Organohalide-Respiring Bacteria as Members of Microbial Communities: Catabolic Food Webs and Biochemical Interactions

Richardson, Ruth

doi:10.1007/978-3-662-49875-0_14

Cited by 15 publications

(9 citation statements)

References 182 publications

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“…The interaction of complex bacterial communities appears to be essential for dechlorination in the environment, for example, through cross-feeding of electron donors, carbon sources, and cofactors. , Three dominant TRFs (166, 237, and 279) were found in the heavy DNA fractions of all three 13 C-acetate treatments with and without 1,2,3,4-TeCDD. These three TRFs presumably consumed acetate or its degradation products via cross-feeding for growth.…”

Section: Discussionmentioning

confidence: 96%

Identification of a Chlorodibenzo-p-dioxin Dechlorinating Dehalococcoides mccartyi by Stable Isotope Probing

Dam

Sun

McGuinness

et al. 2019

Environ. Sci. Technol.

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Polychlorinated dibenzo-p-dioxins (PCDDs) are released into the environment from a variety of both anthropogenic and natural sources. While highly chlorinated dibenzo-p-dioxins are persistent under oxic conditions, in anoxic environments, these organohalogens can be reductively dechlorinated to less chlorinated compounds that are then more amenable to subsequent aerobic degradation. Identifying the microorganisms responsible for dechlorination is an important step in developing bioremediation approaches. In this study, we demonstrated the use of a DNA-stable isotope probing (SIP) approach to identify the bacteria active in dechlorination of PCDDs in river sediments, with 1,2,3,4-tetrachlorodibenzo-p-dioxin (1,2,3,4-TeCDD) as a model. In addition, pyrosequencing of reverse transcribed 16S rRNA of TeCDD dechlorinating enrichment cultures was used to reveal active members of the bacterial community. A set of operational taxonomic units (OTUs) responded positively to the addition of 1,2,3,4-TeCDD in SIP microcosms assimilating 13C-acetate as the carbon source. Analysis of bacterial community profiles of the 13C labeled heavy DNA fraction revealed that an OTU corresponding to Dehalococcoides mccartyi accounted for a significantly greater abundance in cultures amended with 1,2,3,4-TeCDD than in cultures without 1,2,3,4-TeCDD. This implies the involvement of this Dehalococcoides mccartyi strain in the reductive dechlorination of 1,2,3,4-TeCDD and suggests the applicability of SIP for a robust assessment of the bioremediation potential of organohalogen contaminated sites.

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Section: Discussionmentioning

confidence: 96%

Identification of a Chlorodibenzo-p-dioxin Dechlorinating Dehalococcoides mccartyi by Stable Isotope Probing

Dam

Sun

McGuinness

et al. 2019

Environ. Sci. Technol.

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“…Jakobsen and Postma, 1999;Aulenta et al, 2008;Paul et al, 2016) with lower levels than predicted from thermodynamic considerations (Heimann et al, 2009). This phenomenon has been attributed to kinetic effects arising from the relative efficiencies in H 2 producers and consumers in microbial consortia (Richardson, 2016).…”

Section: Molecular Hydrogenmentioning

confidence: 97%

“…2) (Baker et al, 1999;Baker and Vervier, 2004;Hlaváčová et al, 2005). It is an easily assimilated carbon source and possible direct electron donor for OHRB which may be independent of extracellular H 2 (Richardson, 2016). Under high pore water H 2 concentrations (>350 nM) acetate is also produced from other labile DOC compounds (i.e.…”

