Organogenesis in leafy spurge (Euphorbia esula L.)

Davis, David W.; Olson, Prudence A.

doi:10.1007/bf02632278

Cited by 3 publications

(4 citation statements)

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“…Spermine was undetectable in leafy spurge hypocotyl segments prior to initiation of organs but the levels increased up to at least 14 days. This is the most active period of organogenesis in the isolated leafy spurge hypocotyl segments (Davis and Olson 1993 and the present study). However, as with putrescine, the greatest increases of both spermidine and spermine were obtained in tissues treated with 450 nM 2,4-D, which strongly inhibited both roots and shoots.…”

Section: Discussionsupporting

confidence: 67%

“…All parts of etiolated, aseptically hibition of root formation was reversed by the addition germinated seedlings are capable of regenerating new of putrescine, whereas the DFMO-induced inhibition of plants, and hypocotyl segments have been used to deter-root formation was not (Davis and Olson 1994). mine the effects of auxins and cytokinins on organ for-The effects of DFMO on different plant systems are mation (Davis and Olson 1993).…”

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confidence: 99%

“…1988, Evenson et al 1988). In leafy spurge hypocotyl Nutrients for the B5 medium were plant tissue culture segments, 230 nM 2,4-D inhibited root and shoot forma-tested by Sigma Chemical Co. Polyamines were obtion with no stimulation of root formation at any concen-tained from Sigma, or Aldrich Chemical Co., Inc. (Miltration (Davis and Olson 1993). In this way 2,4-D af-waukee, WI, USA).…”

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2,4‐Dichlorophenoxy acetic acid and indoleacetic acid partially counteract inhibition of organogenesis by difluoromethylornithine

Davis¹

1997

Physiologia Plantarum

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Abstract:Difluoromethylornithine (DFMO) is a well known inhibitor of putrescine biosynthesis that has been reported to interact in varying ways with auxins such as indoleacetic acid (IAA) and 2,4-dichlorophenoxy acetic acid (2,4-D). In the present report DFMO is shown to inhibit root formation in isolated hypocotyl segments of Euphorbia esula L. (leafy spurge) grown in the dark on solidified nutrient media in Petri dishes. Shoot formation was only slightly inhibited by DFMO and only on media with salts and vitamins diluted 10-fold. 2,4-D inhibited both root and shoot formation in full strength or diluted media. Simultaneous application of both compounds resulted in partial reversal of root inhibition, but only at 450 nM 2,4-D, the highest concentration used. In both media IAA also partially reversed DFMO effects on root formation. The effects of DFMO, 2,4-D or IAA on root (or shoot) formation do not appear to be closely related to endogenous content of the polyamines determined by high performance liquid chromatography. Abbreviations:DFMA, DL-α-difluoromethylarginine; DFMO, DL-α-difluoromethy-

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Section: Discussionsupporting

confidence: 67%

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confidence: 99%

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confidence: 99%

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2,4‐Dichlorophenoxy acetic acid and indoleacetic acid partially counteract inhibition of organogenesis by difluoromethylornithine

Davis¹

1997

Physiologia Plantarum

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“…All parts of etiolated aseptically germinated leafy spurge seedlings are capable of regenerating new plants (Davis and Olson, 1993). Hypocotyl segments were used to determine the effects of auxins and cytokinins on the formation of visible roots and shoots, which occurs approximately seven to 10 days after excision, with almost no callus formation in control tissues (Davis and Olson, 1993). The polyamines putrescine, spermidine and spermine are involved in plant cell division and development (Slocum and Flores, 1991;Galston and Kaur-Sawhney, 1995).…”

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confidence: 99%

Polyamines, auxins and organogenesis in leafy spurge (Euphorbia esula L.)

Davis¹

1997

Journal of Plant Physiology

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Summary:Canaline and canavanine (inhibitors of polyamine biosynthesis) effects on root and shoot formation were determined in vitro for etiolated hypocotyl segments of leafy spurge (Euphorbia esula L.). Canaline (an ornithine analogue) at 10 to 100 µmol/L inhibited root and shoot formation. The simultaneous application of putrescine did not reverse canaline-induced inhibition of root formation. Ornithine, a precursor of putrescine via the ornithine decarboxylase pathway, unexpectedly inhibited root formation. Canavanine (an arginine analogue) at 30 to 100 µmol/L inhibited root and/or shoot formation. Inhibition of root formation by canavanine was not reversed by agmatine or putrescine, but was partially reversed by arginine. Indoleacetic acid (IAA) inhibited shoot formation, stimulated root formation, and partially reversed the inhibition of root formation by either canaline or canavanine. IAA generally increased endogenous levels of free polyamines in controls and canaline-or canavanine-treated tissues, but these changes were small and probably had little effect on organogenesis. IAA also partially reversed the inhibition of root (but not shoot) formation by arcaine and pentamidine, inhibitors of polyamine action in mammals. The roles of the polyamines in plants is not clear, but the polyamines may play only minor roles in root and shoot formation.

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Effects of putrescine and inhibitors of putrescine biosynthesis on organogenesis inEuphorbia esula L.

Davis

Olson

1994

In Vitro Cell Dev Biol - Plant

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Organogenesis in leafy spurge (Euphorbia esula L.)

Cited by 3 publications

References 19 publications

2,4‐Dichlorophenoxy acetic acid and indoleacetic acid partially counteract inhibition of organogenesis by difluoromethylornithine

2,4‐Dichlorophenoxy acetic acid and indoleacetic acid partially counteract inhibition of organogenesis by difluoromethylornithine

Polyamines, auxins and organogenesis in leafy spurge (Euphorbia esula L.)

Effects of putrescine and inhibitors of putrescine biosynthesis on organogenesis inEuphorbia esula L.

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