Organization of the Bovine α2-Fucosyltransferase Gene Cluster Suggests that the Sec1 Gene Might Have Been Shaped Through a Nonautonomous L1-Retrotransposition Event Within the Same Locus

Saunier, Katiana; Barreaud, Jean-Pierre; Eggen, Andre A.; Oriol, Rafaël; Levéziel, Hubert; Julien, Raymond; Petit, Jean‐Michel

doi:10.1093/oxfordjournals.molbev.a003749

Cited by 9 publications

(8 citation statements)

References 38 publications

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“…The ␣1,2-fucosyltransferases (FUT1, FUT2, and Sec1) (10) and the ␣1,3/4-fucosyltransferases (FUT3-FUT7 and FUT9), resulting mainly from two rounds of whole genome duplication (11), are implicated in terminal fucosylations (12,13), and they are encoded by monoexonic genes (1). The ␣1,6-fucosyltransferase encoded by the FUT8 gene transfers ␣-L-fucose onto the innermost asparagine-linked GlcNAc of the chitobiose disaccharide unit of glycoproteins (14,15).…”

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confidence: 99%

Activity, Splice Variants, Conserved Peptide Motifs, and Phylogeny of Two New α1,3-Fucosyltransferase Families (FUT10 and FUT11)

Mollicone

Moore

Bovin

et al. 2009

Journal of Biological Chemistry

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We report the cloning of three splice variants of the FUT10 gene, encoding for active ␣-L-fucosyltransferase-isoforms of 391, 419, and 479 amino acids, and two splice variants of the FUT11 gene, encoding for two related ␣-L-fucosyltransferases of 476 and 492 amino acids. The FUT10 and FUT11 appeared 830 million years ago, whereas the other ␣1,3-fucosyltransferases emerged 450 million years ago. FUT10-391 and FUT10-419 were expressed in human embryos, whereas FUT10-479 was cloned from adult brain and was not found in embryos. Recombinant FUT10-419 and FUT10-479 have a type II trans-membrane topology and are retained in the endoplasmic reticulum (ER) by a membrane retention signal at their NH 2 termini. The FUT10-479 has, in addition, a COOH-ER membrane retention signal. The FUT10-391 is a soluble protein without a transmembrane domain or ER retention signal that transiently localizes to the Golgi and then is routed to the lysosome. After transfection in COS7 cells, the three FUT10s and at least one FUT11, link ␣-L-fucose onto conalbumin glycopeptides and biantennary N-glycan acceptors but not onto short lactosaminyl acceptor substrates as do classical monoexonic ␣1,3-fucosyltransferases. Modifications of the innermost core GlcNAc of the N-glycan, by substitution with ManNAc or with an opened GlcNAc ring or by the addition of an ␣1,6-fucose, suggest that the FUT10 transfer is performed on the innermost GlcNAc of the core chitobiose. We can exclude ␣1,3-fucosylation of the two peripheral GlcNAcs linked to the trimannosyl core of the acceptor, because the FUT10 fucosylated biantennary N-glycan product loses both terminal GlcNAc residues after digestion with human placenta ␣-N-acetylglucosaminidase.

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Activity, Splice Variants, Conserved Peptide Motifs, and Phylogeny of Two New α1,3-Fucosyltransferase Families (FUT10 and FUT11)

Mollicone

Moore

Bovin

et al. 2009

Journal of Biological Chemistry

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“…Besides the mutations described, probably a DNA rearrangement at the SEC1 locus occurred during and/or after a retrotransposition event in humans. It is widely known that subsequent mutations are facilitated by the previous gene-inactivating rearrangement (Saunier et al, 2001).…”

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confidence: 99%

“…Although differing in genomic organization and specificities of encoding enzymes, all α1,2 FUT share some characteristics, including i) a monoexonic coding sequence, ii) a complex tissue-and cell type-specific expression pattern regulated by several 5' untranslated exons, and iii) encode an enzyme that catalyses the final step in fucosylated glycan synthesis (Costache et al, 1997;Saunier et al, 2001;Javaud et al, 2003).…”

Section: Introductionmentioning

confidence: 99%

“…Conversely, SEC1 is inactive due to the deletion of two nucleotides, resulting in a truncate polypeptide with 246 amino acids. This gene also presents some characteristics of a non-autonomous retrotransposon, such as association with Alu-like elements and presence of a poly-A tail (Kelly et al, 1995;Saunier et al, 2001). On the other hand, Hitoshi et al (1995) and Barreaud et al (2000) showed that these three genes are fully activated in bovines and rabbits.…”

Section: Introductionmentioning

confidence: 99%

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Evolution of the SEC1 gene in New World monkey lineages (Primates, Platyrrhini)

Borges¹,

Paiva²,

Harada³

2008

Genet. Mol. Res.

