This research aims to expand the conceptualization of the individual and organizational characteristics which are associated with organizational Entrepreneurial Orientation (EO) in public administration. It also attempts to provide organizations and their administrators, public officers, strategy and public policy makers, with a strong framework, supported by valuable information, on how to create an effective working environment which encourages the development of EO behavior. A theoretical framework and a theoretical methodological tool were designed in order to demonstrate such relations. By capturing the employee perceptions on attitudes, behavior and practices in their workplace, it is examined whether a public organization is innovative and entrepreneurial oriented. Knowing more about the observed variables, this research will provide information which would reveal the organizational and the individual characteristics which will support, promote and amplify the ΕΟ within the working environment of public organizations.