Organic nitrogen addition causes decoupling of microbial nitrogen cycles by stimulating gross nitrogen transformation in a temperate forest soil

Lu, Mingzhu; Cheng, Shulan; Fang, Huajun; Meng, Xu; Yang, Yan; Li, Yuna; Zhang, Jinbo; Müller, Christoph

doi:10.1016/j.geoderma.2020.114886

Cited by 22 publications

(10 citation statements)

References 52 publications

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“…It has distinct impacts on mineralization and microbial N immobilization due to soil chemical properties, for example, soil C content, soil N contents, soil C/N ratio, soil organic matter and soil pH [47,65,66]. In this study, we found that the soil C/N ratio (not greater than 25) is a critical factor for extraneous 13 C, 15 N-glycine mineralization (Figure 4).…”

Section: Glycine-addition Uptake By Soil Microorganisms and Controlli...mentioning

confidence: 55%

“…It should be noted that variation in altitudes has been shown to lead to changes not only in temperature but also in factors such as soil properties [46]. It has been found that exogenous organic N generally promoted gross N mineralization in the main temperate forest of Changbai Mountain [47]. However, we lack a clear understanding of how amino acid addition affects gross N transformation under different soil properties.…”

Section: Introductionmentioning

confidence: 95%

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Utilization of Glycine by Microorganisms along the Altitude Changbai Mountain, China: An Uptake Test Using 13C,15N Labeling and 13C-PLFA Analysis

Xue

Zhang

et al. 2022

Forests

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External organic nitrogen (N) inputs can contrastingly affect the transformation and availability of N in forest soils, which is an important potential N resource and is possibly vulnerable to soil properties. Little is known about the transformation and availability of external small molecule organic N in forest soils and the underlying microbial mechanisms. Soil samples from Changbai Mountain at different altitudes (from 750 m to 2200 m) that ranged widely in soil properties were incubated with 13C, 15N-labeled glycine. The fate of 15N-glycine and the incorporation of 13C into different phospholipid fatty acids (PLFAs) were measured at the same time. The addition of glycine promoted gross N mineralization and microbial N immobilization significantly. Mineralization of glycine N accounted for 6.2–22.5% of the added glycine and can be explicable in the light of a readily mineralizable substrate by soil microorganisms. Assimilation of glycine N into microbial biomass by the mineralization-immobilization-turnover (MIT) route accounted for 24.7–52.1% of the added label and was most mightily affected by the soil C/N ratio. We also found that the direct utilization of glycine is important to fulfill microorganism growth under the lack of available carbon (C) at upper elevations. The labeled glycine was rapidly incorporated into the PLFAs and was primarily assimilated by bacteria, indicating that different groups of the microbial community were answerable to external organic N. G+ bacteria were the main competitors for the exogenous glycine. Increased intact incorporation of glycine into microbial biomass and the concentration of PLFAs in general, particularly in G+ bacteria, suggest a diversified arrangement to response changes in substrate availability.

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Section: Glycine-addition Uptake By Soil Microorganisms and Controlli...mentioning

confidence: 55%

Section: Introductionmentioning

confidence: 95%

Utilization of Glycine by Microorganisms along the Altitude Changbai Mountain, China: An Uptake Test Using 13C,15N Labeling and 13C-PLFA Analysis

Xue

Zhang

et al. 2022

Forests

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“…15 N tracing methods could be used to determine soil gross N transformations. Besides, it also provides a mechanistic explanation for altered soil inorganic N (Garousi et al, 2021; Lu et al, 2021). This method is pertinent to understanding the process of altered N dynamic, as demonstrated by previous studies in multiple ecosystems.…”

Section: Introductionmentioning

confidence: 99%

Decrease in soil inorganic nitrogen supply capacity under long‐term areca nut plantations in the tropics

