Organizational culture is the combination of the beliefs, values, behavior norms, formal and informal system processes and methods. As of 1980, the organizational culture, which is an important phenomenon in business management, has become a dominant issue in defining the behaviors of the employees in tourism businesses. Organizational culture in hotel businesses ensures that the employees are motivated towards acting against uncertainties with a mutual purpose, and that they move in a safe path. In this study, the 5-star hotels in Istanbul have been evaluated in terms of organizational culture types (Adhocracy, Klan, Bureaucracy and Market) set by Cameron and Quinn "Competing Values Model". In the study context, 335 employees replied to questionnaires in 5-star hotels. According to the results of the study, the findings suggested that the mean values of the organizational culture types are higher; however, there are no clear differences among culture types.