Organogenesis responses of tea (Camellia sinensis (L.) O. Kuntze) var. assamica and sinensis

Widhianata, Hani; Taryono, Taryono

doi:10.1063/1.5098431

Cited by 5 publications

(2 citation statements)

References 6 publications

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“…In contrast, [7] used full MS media and 3/4 and 1/2 MS media with the addition of BA and Kinetin. In addition to coffee plants, shoot multiplication techniques have also been applied to other significant plant species, including tea [8], rosa [9], banana [10], sugarcane [11], stevia [12], macadamia [13] and porang [14,15]. However, very few have researched the effect of using apical and axillary shoots in the propagation process, including coffee plants.…”

Section: Introductionmentioning

confidence: 99%

Multiplication of apical and axillary shoots of coffee arabica using various types of cytokinins

Ibrahim,

Sari,

Noorrohmah

et al. 2024

IOP Conf. Ser.: Earth Environ. Sci.

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In-vitro shoot multiplication presents a promising avenue for replicating the genetic characteristics of parent plants, commonly utilized in various plant biotechnological techniques such as mutation replication, protoplast fusion, anther culture, and genetic engineering. The primary aim of this research was to assess the impact of different cytokinins on the proliferation of apical and axillary shoots in Arabica coffee multiplication. A Completely Randomized Factorial Design was employed, considering two factors: explant source and cytokinin type. Explants were sourced from both apical and axillary branches of AS2K Arabica coffee plantlets. Three cytokinins, namely 6-Benzyl adenine purine (BAP), isopentenyl adenine (2-IP), and kinetin, were incorporated into the Murashige and Skoog (MS) 1962 basal medium at concentrations of 0, 1, 2, and 3 mg/l. The findings revealed significant effects of all three cytokinins on shoot number, plantlet height, and leaf growth, albeit without influencing root development. Notably, axillary shoot-derived explants demonstrated a more pronounced effect on shoot count compared to those from apical shoots. Among the cytokinin treatments assessed, BAP at a concentration of 2 mg/l in the MS medium yielded optimal results for propagating the AS2K variety of Arabica coffee, thus representing the recommended medium for this purpose.

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Section: Introductionmentioning

confidence: 99%

Multiplication of apical and axillary shoots of coffee arabica using various types of cytokinins

Ibrahim,

Sari,

Noorrohmah

et al. 2024

IOP Conf. Ser.: Earth Environ. Sci.

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“…BAP deficiency was reported to be associated with a decline in the ability to initiate shooting (Werner et al 2008). In tea's tissue culture, the use of BAP has been widely reported, such as using shoot tips and axillary buds of TRI2025 from the field as explants (Widhianata & Taryono 2019); with leaf explants of TRI2024 and TRI2025 (Farida & Muslihatin 2017); also cotyledon explants of TRI2025, Malabar2, Cinyuruan 143, and Kiara8 clone (Putra et al 2015).…”

Section: Introductionmentioning

confidence: 99%

Morphological, Histological, and Protein Profiling of Tea Embryo Axis at Early Stage of Culture

Eskundari

Taryono

Indradewa

et al. 2021

J.Tropical Biodiversity Biotechnology

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Tissue culture is an alternative choice of plant propagation either through somatic embryogenesis or in vitro organogenesis techniques. TRI2025 tea clone has been cultured successfully, however, the scientific information related to morphology, histology, and protein profile at an early event of culturing time has not been reported yet. This study aimed to determine the differences between those pathways, in the context of morphology, histology, and protein profile. The explants were the embryo axis of TRI2025 tea clone cultured on two different induction mediums; somatic embryogenesis and in vitro organogenesis induction medium. The results showed that most of the explants cultured on A medium developed to be a globular-like structure at 11-day after culture (DAC), while all explants cultured on B medium showed the initiation stage of in vitro organogenesis. Histological analysis showed meristem reconstruction at shoot apical meristem (SAM) and root apical meristem (RAM) at 11-DAC at explants cultured on B medium, while explants cultured on A medium showed callusing at 21-DAC. Protein profile analysis using SDS-PAGE showed protein bands of 54 and 81 KDa that only appeared at explants cultured on A medium start from 14-DAC, and those two protein bands thought to be a differentiator at the early stages of the two tissue culture techniques. Thus, these parameters can be used as early detection for plant tissue culture, especially in tea.

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Micropropagation

Mondal¹

2020

Tea: Genome and Genetics

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Organogenesis responses of tea (Camellia sinensis (L.) O. Kuntze) var. assamica and sinensis

Cited by 5 publications

References 6 publications

Multiplication of apical and axillary shoots of coffee arabica using various types of cytokinins

Multiplication of apical and axillary shoots of coffee arabica using various types of cytokinins

Morphological, Histological, and Protein Profiling of Tea Embryo Axis at Early Stage of Culture

Micropropagation

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