Organoids in lung cancer: A teenager with infinite growth potential

Xu, Yiming; Xin, Wanghao; Yan, Chao; Shi, Yandong; Li, Yeping; Hu, Yanjie; Ying, Kejing

doi:10.1016/j.lungcan.2022.08.006

Cited by 11 publications

(5 citation statements)

References 73 publications

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“…Even with several passages, tumouroids have similar mutations to their original tumour [91], highlighting that these can be used as reliable preclinical models. A strong clonal dynamic in tumouroids has recently been described, leading to pre-existing minor subclones [92]. The inherent genomic instability of cancer cells, as in all other models, is likely to result in completely novel genetic alterations during the continuous propagation of organoid models.…”

Section: Advantagesmentioning

confidence: 99%

“…In Clevers' and Driehuis's methods, tumour samples were collected in Advanced Dulbecco's Modified Eagle Medium/Ham's F-12 (Advanced DMEM/F12) with L-alanyl-L-glutamine, which is a dipeptide substitute for L-glutamine (1× GlutaMAX), and included Penicillin-streptomycin, HEPES, and Primocin [62]. A recent study published by the same group recommended the addition of Rho kinase (ROCK) inhibitor (Y-27632), which helps tumour cells to proliferate in organoid cultures [92]. They also discourage sample transportation in sterilised ice-cold PBS, as it can result in cell death.…”

Section: Comparison Of Tumouroid Protocols Sample Collectionmentioning

confidence: 99%

“…They highlight the importance of maintaining the viability of the tumour sample (pink in colour) during the collection and transportation. Clevers' and Driehuis's method has been able to keep viable organoids for up to 72 h at 4 ˚C [92]. However, Kijima and Nakagawa recommend the transportation of samples in wet ice (4 ˚C).…”

Section: Comparison Of Tumouroid Protocols Sample Collectionmentioning

confidence: 99%

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Head and neck cancer patient-derived tumouroid cultures: opportunities and challenges

et al. 2023

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Head and neck cancers (HNC) are the seventh most prevalent cancer type globally. Despite their common categorisation, HNCs are a heterogeneous group of malignancies arising in various anatomical sites within the head and neck region. These cancers exhibit different clinical and biological manifestations, and this heterogeneity also contributes to the high rates of treatment failure and mortality. To evaluate patients who will respond to a particular treatment, there is a need to develop in vitro model systems that replicate in vivo tumour status. Among the methods developed, patient-derived cancer organoids, also known as tumouroids, recapitulate in vivo tumour characteristics including tumour architecture. Tumouroids have been used for general disease modelling and genetic instability studies in pan-cancer research. However, a limited number of studies have thus far been conducted using tumouroid-based drug screening. Studies have concluded that tumouroids can play an essential role in bringing precision medicine for highly heterogenous cancer types such as HNC.

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Section: Advantagesmentioning

confidence: 99%

Section: Comparison Of Tumouroid Protocols Sample Collectionmentioning

confidence: 99%

Section: Comparison Of Tumouroid Protocols Sample Collectionmentioning

confidence: 99%

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Head and neck cancer patient-derived tumouroid cultures: opportunities and challenges

et al. 2023

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“…( Figure 1 ) [ 117 ]. Lung organoids derived from patients due to their capability to recapitulate cancer tissue architecture and microenvironment are now being used to understand molecular complexities and patient-specific treatment modalities [ 118 , 119 ].…”

Section: Application Of Organoids In Cigarette-smoking-associated Lun...mentioning

confidence: 99%

Lung Organoids in Smoking Research: Current Advances and Future Promises

Agraval

Chu

2022

Biomolecules

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Tobacco smoking has been established to contribute to the pathogenesis of various respiratory diseases including chronic obstructive pulmonary disease (COPD), lung cancer, and asthma. However, major hurdles in mechanistic studies on the role of smoking in human lungs remain in part due to the lack of ex vivo experimental models and ambiguous data from animal models that can best recapitulate the architecture and pathophysiology of the human lung. Recent development of the lung organoid culture system has opened new avenues for respiratory disease research as organoids are proving to be a sophisticated ex vivo model that functionally and structurally mimics the human lungs better than other traditionally used models. This review will discuss how recent advances in lung organoid systems may help us better determine the injurious and immunological effect of smoking on human lungs and will provide some suggestions for future research directions.

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“…For some authors, organoid culture represents a better option for the study of lung cancer since it better reflects the epigenetic or histological peculiarities of this pathology. However, the possibility of generating organoids by choosing a certain composition should allow us to better study the role of each element that makes up the TME [ 16 , 17 ].…”

Section: Introductionmentioning

confidence: 99%

Morphological Characterization of Human Lung Cancer Organoids Cultured in Type I Collagen Hydrogels: A Histological Approach

Monleón-Guinot,

Milian,

Martínez-Vallejo

et al. 2023

IJMS

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The malignity of lung cancer is conditioned by the tumor microenvironment (TME), in which cancer-associated fibroblasts (CAFs) are relevant. In this work, we generated organoids by combining A549 cells with CAFs and normal fibroblasts (NF) isolated from adenocarcinoma tumors. We optimized the conditions for their manufacture in a short time. We evaluated the morphology of organoids using confocal microscopy analysis of F-actin, vimentin and pankeratin. We determined the ultrastructure of the cells in the organoids via transmission electron microscopy and the expression of CDH1, CDH2 and VIM via RT-PCR. The addition of stromal cells induces the self-organization of the organoids, which acquired a bowl morphology, as well as their growth and the generation of cell processes. They also influenced the expression of genes related to epithelial mesenchymal transition (EMT). CAFs potentiated these changes. All cells acquired a characteristic secretory phenotype, with cohesive cells appearing inside the organoids. In the periphery, many cells acquired a migratory phenotype, especially in organoids that incorporated CAFs. The deposit of abundant extracellular matrix could also be observed. The results presented here reinforce the role of CAFs in the progression of lung tumors and could lay the foundation for a useful in vitro pharmacological model.

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Organoids in lung cancer: A teenager with infinite growth potential

Cited by 11 publications

References 73 publications

Head and neck cancer patient-derived tumouroid cultures: opportunities and challenges

Head and neck cancer patient-derived tumouroid cultures: opportunities and challenges

Lung Organoids in Smoking Research: Current Advances and Future Promises

Morphological Characterization of Human Lung Cancer Organoids Cultured in Type I Collagen Hydrogels: A Histological Approach

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