCl solution) and gently stirred for 15 min. The ESMH-primed yeast@CM was washed three times with the NaCl solution.…”

Section: Extraction and Characterizations Of High Molecular-weightmentioning

confidence: 99%

Coffee Melanoidin‐Based Multipurpose Film Formation: Application to Single‐Cell Nanoencapsulation

Kim

Han

et al. 2020

ChemNanoMat

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The melanoidin from soluble coffee is utilized as a material‐independent, multipurpose coating material. Instantaneous complexation of the coffee melanoidin (CM) with ferric ion (Fe3+) leads to surface‐adhesive aggregates, inducing sequential film deposition. Various chemical groups of the CM also allow for post‐functionalizations of the CM film, including surface‐initiated, ring‐opening polymerization and bioinspired silicification. In addition, the CM‐based coating is applied to single‐cell nanoencapsulation with a strategy of biphasic interfacial reactions. The method is highly cytocompatible (viability >98%), and the CM shell is cytoprotective against lytic enzymes. The coated cells inherit the characterictics of the CM, such as post‐functionalizability and antioxidant property. Considering that surface‐coating technologies with cytocompatible natural polymers have widely been used for engineering bioentities, the CM‐based coating strategy would provide an advanced option for biomedical applications.

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Bio‐Inspired Synthesis and Application of Functional Inorganic Materials by Polymer‐Controlled Crystallization

Liu

2017

Handbook of Solid State Chemistry

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Biominerals present in organisms are typically inorganic–organic hybrid materials with unique complex form, hierarchically ordered superstructures, and superior materials properties. Bio‐inspired polymer‐controlled crystallization approaches, aiming to transform the optimized designs and formation mechanisms lurking in biominerals to artificial systems, have been successfully applied to the synthesis of a wide range of inorganic materials. In order to avoid duplication with previous reviews, in this chapter we only focus on the new branches of polymer‐controlled crystallization for the design and preparation of functional inorganic materials with novel structures and improved resulting properties. For clear understanding of the interactions between added polymers and growing crystals and their mediation over the final crystal structures, some basic principles of crystallization involving classical and nonclassical pathways are first introduced. Emphasis will be laid on latest developments and important advances of bio‐inspired synthesis of various kinds of functional inorganic materials via polymer‐controlled crystallization. Their resulting improvements in different properties and potential applications in diverse fields of energy transformation and storage, catalysis, sensing, environmental management, (bio)medical science, and so on are subsequently discussed and highlighted. The challenges of current studies and prospects for future research about this new branch topic are finally suggested.

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Organic/inorganic double-layered shells for multiple cytoprotection of individual living cells

Abstract: S. cerevisiae encapsulated with a poly(norepinephrine)/silica double-layered shell showed multiple resistance to enzymatic attack, desiccation, and UV-C irradiation. The biochemical response of the encapsulated yeast may also contribute to the UV-C resistance.

Cited by 67 publications

References 34 publications

Turning Diamagnetic Microbes into Multinary Micro-Magnets: Magnetophoresis and Spatio-Temporal Manipulation of Individual Living Cells

Turning Diamagnetic Microbes into Multinary Micro-Magnets: Magnetophoresis and Spatio-Temporal Manipulation of Individual Living Cells

Coffee Melanoidin‐Based Multipurpose Film Formation: Application to Single‐Cell Nanoencapsulation

Bio‐Inspired Synthesis and Application of Functional Inorganic Materials by Polymer‐Controlled Crystallization

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