Section: Short Chain Fatty Acidsmentioning

confidence: 99%

Natural attenuation of chlorinated ethenes in hyporheic zones: A review of key biogeochemical processes and in-situ transformation potential

et al. 2018

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Chlorinated ethenes (CEs) are legacy contaminants whose chemical footprint is expected to persist in aquifers around the world for many decades to come. These organohalides have been reported in river systems with concerning prevalence and are thought to be significant chemical stressors in urban water ecosystems. The aquifer-river interface (known as the hyporheic zone) is a critical pathway for CE discharge to surface water bodies in groundwater baseflow. This pore water system may represent a natural bioreactor where anoxic and oxic biotransformation process act in synergy to reduce or even eliminate contaminant fluxes to surface water. Here, we critically review current process understanding of anaerobic CE respiration in the competitive framework of hyporheic zone biogeochemical cycling fuelled by in-situ fermentation of natural organic matter. We conceptualise anoxic-oxic interface development for metabolic and co-metabolic mineralisation by a range of aerobic bacteria with a focus on vinyl chloride degradation pathways. The superimposition of microbial metabolic processes occurring in sediment biofilms and bulk solute transport delivering reactants produces a scale dependence in contaminant transformation rates. Process interpretation is often confounded by the natural geological heterogeneity typical of most riverbed environments. We discuss insights from recent field experience of CE plumes discharging to surface water and present a range of practical monitoring technologies which address this inherent complexity at different spatial scales. Future research must address key dynamics which link supply of limiting reactants, residence times and microbial ecophysiology to better understand the natural attenuation capacity of hyporheic systems.

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“…Several studies on dechlorinating communities containing D. mccartyi in association with fermenting, acetogenic and/or methanogenic bacteria revealed higher dechlorination and growth rates than those of pure cultures [16,18,19,20,21,22,23,24,25,26]. It is hypothesized that cross-feeding and a constant supply of growth factors such as corrinoids and biotin enhance growth [27]. Within these communities, non-dechlorinating fermenting bacteria provide hydrogen, acetate and CO 2 from e.g.…”

Section: Introductionmentioning

confidence: 99%

Interspecies metabolite transfer and aggregate formation in a co-culture of Dehalococcoides and Sulfurospirillum dehalogenating tetrachloroethene to ethene

Kruse

Türkowsky

Birkigt

et al. 2019

Preprint

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Microbial communities involving dehalogenating bacteria assist in bioremediation of areas contaminated with chlorinated hydrocarbons. To understand molecular interactions between dehalogenating bacteria, we co-cultured two bacterial species dechlorinating chloroethenes: Sulfurospirillum multivorans, dechlorinating tetrachloroethene (PCE) to cis-1,2-dichloroethene (cDCE) and Dehaloccoides mccartyi strains BTF08 or 195, transforming PCE via cDCE to ethene. The interaction of these bacteria cultivated with lactate as electron donor and PCE as electron acceptor was investigated using growth studies, metabolite analysis, microscopy, isotope fractionation and proteomics. Co-cultures exhibited more than 3-fold higher PCE to ethene dechlorination rates than D. mccartyi pure cultures. S. multivorans provided hydrogen, acetate and the reductive dehalogenase cobamide cofactor to D. mccartyi. While D. mccartyi 195 dechlorinated cDCE in the presence of norpseudo-B12 produced by S. multivorans, cDCE dechlorination by D. mccartyi BTF08 depended on the supply of 5,6-dimethylbenzimidazole for producing functional cobamides. Co-cultures were characterized by the formation of aggregates and electron microscopy revealed an extracellular matrix enabling cell-to-cell contact. D. mccartyi showed an unusual barrel-like morphology, probably dependent on down-regulation of cell division gene expression, as observed in the co-culture proteome. Only the reductive dehalogenases PteA and VcrA were found in the proteomes of D. mccartyi BTF08 during dehalogenation of PCE to ethene.

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Organohalide-Respiring Bacteria as Members of Microbial Communities: Catabolic Food Webs and Biochemical Interactions

Cited by 15 publications

References 182 publications

Identification of a Chlorodibenzo-p-dioxin Dechlorinating Dehalococcoides mccartyi by Stable Isotope Probing

Identification of a Chlorodibenzo-p-dioxin Dechlorinating Dehalococcoides mccartyi by Stable Isotope Probing

Natural attenuation of chlorinated ethenes in hyporheic zones: A review of key biogeochemical processes and in-situ transformation potential

Interspecies metabolite transfer and aggregate formation in a co-culture of Dehalococcoides and Sulfurospirillum dehalogenating tetrachloroethene to ethene

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