Organisation of Xanthophyll-Lipid Membranes Studied by Means of Specific Pigment Antisera, Spectrophotometry and Monomolecular Layer Technique Lutein versus Zeaxanthin

Gruszecki, Wiesław I.; Sujak, Agnieszka; Strzałka, Kazimierz; Radunz, Alfons; Schmid, Georg

doi:10.1515/znc-1999-7-810

Cited by 35 publications

(19 citation statements)

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“…Such orientation has been proposed for xanthophylls in a conformation cis [39]. Interestingly, lutein characterised by the rotational freedom around the 6′-7′ single bond is believed to adopt 2 orthogonal orientations: one roughly vertical and the other horizontal to the lipid bilayer [40,41].…”

Section: Localisation Within Model Membranesmentioning

confidence: 85%

Characterisation of Carotenoids Involved in the Xanthophyll Cycle

Kuczyńska¹,

Jemioła-Rzemińska²,

KazimierzStrzalka³

2017

Carotenoids

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Carotenoids are known for versatile roles they play in living organisms; however, their most pivotal function is involvement in scavenging reactive oxygen species (ROS) and photoprotection. In plant kingdom, an important photoprotective mechanism, referred to as the xanthophyll cycle, has been developed by photosynthetic organism to avoid excess light that might lead to photoinhibition and inactivation of photosystems and induce the formation of reactive oxygen species (ROS), resulting in photodamage and long-term changes in the cells caused by oxidative stress. Apart from high-light driven enzymatic conversion of violaxanthin (Viola) to zeaxanthin (Zea) that occurs mostly in higher plants, mosses and lichens, other less known types of the xanthophyll cycle have been hitherto described. The work is aimed at summarising the current knowledge on the pigments engaged in the xanthophyll cycles operating in various organisms.

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Section: Localisation Within Model Membranesmentioning

confidence: 85%

Characterisation of Carotenoids Involved in the Xanthophyll Cycle

Kuczyńska¹,

Jemioła-Rzemińska²,

KazimierzStrzalka³

2017

Carotenoids

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“…The high antioxidant activity of astaxanthin is attributed to the presence of a keto- and OH- group in each ionone ring which allows the molecule to be esterified, and makes it more polar than other carotenoids. Xanthophylls are thought to be located in the cell membrane lipid bilayer with their hydrophobic groups in the aqueous phase or anchored in the polar head region of the lipid bilayer [43]; this increases membrane rigidity and allows them to serve as molecular rivets to impair the entry of small polar molecules such as singlet oxygen.…”

Section: Discussionmentioning

confidence: 99%

Astaxanthin uptake in domestic dogs and cats

et al. 2010

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BackgroundResearch on the uptake and transport of astaxanthin is lacking in most species. We studied the uptake of astaxanthin by plasma, lipoproteins and leukocytes in domestic dogs and cats.MethodsMature female Beagle dogs (18 to 19 mo old; 11 to 14 kg BW) were dosed orally with 0, 0.1, 0.5, 2.5, 10 or 40 mg astaxanthin and blood taken at 0, 3, 6, 9, 12, 18 and 24 h post-administration (n = 8/treatment). Similarly, mature domestic short hair cats (12 mo old; 3 to 3.5 kg body weight) were fed a single dose of 0, 0.02, 0.08, 0.4, 2, 5, or 10 mg astaxanthin and blood taken (n = 8/treatment) at the same interval.ResultsBoth dogs and cats showed similar biokinetic profiles. Maximal astaxanthin concentration in plasma was approximately 0.14 μmol/L in both species, and was observed at 6 h post-dosing. The plasma astaxanthin elimination half-life was 9 to 18 h. Astaxanthin was still detectable by 24 h in both species. In a subsequent study, dogs and cats were fed similar doses of astaxanthin daily for 15 to 16 d and astaxanthin uptake by plasma, lipoproteins, and leukocytes studied. In both species, plasma astaxanthin concentrations generally continued to increase through d 15 or 16 of supplementation. The astaxanthin was mainly associated with high density lipoprotein (HDL). In blood leukocytes, approximately half of the total astaxanthin was found in the mitochondria, with significant amounts also associated with the microsomes and nuclei.ConclusionDogs and cats absorb astaxanthin from the diet. In the blood, the astaxanthin is mainly associated with HDL, and is taken up by blood leukocytes, where it is distributed to all subcellular organelles. Certain aspects of the biokinetic uptake of astaxanthin in dogs and cats are similar to that in humans.

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“…The monolayers were deposited by spreading a proper volume of C 80 /DOPC mixture in chloroform. Surface pressure was measured by the Wilhelmy method (Gruszecki et al, 1999a;1999b). Monomolecular layers at the air-water interface were formed in a 104 0 cm Teflon trough.…”

Section: Methodsmentioning

confidence: 99%

“…On the basis of the results presented in Fig. 1, one 664 T. Janas and others 2000 can calculate the specific molecular area, A M , (Costin & Barnes, 1975;Gruszecki et al, 1999a). The values of A M are found by extrapolation of the linear parts of the isotherms to zero surface pressure ( Fig 3A).…”

Section: Monolayer Experimentsmentioning

confidence: 99%

“…The molecule of hexadecaprenol is composed of 16 isoprene units with the structure: wT 2 C 12 aOH, where w is an isoprene residue, T is a trans-isoprene residue, C is a cis-isoprene residue, a is the a-saturated OH terminal isoprene residue and OH is the hydroxyl group. Monolayer techniques have been applied to the study of the interactions between dioleoylphosphatidylcholine and other lipids (Gaines, 1966;Costin & Barnes, 1975;Gruszecki et al, 1999a) and this technique can be used to investigate the origin and magnitude of the molecular interactions in mixed monolayers. The aim of the present work was to study the influence of polyprenol molecules on the organisation and packing of phospholipid molecules in lipid monolayers and bilayers.…”

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The effect of hexadecaprenol on molecular organisation and transport properties of model membranes.

2000

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The Langmuir monolayer technique and voltammetric analysis were used to investigate the properties of model lipid membranes prepared from dioleoylphosphatidylcholine (DOPC), hexadecaprenol (C 80 ), and their mixtures. Surface pressuremolecular area isotherms, current-voltage characteristics, and membrane conductance-temperature were measured. Molecular area isobars, specific molecular areas, excess free energy of mixing, collapse pressure and collapse area were determined for lipid monolayers. Membrane conductance, activation energy of ion migration across the membrane, and membrane permeability coefficient for chloride ions were determined for lipid bilayers. Hexadecaprenol decreases the activation energy and increases membrane conductance and membrane permeability coefficient. The results of monolayer and bilayer investigations show that some electrical, transport and packing properties of lipid membranes change under the influence of hexadecaprenol. The results indicate that hexadecaprenol modulates the molecular organisation of the membrane and that the specific molecular area of polyprenol molecules depends on the relative concentration of polyprenols in membranes. We suggest that hexadecaprenol modifies lipid membranes by the formation of fluid microdomains. The results also indicate that electrical transmembrane potential can accelerate the formation of pores in lipid bilayers modified by long chain polyprenols.

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Organisation of Xanthophyll-Lipid Membranes Studied by Means of Specific Pigment Antisera, Spectrophotometry and Monomolecular Layer Technique Lutein versus Zeaxanthin

Cited by 35 publications

References 0 publications

Characterisation of Carotenoids Involved in the Xanthophyll Cycle

Characterisation of Carotenoids Involved in the Xanthophyll Cycle

Astaxanthin uptake in domestic dogs and cats

The effect of hexadecaprenol on molecular organisation and transport properties of model membranes.

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