Organoids capture tissue-specific innate lymphoid cell development in mice and humans

Jowett, Geraldine M.; Read, Emily; Roberts, Luke B.; Coman, Diana; González, Marta Vilà; Zabinski, Tomasz; Niazi, Umar; Reis, Rita Antunes Dos; Trieu, Tung-Jui; Danovi, Davide; Gentleman, Eileen; Vallier, Ludovic; Curtis, Michael A.; Lord, Graham M.; Neves, Joana F.

doi:10.1016/j.celrep.2022.111281

Cited by 14 publications

(11 citation statements)

References 95 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Notably, epithelial intestinal or pulmonary cells were shown to mature co-cultured innate lymphoid cells into tissue-specific phenotypes without requiring subset-specific cytokine supplementation. 66 Complex organoid assembly may be cumbersome because of limitations related to the isolation of sufficient syngeneic cell types from human liver donor tissue or biopsy material. Using HLA-matched peripheral blood mononuclear cells from commercial or academic repositories or allogenic cell types for HLA-independent hypotheses may overcome the limitation.…”

Section: Primary Tissue-derived Organoids For Nafld Studiesmentioning

confidence: 99%

Modeling Nonalcoholic Fatty Liver Disease in the Dish Using Human-Specific Platforms: Strategies and Limitations

Rezvani¹,

Vallier²,

Guillot³

2023

Cellular and Molecular Gastroenterology and Hepatology

Self Cite

View full text Add to dashboard Cite

Section: Primary Tissue-derived Organoids For Nafld Studiesmentioning

confidence: 99%

Modeling Nonalcoholic Fatty Liver Disease in the Dish Using Human-Specific Platforms: Strategies and Limitations

Rezvani¹,

Vallier²,

Guillot³

2023

Cellular and Molecular Gastroenterology and Hepatology

Self Cite

View full text Add to dashboard Cite

“…In cocultures, both iPSCderived intestinal and lung organoids can also signal to ILC progenitors to drive tissue-specific maturation of the immune cell populations. [178] DCs are also important players in the intestine, which researchers have attempted to incorporate into tissue models. DCs interact with the IE, presenting antigens transcytosed by the IE to T cells to mount adaptive immune responses.…”

Section: Immune Cell Populationsmentioning

confidence: 99%

Listen to Your Gut: Key Concepts for Bioengineering Advanced Models of the Intestine

Cameron,

Neves,

Gentleman

2023

Advanced Science

Self Cite

View full text Add to dashboard Cite

The intestine performs functions central to human health by breaking down food and absorbing nutrients while maintaining a selective barrier against the intestinal microbiome. Key to this barrier function are the combined efforts of lumen‐lining specialized intestinal epithelial cells, and the supportive underlying immune cell‐rich stromal tissue. The discovery that the intestinal epithelium can be reproduced in vitro as intestinal organoids introduced a new way to understand intestinal development, homeostasis, and disease. However, organoids reflect the intestinal epithelium in isolation whereas the underlying tissue also contains myriad cell types and impressive chemical and structural complexity. This review dissects the cellular and matrix components of the intestine and discusses strategies to replicate them in vitro using principles drawing from bottom‐up biological self‐organization and top‐down bioengineering. It also covers the cellular, biochemical and biophysical features of the intestinal microenvironment and how these can be replicated in vitro by combining strategies from organoid biology with materials science. Particularly accessible chemistries that mimic the native extracellular matrix are discussed, and bioengineering approaches that aim to overcome limitations in modelling the intestine are critically evaluated. Finally, the review considers how further advances may extend the applications of intestinal models and their suitability for clinical therapies.

show abstract

“…The long time needed to obtain patient-derived PSCs is also a downside and may prevent the translation of such approaches to the clinic. [129] Primary Immune Cells: Many intestinal in vitro models incorporate primary immune cell subpopulations, including lymphocytes, [130][131][132][133][134] monocytes/macrophages, [135,136] dendritic cells, [137] and neutrophils. [138,139] Tissue-resident immune cells can be harvested from the intestinal lamina propria and show tissue-specific characteristics and functions, being phenotypically different from the ones found in other tissues or in circulation and are thus more physiologically relevant.…”

Section: Primary Cellsmentioning

confidence: 99%

“…This bidirectional relationship can be studied by coculturing immune cells with the epithelium. In this perspective, and due to performing distinct and specific functions in inflammation, different subsets of immune cells have been used in combination with organoids models: monocytes, [137] macrophages, [136,212] polymorphonuclear leukocytes, [138,139] lymphocytes, [130] and DCs. [141] Another drawback of organoids lies in their heterogeneous structures with a closed sphere-like spatial arrangement that prevents direct access to the apical side of the epithelium.…”

Section: Organoidsmentioning

confidence: 99%

Recent Advances in Cell‐Based In Vitro Models to Recreate Human Intestinal Inflammation

Macedo,

Dias Neto,

Pastrana

et al. 2023

Advanced Science

View full text Add to dashboard Cite

Inflammatory bowel disease causes a major burden to patients and healthcare systems, raising the need to develop effective therapies. Technological advances in cell culture, allied with ethical issues, have propelled in vitro models as essential tools to study disease aetiology, its progression, and possible therapies. Several cell‐based in vitro models of intestinal inflammation have been used, varying in their complexity and methodology to induce inflammation. Immortalized cell lines are extensively used due to their long‐term survival, in contrast to primary cultures that are short‐lived but patient‐specific. Recently, organoids and organ‐chips have demonstrated great potential by being physiologically more relevant. This review aims to shed light on the intricate nature of intestinal inflammation and cover recent works that report cell‐based in vitro models of human intestinal inflammation, encompassing diverse approaches and outcomes.

show abstract

Organoids capture tissue-specific innate lymphoid cell development in mice and humans

Cited by 14 publications

References 95 publications

Modeling Nonalcoholic Fatty Liver Disease in the Dish Using Human-Specific Platforms: Strategies and Limitations

Modeling Nonalcoholic Fatty Liver Disease in the Dish Using Human-Specific Platforms: Strategies and Limitations

Listen to Your Gut: Key Concepts for Bioengineering Advanced Models of the Intestine

Recent Advances in Cell‐Based In Vitro Models to Recreate Human Intestinal Inflammation

Contact Info

Product

Resources

About