Zhu

Liu

et al. 2022

Land Degrad Dev

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Understanding the dynamics and availability of soil nitrogen (N) affected by the conversion of cropping patterns is critical for environmental sustainability, especially in tropical soils with low fertility and high N loss. In this study, the 15 N tracing technology combined with the 15 N tracer model was used to explore the dynamic change of soil N transformation in long-estabished areca nut (Areca catechu) plantations. Areca nut plantations with different ages (2, 5, 10, 14, and 17 years) and paddy fields in the tropical region of China were studied. The results demonstrated that the gross N mineralization rate (M) of areca nut plantation soil was much lower than that of paddy soil. The NH 4 + immobilization (I NH4 ) rate was also significantly reduced in areca nut plantations. Besides, the O NH4 (autotrophic nitrification) in long-term areca nut planted soil decreased significantly with decreasing ammonia-oxidizing archaea (AOA) and ammonia-oxidizing bacteria (AOB) abundance. Inorganic N supply (INS) capacity of areca nut planted soil was much lower than the paddy soil, indicating declined N supply in long-term areca nut plantations soil. The decline in soil gross N transformations rate and INS capacity of areca nut plantations soil was significantly correlated to reduced levels of TN and soil pH. Thus, agricultural practices that increase the soil pH (e.g., biochar or lime application) and the soil organic matter content (e.g., organic fertilizers) could improve soil INS capacity. K E Y W O R D S 15 N tracing study, gross N transformations, inorganic N supply capacity, long-term areca nut plantations, the 15 N tracer model 1 | INTRODUCTION Driven by economic interests, many agronomic crop farmlands have converted to economic crop farmlands (Lu et al., 2010). The planting area of areca nut, an important economic crop, has increased from 0.56 Â 10 6 ha in 2000 to 1.24 Â 10 6 ha in 2019 worldwide (FAO, 2019). Areca nut yields gradually declined in most regions due to intensive cultivation. Areca nut yield had decreased from 3.23 Â 10 4 kgÁha À1 in 2000 to 2.54 Â 10 4 kg ha À1 in 2019 in China (FAO, 2019). To increase profit, excess fertilizer and water were used in areca nuts plantations, even then, areca nut yield decreased over

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“…Stoichiometry of soil carbon (C), nitrogen (N) and phosphorus (P) is important foundation for understanding biogeochemical processes in terrestrial ecosystems. Increased research attention has been paid to spatial patterns of C, N and P distribution in mountainous forests, and immense variability of their altitudinal distribution has been recognized in various regions [1][2]. There are reports that soil C and N concentrations were higher at medium altitudes than that at low or high altitudes, and P concentrations increased significantly with the rising of altitude, suggesting an increase in N limitation to forests at higher elevations [3][4].…”

Section: Introductionmentioning

confidence: 99%

“…However, current knowledge of the role of slope aspects on these changes as well as soil stoichiometry is limited. The objectives of this study were (1) to characterize soil C, N, and P contents and stoichiometry along altitudinal gradient on sunny and shady slopes in Sygera mountains, and (2) to identify the dominant factors regulating spatial difference in soil C, N, P contents and their stoichiometry.…”

Section: Introductionmentioning

confidence: 99%

Effects of slope aspect on altitudinal pattern of soil C:N:P stoichiometry in alpine forest of Tibet

Guo

He-ping

et al. 2021

E3S Web Conf.

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Knowledge of altitudinal patterns in soil C, N and P distribution is important for understanding biogeochemical processes in mountainous forests, yet the influence of slope aspects on soil stoichiometry has been largely neglected in previous studies. In this paper, a total number of 150 topsoil samples at four altitudes (3700, 3900, 4100, 4380 m a.s.l.) on sunny and shady slopes of Sygera mountains in the Southeastern Tibet were collected. Soil C, N and P contents, and pH, were measured. Soil temperature, moisture and richness of plant species were investigated at each sampling site. The results showed that: 1) in sunny slope, soil C, N and P concentrations increased with the increase in altitude, whereas soil C:N, C:P, and N:P decreased along the altitudinal gradient on s. Soil moisture was the main regulator of soil nutrition and stoichiometric ratios. 2) In shady slope, soil C and N contents had no significant difference along the altitudinal gradient except the higher values at low altitude, whereas soil P increased first and then decreased. Soil C:N increased with the increase in altitude, whereas C:P and N:P decreased first and then increased. Soil temperature and species richness were the main factors influencing soil nutrition and stoichiometric ratios. 3) Decoupling of soil C:N:P stoichiometry was observed in shady slope owing to changes in soil pH and temperature. 4) The rich contents of soil C and P were observed at two slopes along the altitudinal gradient, and high capacity of N supply existed at the topsoil in shady slope. These results suggested that slope aspect plays an important role in shaping the altitudinal pattern of soil C:N:P stoichiometry in mountainous forests.

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Organic nitrogen addition causes decoupling of microbial nitrogen cycles by stimulating gross nitrogen transformation in a temperate forest soil

Cited by 22 publications

References 52 publications

Utilization of Glycine by Microorganisms along the Altitude Changbai Mountain, China: An Uptake Test Using 13C,15N Labeling and 13C-PLFA Analysis

Utilization of Glycine by Microorganisms along the Altitude Changbai Mountain, China: An Uptake Test Using 13C,15N Labeling and 13C-PLFA Analysis

Decrease in soil inorganic nitrogen supply capacity under long‐term areca nut plantations in the tropics

Effects of slope aspect on altitudinal pattern of soil C:N:P stoichiometry in alpine forest of Tibet

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