Organo-modified bentonite for gentamicin topical application: Interlayer structure and in vivo skin permeation

Iannuccelli, Valentina; Maretti, Eleonora; Bellini, Alessia; Malferrari, Daniele; Ori, Guido; Montorsi, Monia; Bondi, Moreno; Truzzi, Eleonora; Leo, Eliana Grazia

doi:10.1016/j.clay.2018.03.029

Cited by 23 publications

(17 citation statements)

References 74 publications

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“…Gentamicin has been associated with several layered silicates from kaolinite (Hui et al, 2019) to smectites (Rapacz-Kmita et al, 2015, 2017a, 2017bIannuccelli et al, 2018;Jeong et al, 2018), and has also been incorporated in halloysite nanotubes (Rapacz-Kmita et al, 2019) and layered double hydroxides (Carja et al, 2007). Most of these studies analyzed the release of the antibiotic from the support and provided results on their antibacterial properties against various pathogens such as E. coli, S. aureus, or S. epidermidis.…”

Section: Introductionmentioning

confidence: 99%

Gentamicin-Montmorillonite Intercalation Compounds as an Active Component of Hydroxypropylmethylcellulose Bionanocomposite Films with Antimicrobial Properties

Darder

Charlet

et al. 2021

Clays and clay miner.

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The present study introduces an overview of gentamicin-clay mineral systems for applications in biomedicine and then focuses on the development of a series of gentamicin/clay hybrid materials to be used as the bioactive phase of hydroxypropylmethylcellulose (HPMC) to produce bionanocomposite membranes possessing antimicrobial activity of interest in wound-dressing applications. Gentamicin (Gt) was adsorbed from aqueous solutions into a montmorillonite (Cloisite®-Na+) to produce intercalation compounds with tunable content of the antibiotic. The hybrids were characterized by CHN chemical analysis, energy-dispersive X-ray analysis, X-ray diffraction, Fourier-transform infrared spectroscopy, and thermogravimetric analysis, confirming the intercalation of Gt by an ion-exchange mechanism. The release of Gt from the hybrids was tested in water and in buffer solution to check their stability. Hybrids with various amounts of Gt were incorporated into a HPMC matrix at various loadings and processed as films by the casting method. The resulting Gt-clay/HPMC bionanocomposites were characterized by means of field-emission scanning electron microscopy, and were also evaluated for their water-adsorption and mechanical properties to confirm their suitability for wound-dressing applications. The antimicrobial activity of the bionanocomposite films was tested in vitro toward various microorganisms (Escherichia coli, Pseudomonas aeruginosa, Staphylococcus aureus, methicillin-resistant Staphylococcus aureus (MRSA), vancomycin-resistant Enterococcus faecium, Acinetobacter baumannii, and Klebsiella pneumonia), showing a complete bacterial reduction even in films with small Gt contents.

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Section: Introductionmentioning

confidence: 99%

Gentamicin-Montmorillonite Intercalation Compounds as an Active Component of Hydroxypropylmethylcellulose Bionanocomposite Films with Antimicrobial Properties

Darder

Charlet

et al. 2021

Clays and clay miner.

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“…If acting directly on skin, it will be difficult for GT to penetrate skin to deeper layers. Its systemic absorption is low probably due to its cationic nature, and therefore its efficacy mainly stems from a topical effect of the superficial layer of skin [25]. Nonetheless, GT can induce renal tubular necrosis and renal tubular congestion [26] and kill intra-auricular lymphocytes [27] to trigger certain nephrotoxicity and ototoxicity and is therefore limited in clinical application to some extent.…”

Section: Introductionmentioning

confidence: 99%

Chitosan-Gentamicin Conjugate Hydrogel Promoting Skin Scald Repair

et al. 2020

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Our earlier research indicated that chitosan-gentamicin conjugate (CS-GT) possesses superior antimicrobial activity and good water solubility. To develop CS-GT-based scald dressings, the antibacterial properties of CS-GT were further studied, and the biosafety of CS-GT and the healing mechanism of CS-GT hydrogel was systematically explored in this article. It was found that cell viability shows a declined inclination with the prolonged culture time and the increased concentration of CS-GT. After three day’s culture, the cell viability could still remain at 79.72% when CS-GT concentration was as high as 1000 μg/mL. On the other hand, the hemolysis rate of CS-GT was lower than 5% when its concentration is 800 μg/mL. Therefore CS-GT has good cytocompatibility and hemocompatibility. A wound-healing experiment has shown that the skin healing rate of CS-GT hydrogel was the highest at 99.61%, followed by the positive control (wet burn ointment) 94.98%, GT hydrogel 87.50%, and matrix 77.39%. The blank control group, however, possessed the lowest healing rate of 75.45%. Further analysis indicated that CS-GT hydrogel could promote the synthesis of total protein (TP) in skin granulation tissue, resulting in the enhanced hydroxyproline (HYP) content, which facilitated collagen fibrogenesis, reduced cytokine expression in an inflammatory response, and, ultimately, accelerated wound healing. To sum up, CS-GT hydrogel is a promising scald dressing.

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“…In this context, Iannuccelli et al [68] developed a novel system based on organo-modified bentonite, for the topical delivery of gentamicin, which may represent a safer alternative and more effective way of gentamicin utilization. This drug possesses a low systemic absorption, probably due to the difficult penetration through the deep layers of the skin, and therefore its use is restricted to the local effect that involves mainly the most superficial skin layers.…”

Section: Montmorillonitementioning

confidence: 99%

The Use of Some Clay Minerals as Natural Resources for Drug Carrier Applications

Massaro

Colletti

Lazzara

et al. 2018

JFB

117

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The goal of modern research is to use environmentally preferable materials. In this context, clay minerals are emerging candidates for their bio- and ecocompatibility, low cost and natural availability. Clay minerals present different morphologies according to their layer arrangements. The use of clay minerals, especially in biomedical applications is known from ancient times and they are regaining attention in recent years. The most representative clay minerals are kaolinit, montmorillonite, sepiolites and halloysite. This review summarizes some clay minerals and their derivatives for application as nanocontainer for biologically active species.

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Organo-modified bentonite for gentamicin topical application: Interlayer structure and in vivo skin permeation

Cited by 23 publications

References 74 publications

Gentamicin-Montmorillonite Intercalation Compounds as an Active Component of Hydroxypropylmethylcellulose Bionanocomposite Films with Antimicrobial Properties

Gentamicin-Montmorillonite Intercalation Compounds as an Active Component of Hydroxypropylmethylcellulose Bionanocomposite Films with Antimicrobial Properties

Chitosan-Gentamicin Conjugate Hydrogel Promoting Skin Scald Repair

The Use of Some Clay Minerals as Natural Resources for Drug Carrier Applications

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