Oribatid mites in soil toxicity testing—the use ofOppia nitens(C.L. Koch) as a new test species

Abstract

Few soil invertebrate species are available for the toxic assessment of soils from boreal or other northern ecozones, yet these soils cover the majority of Canada's landmass as well as significant portions of Eurasia. Oppia nitens (C.L. Koch) is an herbivorous and fungivorous oribatid mite found in soil throughout Holarctic regions, including Canada. Soil tests using O. nitens were performed using 15 different forest soil types and horizons to investigate test variability in adult survival and reproduction. Adult survival (86.1 +/- 1.1%) was consistent across soil types, with a coefficient of variation (CV) of 15%. However, reproduction varied significantly, ranging from 2.9 (+/-1.1) to 86.2 (+/-11.7) individuals, with a corresponding CV of 118 and 30%, respectively. Of the soil factors assessed (NH(3), NO(3), pH, phosphorus [P], organic matter content (OM), carbon:nitrogen (C:N), sand, silt, clay, and sodium adsorption ratio), soil organic matter (OM) explained 68% of the variation observed for reproduction. Increasing the OM using Sphagnum sp. peat moss resulted in optimal reproduction at 7% OM (8% peat content) with the lowest variability (CV of 20%). When assessing the toxicity of a reference chemical, boric acid, the effect of peat amendment reduced lethality to adults with no observable difference on reproduction. The use an age-synchronized culture reduced the test variability for reproduction relative to the use of unsynchronized cultures. Oppia nitens is a good candidate species for a standardized test design, with adult survival easily assessed in a relatively simple design. A long-term reproduction test with O. nitens will require the use of a synchronized population and, on occasion, OM amendment when testing soils with low organic matter content.

Comparative toxicity of imidacloprid and thiacloprid to different species of soil invertebrates

“…Oppia nitens was tested following Princz et al (2010), with an exposure of 35 days. Animals were taken from the cultures at the Department of Ecological Science, Vrije Universiteit, Amsterdam.…”

Comparative ecotoxicity of chlorantraniliprole to non-target soil invertebrates

“…The toxicity test with O. nitens followed the method of Princz et al (2010). Twenty visually healthy mites with same colour shade were collected from the culture and randomly placed into test containers (5 cm diameter) with a gauze bottom, containing 25 g moist soil.…”

Soil ecotoxicology: state of the art and future directions

“…Also the toxicity tests with the oribatid mites Oppia nitens and Platynothrus peltifer described in the literature (Van Gestel and Doornekamp 1998, Princz et al 2010) are not yet standardized. These tests focus on survival and reproduction.…”