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ABSTRACT. The structure and evolution of the SEC1 gene were examined for the first time in New World primates of the genera Alouatta, Aotus, Ateles, Brachyteles, Callicebus, Callithrix, Cebus, Chiropotes, Lagothrix, Leontopithecus, Pithecia, Saguinus, and Saimiri. This gene has a high CG content (63.8%) and an estimated heterogeneous size ranging from 795 (Callithrix) to 1041 bp (Pithecia), due to numerous indel events. Similar to other fucosyltransferases, three conserved regions are shared by these primates, except for the callitrichines, Aotus and Pithecia, in which indel events resulted in premature stop codons that are related to the production of a supposedly non-functional protein. Phylogenetic analysis of the SEC1 gene, transition/transversion rates, and nucleotide sequence alignment support the hypothesis that primate SEC1 evolved by divergent evolution, and that the lack of activity in some lineages occurred independently at least twice in New World primates, once in the Aotus-Cebus-Callitrichinae group and again in Pithecia. Likelihood-based inference of ancestral states for the activity of SEC1 leads us to suppose that inactivation of SEC1 in the Callitrichinae was a result of a more complex series of events than in Pithecia.

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“…The lack of intron sequences in most of the genes of vertebrate ·2-fucosyltransferases (Saunier et al, 2001) and vertebrate ·3/4-fucosyltransferases (Wierinckx et al, 1999), suggests that two independent retro-transposition events of processed mRNA, in each of these two families, has led to the loss of intron sequences, and the present vertebrate enzymes with mono-exon coding sequences (Javaud et al, 2003). The other orthologous fucosyltransferase genes in invertebrates and plants as well as the ·6-fucosyltransferases and the protein-Ofucosyltransferases have poly-exon structures as the majority of eukaryote genes.…”

Section: Fut8mentioning

confidence: 99%

Cytogenetics, conserved synteny and evolution of chicken fucosyltransferase genes compared to human

Coullin

Crooijmans²,

Fillon³

et al. 2003

Cytogenet Genome Res

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Fucosyltransferases appeared early in evolution, since they are present from bacteria to primates and the genes are well conserved. The aim of this work was to study these genes in the bird group, which is particularly attractive for the comprehension of the evolution of the vertebrate genome. Twelve fucosyltransferase genes have been identified in man. The orthologues of theses genes were looked for in the chicken genome and cytogenetically localized by FISH. Three families of fucosyltransferases: α6-fucosyltransferases, α3/4-fucosyltransferases, and protein-O-fucosyltransferases, were identified in the chicken with their associated genes. The α2-fucosyltransferase family, although present in some invertebrates and amphibians was not found in birds. This absence, also observed in Drosophila, may correspond to a loss of these genes by negative selection. Of the eight chicken genes assigned, six fell on chromosome segments where conservation of synteny between human and chicken was already described. For the two remaining loci, FUT9 and FUT3/5/6, the location may correspond to a new small syntenic area or to an insertion. FUT4 and FUT3/5/6 were found on the same chicken chromosome. These results suggest a duplication of an ancestral gene, initially present on the same chromosome before separation during evolution. By extension, the results are in favour of a common ancestor for the α3-fucosyltransferase and the α4-fucosyltransferase activities. These observations suggest a general mechanism for the evolution of fucosyltransferase genes in vertebrates by duplication followed by divergent evolution.

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Organization of the Bovine α2-Fucosyltransferase Gene Cluster Suggests that the Sec1 Gene Might Have Been Shaped Through a Nonautonomous L1-Retrotransposition Event Within the Same Locus

Cited by 9 publications

References 38 publications

Activity, Splice Variants, Conserved Peptide Motifs, and Phylogeny of Two New α1,3-Fucosyltransferase Families (FUT10 and FUT11)

Activity, Splice Variants, Conserved Peptide Motifs, and Phylogeny of Two New α1,3-Fucosyltransferase Families (FUT10 and FUT11)

Evolution of the SEC1 gene in New World monkey lineages (Primates, Platyrrhini)

Cytogenetics, conserved synteny and evolution of chicken fucosyltransferase genes compared to human

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