Organization of a Tumor Bank: The Experience of the National Cancer Institute of Mexico

Ruíz-Godoy, Luz; Meneses-García, Abelardo; Suarez-Roa, Lourdes; Enriquez,; Lechuga-Rojas, R; Reyes-Lira, E

doi:10.1159/000292648

Cited by 5 publications

(3 citation statements)

References 44 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…These analyses provided several relevant and, in part, surprising conclusions. Human tissue for research, especially fresh frozen tissue, is a sparse and rate-limiting resource [14], and therefore adequate use of tissue should and can be enforced via structured project management [12,15,16]. Consequently, most of the effort in general focuses on the acquisition, handling and storage of frozen tissue.…”

Section: Discussionmentioning

confidence: 99%

Structural requirements of research tissue banks derived from standardized project surveillance

et al. 2012

View full text Add to dashboard Cite

Tissue banks constitute decisive and rate-limiting resource and technology platforms for basic and translational biomedical research, notably in the area of cancer. Thus, it is essential to plan and structure tissue banking and allocate resources according to research needs, but essential requirements are still incompletely defined. The tissue bank of the National Center of Tumor Diseases Heidelberg (NCT) was founded with the intention to provide tissues of optimal quality and to prioritize the realization of research projects. We analysed its structure and prospective project management registration as well as tracking records for all projects of the NCT tissue bank as of its start in 2005 in order to obtain information that may be relevant for tissue bank planning. All project proposals submitted to the NCT tissue bank (n = 681) were included in the study. For a detailed evaluation of provided services, only projects that were completed until July 2011 (n = 605) were analysed. For these 605 projects, NCT tissue bank provided 769 specific services. In all projects/services, we recorded project leader, type and amount of material provided, type of research (basic/translational), work load of project and project completion. Furthermore, all completed projects were tracked after 90 days according to a standard protocol to determine principal investigators' (PI) satisfaction and quality of the provided material. Until July 2011, 605 projects had been successfully completed as documented by material transfer agreement. Of the projects, 72.7 % addressed basic research, 22.3 % were translational research projects and 3 % concerned epidemiological research; 91 % (n = 546) concerned a single PI and the NTC tissue bank. For these projects, 769 specific services were provided. Of these services, 288 concerned providing formalin-fixed and paraffin-embedded (FFPE) tissue (extracts, full size sections), 126 providing fresh frozen materials (including fresh frozen sections), 137 providing tissue micro-array (TMA)-based sections and 199 providing immunohistochemical services. Project tracking demonstrated that all projects had started within 90 days after reception of the material by the PIs, and PI satisfaction with provided material exceeded 97 %. Standardized registration and tracking provides valuable structural information for planning and financing of tissue banks and allocation of resources. The high number of completed projects as well as high user satisfaction demonstrates that structuring of tissue banks should be preferably research-oriented and highly efficient. The comparable number of requests for FFPE and fresh frozen tissue as well as TMA-based services underpins the need for a broad approach in terms of methods and material types in order to fulfil research needs.

show abstract

Section: Discussionmentioning

confidence: 99%

Structural requirements of research tissue banks derived from standardized project surveillance

et al. 2012

View full text Add to dashboard Cite

show abstract

“…Normal tissue was surgically withdrawn from selected areas of normal breast tissue of 31 cancer patients (age from 27 to 89 years, mean 58±17 years) and stored in liquid nitrogen in the INCAN Tumor Bank for 12-24 months (21). Internal control of sample stability revealed that the contents of several proteins (HIF-1α, GLUT, LDH and ATPase) from fresh samples were similar to those stored in the INCAN Tumor Bank (data not shown).…”

Section: Methodsmentioning

confidence: 95%

Identification of a metabolic and canonical biomarker signature in Mexican HR+/HER2−, triple positive and triple-negative breast cancer patients

Pacheco-Velázquez¹,

Gallardo‐Pérez²,

Aguilar-Ponce³

et al. 2014

International Journal of Oncology

View full text Add to dashboard Cite

Infiltrating ductal breast cancer (IDC) is the principal tumor associated-malignancy in Mexican women. In IDC, the development of intermittent hypoxia leads to an adaptive response coordinated by the transcriptional factor HIF-1α. In the present pilot, retrospective/cross-sectional study, the HIF-1α expression was analyzed in 102 tru-cut biopsies from female patients (51 ± 12 years) without previous clinical treatment and compared to 31 normal breast biopsies. The 102 IDC samples corresponded to 56% of HER2-/HR+; 8% of HER2+/HR-; 22% of triple positive (HER2+/HR+); and 14% of triple negative (TN, HER2-/HR-) subtypes. To assess HIF-1α functionality, proteomic and kinetic analysis of glycolytic as well as mitochondrial enzymes, were determined. Validation of HIF-1α as cancer biomarker was assessed by determining the contents of the commonly used biomarkers c-MYC, Ki67, and H- and K-RAS, as well as metastatic and autophagy proteins. Proteomic analysis revealed that HIF-1α, c-MYC, HER2 and COXIV contents were significantly increased in all IDC subtypes vs. normal tissue. The contents and activities of glycolytic proteins were similar between normal and IDC samples, except for HER2-/HR+ where a substantial increase of HKII was observed. Significant increase in 2OGDH and E-cadherin was detected for TN samples vs. other IDC subtypes and for normal samples. These results clearly indicated that HIF-1α + COXIV + c-MYC (+ HER2 for HER2+ subtype) may be useful to depict a breast cancer metabolic marker pattern for diagnosis, whereas the contents of HIF-1α + c-MYC + 2OGDH + E-cadherin may be an alternative useful and reliable signature for TN subtype cancer prognosis.

show abstract

“…Components of such specimens can be used for DNA, ribonucleic acid (RNA) and protein research. 10 , 11 …”

Section: Methodsmentioning

confidence: 99%

Establishment and management of a lung cancer biobank in Eastern China

Zhang

et al. 2015

Thoracic Cancer

View full text Add to dashboard Cite

BackgroundThe prevalence of lung cancer, a highly complex neoplasm, increases annually. Thus, a lung cancer biobank, which stores lung cancer tissue and blood matched according to standard methods, is needed to advance lung cancer research and develop promising therapies.MethodsTo accomplish this aim, we implemented standardized procedures for tissue samples and patient information acquired from consenting donors. The banked tissue includes blood, pleural effusions, and surgical resection samples. An independent information management system was used to match samples and collect data, including clinical cancer manifestation, laboratory tests, and de-identified data about cancer patients.ResultsFrom 2009 to 2013, more than 2000 lung cancer cases were collected. At this time, we have more than 10 000 biological samples stored in our biobank. DNA, ribonucleic acid (RNA), and protein quality were confirmed to be appropriate for clinical and basic research.ConclusionOur standardized, large-scale lung cancer biobank offers high quality cancer research samples for China and the world.

show abstract

Organization of a Tumor Bank: The Experience of the National Cancer Institute of Mexico

Cited by 5 publications

References 44 publications

Structural requirements of research tissue banks derived from standardized project surveillance

Structural requirements of research tissue banks derived from standardized project surveillance

Identification of a metabolic and canonical biomarker signature in Mexican HR+/HER2−, triple positive and triple-negative breast cancer patients

Establishment and management of a lung cancer biobank in Eastern China

Contact Info

Product

Resources

About