Organotypic culture and surface plantation using umbilical cord epithelial cells: morphogenesis and expression of differentiation markers mimicking cutaneous epidermis

Mizoguchi, Masayuki; Suga, Yasushi; Sanmano, B; Ikeda, Shigaku; Ogawa, Hideoki

doi:10.1016/j.jdermsci.2004.06.003

Cited by 41 publications

(36 citation statements)

References 22 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Consistent with the previous reports suggesting the presence of both epithelial and mesenchymal stem cells in umbilical cords, [3][4][5] we also observed mesenchymal and epithelial cell colonies in our starting cultures of collagenase treated umbilical cord cell suspension. Mesenchymal colonies appeared earlier around 3-4 days after culture whereas epithelial colonies were evident in 7-8 days.…”

Section: Discussionsupporting

confidence: 93%

“…Two types of stem cells: epithelial and mesenchymal stem cells (MSCs) have been isolated and characterized from human umbilical cord. [3][4][5] Umbilical cord stem cells are multipotent, possess high proliferation and immunoregulatory properties. 6 Moreover, their collection is relatively easy and does not involve any invasive interventions.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Replication of influenza A virus in swine umbilical cord epithelial stem-like cells

Khatri

Chattha²

2014

Virulence

View full text Add to dashboard Cite

Section: Discussionsupporting

confidence: 93%

Section: Introductionmentioning

confidence: 99%

Replication of influenza A virus in swine umbilical cord epithelial stem-like cells

Khatri

Chattha²

2014

Virulence

View full text Add to dashboard Cite

“…The umbilical cord is delimited by a single layer of cubic epithelial cells forming the umbilical epithelium, an ectoderm derivative that continues with amniotic epithelial cells at the placental end and with the tegument epithelium of the foetus [6,7]. No structural borders are present between the distinct zones of UC, although it has been reported that Wharton's jelly features a relatively lower cell density than the other zones [8].…”

Section: Introductionmentioning

confidence: 99%

Novel Immunomodulatory Markers Expressed by Human WJ-MSC: an Updated Review in Regenerative and Reparative Medicine

Rocca¹,

Corrao

Iacono

et al. 2012

TOTERMJ

View full text Add to dashboard Cite

Mesenchymal (stromal) stem cells (MSC) are a broad class of stromal populations which are able to differentiate towards mature cell types, and do express molecules involved in immune modulation, tolerance induction and inflammation dampening. MSC can be virtually isolated from each adult organ, as well as from foetus-associated perinatal tissues. In particular, Wharton's jelly-derived MSC (WJ-MSC) bear all of these key properties, together with their ease of sourcing and lack of ethical issues.Cellular therapy is a key technique in regenerative medicine approaches, in particular for the treatment of diseases in which physiological processes of cellular repopulation are blocked by the underlying pathological conditions. Recent data enlightened the ability of administered cells to act also in a repopulation-independent fashion in target organs, since their peculiar immunomodulatory and anti-inflammatory features may favor organ self-repair by reactivating local progenitors by both cell-mediated or paracrine mechanisms. Translating classical regenerative medicine to "reparative medicine" or "support medicine" should represent a further therapeutic strategy independent from the differentiation capacity of MSC populations.Recent data further highlighted that WJ-MSC outperform BM-MSC (which are now being applied clinically) both in terms of immune modulation and lack of tumorigenesis (or tumor-promoting activities) in vivo. Starting from these premises, this paper analyzes the recent data on the biology of WJ-MSC, considering the role of both naïve and differentiated cells in immune modulation. In particular, the role of tolerance promoting pathways via non-classical B7 costimulators or class Ib MHC molecules are examined. In addition, we also analyzed the interconnections with other mechanicistic pathways (as those driven by matrix degrading metalloproteinases) in immune modulation. Our observations strongly support the notion that WJ-MSC may constitute a new tool in regenerative and reparative medicine applications.

show abstract

“…It is covered by the umbilical epithelium, called also amniotic epithelium, layered with cubic epithelial cells [1]. The inner structure of the umbilical cord is composed of umbilical vessels, two arteries and one vein, embedded in mucous connective tissue known as Wharton's jelly (WJ).…”

Section: Introductionmentioning

confidence: 99%

Perinatal and Wharton's Jelly-Derived Mesenchymal Stem Cells in Cartilage Regenerative Medicine and Tissue Engineering Strategies

Iacono

Anzalone

Corrao

et al. 2011

TOTERMJ

View full text Add to dashboard Cite

Stem cells can be found in embryonic and extraembryonic tissues as well as in adult organs. In particular, research in the last few years has delineated the key features of perinatal stem cells derived from fetus-associated tissues. These cells show multiple differentiation potential, can be easily expanded ex vivo, and raise no ethical concerns as regards their use. Several reports indicate that cells isolated from Wharton's jelly (WJ), the main component of umbilical cord extracellular matrix, are multipotent stem cells that express markers shared by other mesenchymal stem cells (MSC) and give rise to different mature cell types belonging to all three germ layers. Moreover, WJ-MSC display promising hypoimmunogenic and immunomodulatory properties, since they express molecules able to modulate NK cells and expand regulatory T-cell populations.In this review, we focus on the use of perinatal stem cells for regenerative medicine aimed at cartilage repair and regeneration. Cartilage is a specialized connective tissue which has poor regeneration and self-repair capacity in vivo. Traumatic injury or autoimmune processes are among the main causes of cartilage damage and degeneration, for which new hope comes from tissue engineering using stem cells which have undergone chondrocyte-like differentiation. We analyze the in vitro and in vivo data on the use of perinatal stem cells, in particular WJ-MSC, for cartilage regenerative medicine. The high variability of cell sources, the use of different types of scaffolds and matrixes, and the administration of several combinations of growth factors clearly point out the need for further research to optimize this cellular therapy approach and translate the results obtained from bench to clinic.

show abstract

Organotypic culture and surface plantation using umbilical cord epithelial cells: morphogenesis and expression of differentiation markers mimicking cutaneous epidermis

Cited by 41 publications

References 22 publications

Replication of influenza A virus in swine umbilical cord epithelial stem-like cells

Replication of influenza A virus in swine umbilical cord epithelial stem-like cells

Novel Immunomodulatory Markers Expressed by Human WJ-MSC: an Updated Review in Regenerative and Reparative Medicine

Perinatal and Wharton's Jelly-Derived Mesenchymal Stem Cells in Cartilage Regenerative Medicine and Tissue Engineering Strategies

Contact Info

Product

Resources

About