Organometallic ruthenium(II) complexes containing NS donor Schiff bases: Synthesis, structure, electrochemistry, DNA/BSA binding, DNA cleavage, radical scavenging and antibacterial activities

We report the design, synthesis, and cytotoxic evaluation of a new organoruthenium(II) complex (1). Complex 1 was characterized by analytical and spectroscopic methods including single crystal X‐ray diffraction studies. Complex 1 crystallized in an orthorhombic crystal system with Pca21 space group with Ru(II) ion p‐cymene arene unit in a usual “piano‐stool” geometry. The DFT and TDFT studies were carried out to provide better insight about the spectral properties of the complex. The Hirshfeld surface analysis plots revealed significant intermolecular interactions within the crystal structure that essentially involve a network of N–H···H, O–H···O, and C–H···O linkages. DNA photocleavage activity revealed oxidative mechanistic pathway with preferential affinity toward minor groove. Cytotoxic studies of complex 1 via SRB assay revealed moderate but selective activity toward pancreatic and cervical cancer cell lines.

“…We have further calculated the apparent binding constant K app by using the equation. [ 38 ]

K_{app} \times ([], complex bold1) = K_{EB} bold\times ([], EB),

Section: Resultsmentioning

confidence: 99%

“…We have further calculated the apparent binding constant K app by using the equation. [38] K app × complex 1…”

Section: Resultsmentioning

confidence: 99%

Synthesis, structural investigations, and cytotoxic evaluation of a half‐sandwich Ru(II)‐arene complex

Yousuf

Bashir

2020

“…Form III is positioned between these two structures. [69] The electrophoresis results of the 200 mg/ml concentration of chiral dioxaborinane compounds after incubation with supercoiled plasmid pBR322 DNA are given in Figure 9. According to the results, a band (Form I) was obtained for the control untreated with compounds (Lane C) and two bands (Form I and II) from the samples treated with the compounds (Lane 1-10).…”

Section: Entrymentioning

confidence: 99%

Synthesis of cis‐1,2‐diol‐type chiral ligands and their dioxaborinane derivatives: Application for the asymmetric transfer hydrogenation of various ketones and biological evaluation

Kılıç

Balci

Arslan

et al. 2020

Two cis‐1,2‐diol‐type chiral ligands (T1 and T2) and their tri‐coordinated chiral dioxaborinane (T(1–2)B(1–2)) and four‐coordinated chiral dioxaborinane adducts with 4‐tert‐butyl pyridine sustained by N → B dative bonds (T(1–2)B(1–2)‐N) were synthesized and characterized by various spectroscopic techniques such as NMR (1H, 13C, and 11B), FT‐IR and UV–Vis spectroscopy, LC–MS/MS, and elemental analysis. It was suggested that both ferrocene and trifluoromethyl groups played key roles in the catalytic and biological studies because they could tune the solubility of the chiral dioxaborinane complexes and adjust the strength of intermolecular interactions. To assess the biological activities of newly synthesized chiral dioxaborinane compounds, DPPH (2,2‐diphenyl‐1‐picrylhydrazyl) radical scavenging, reducing power, antibacterial, DNA binding, and DNA cleavage activities were tested. Then, all chiral dioxaborinane complexes were investigated as catalysts for the asymmetric transfer hydrogenation of various ketones under suitable conditions. The results indicated that the chiral dioxaborinane catalysts performed well with high yields.

“…Human colon cancer cells (HT-29) and breast cancer cells (MCF-7) were grown on cover slips (1 × 10 5 cells/cover slip) incubated for 24 hr with various concentrations (5,10, and 20 μM) of complexes 1-4. They were then fixed in an ethanol:acetic acid solution (3:1, v/v).…”

Section: Cell Morphology Analysismentioning

confidence: 99%

New organoruthenium metallates containing ferrocenecarboxalidine thiosemicarbazones and their nucleic acid/albumin binding and in vitro cytotoxicity

Sindhu

Kalaivani

Prabhakaran

2020

Self Cite

A string of four new hetero binuclear Ru(III) complexes of ferrocenecarboxaldehyde-4(N)-substituted thiosemicarbazones were synthesized and characterized by various spectral (infrared, ultraviolet-visible, Electron Paramagnetic Resonance (EPR) and High Resolution Mass Spectrometry (HR-MS) techniques. The binding abilities of the ligands/complexes with nucleic acid (calf thymus DNA, CT-DNA) and bovine serum albumin (BSA) were analyzed by absorption and emission titration methods. The complexes exhibited better DNA binding affinity than their parent ligands. The interaction with CT-DNA was found to be intercalative and with BSA static quenching mechanism was observed. All the synthesized Ru(III) complexes were subjected to study their in vitro cytotoxicity against MCF-7 (human breast cancer) and HT-29 (human colon cancer) cell lines. Among the four complexes, complex 3 [RuCp (FF-etsc)PPh 3 ]Cl exhibited the highest cytotoxicity in MCF-7 cells and complex 4 [RuCp (FF-ptsc)PPh 3 ]Cl was the most active on HT-29 cells. K E Y W O R D S acridine orange-ethidium bromide (AO-EB)/4, 6-diamidino-2-phenylindole (DAPI) staining, morphological studies, MTT assay, Ru(III) complexes