Organochlorine pesticides have been used extensively especially in tropical countries in malaria control programs as well as control of agricultural pests. However, much of the food we eat contain pesticide residues. Analysis of organochlorine compounds are carried out because of their potential health hazardous effect on humans. In this study, milk was monitored using HPLC, for six organochlorine pesticide residues (DDT, dielderin, endrin, heptachlor, heptachlor epoxide and lindane) to determine the degree of environmental contamination with them. The study included 40 samples of fresh dairy cow milk which were collected from some villages at Qena and Sohag Governorates (20 from Qena and 20 from Sohag) in the period from June 2002 to December 2002. The results of the study revealed that 82.5% were contaminated with one or more of the investigated organochlorine pesticides. The distribution was : (95%) of the samples from Qena and (70%) from Sohag. DDT complex was the most frequent contaminant. It was found in 30% of milk samples followed by lindane and heptachlor as each of them was found in 22.5% of milk samples. Endrin was found in 17.5% and then dieldrin and heptachlor epoxide as each was found in 15% of milk samples. The mean concentration of DDT complex was 0.1003 ppm 0.19. The mean concentration of heptachlor was 0.0575 ppm 0.013. Heptachlor epoxide was found in a mean concentration of 0.053 ppm 0.01, while the mean concentration of dieldrine was 0.1273 ppm 0.02. On comparison of the EDI (Estimated Daily Intake) in this study with the ADI (Average Daily Intake) data recommended by the Food and Agricultural Organization/ World Health Organization (FAO/WHO 1993), It was found that None of these values exceed the recommended values. The results of this work indicated that in spite of the banning much of organochlorine pesticides still contaminating the environment resulting in contamination of food stuffs, particularly milk. There is a potential risk of the consumption of such contaminated milk on human's health particularly infants and children. INTRODUCTION: Milk is at risk of being contaminated by a wide variety of chemical residues introduced via treatment of the cow, its feed, the milking environment and the processing plant. The potential chemical contaminants are: Antibiotics, Hormones, Disinfectants, Nitrites,