Orientation and size-dependent mechanical modulation within individual secondary osteons in cortical bone tissue

Carnelli, Davide; Vena, Pasquale; Dao, Ming; Ortiz, Christine; Contro, R.

doi:10.1098/rsif.2012.0953

Cited by 41 publications

(27 citation statements)

References 46 publications

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“…Collagen fibres' orientation within the osteon is believed to be the principal reason for differences in the micromechanical properties in cortical bone along different directions in large animals and humans [20,39–41]. Rodent bone does not present an osteonal structural organization, but their collagen fibres are also mainly oriented in an axial direction in their long bones [42].…”

Section: Discussionmentioning

confidence: 99%

Nanoindentation analysis of the micromechanical anisotropy in mouse cortical bone

et al. 2017

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Studies investigating micromechanical properties in mouse cortical bone often solely focus on the mechanical behaviour along the long axis of the bone. Therefore, data on the anisotropy of mouse cortical bone is scarce. The aim of this study is the first-time evaluation of the anisotropy ratio between the longitudinal and transverse directions of reduced modulus and hardness in mouse femurs by using the nanoindentation technique. For this purpose, nine 22-week-old mice (C57BL/6) were sacrificed and all femurs extracted. A total of 648 indentations were performed with a Berkovich tip in the proximal (P), central (C) and distal (D) regions of the femoral shaft in the longitudinal and transverse directions. Higher values for reduced modulus are obtained for indentations in the longitudinal direction, with anisotropy ratios of 1.72 ± 0.40 (P), 1.75 ± 0.69 (C) and 1.34 ± 0.30 (D). Hardness is also higher in the longitudinal direction, with anisotropic ratios of 1.35 ± 0.27 (P), 1.35 ± 0.47 (C) and 1.17 ± 0.19 (D). We observed a significant anisotropy in the micromechanical properties of the mouse femur, but the correlation for reduced modulus and hardness between the two directions is low (r2 < 0.3) and not significant. Therefore, we highly recommend performing independent indentation testing in both the longitudinal and transverse directions when knowledge of the tissue mechanical behaviour along multiple directions is required.

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Section: Discussionmentioning

confidence: 99%

Nanoindentation analysis of the micromechanical anisotropy in mouse cortical bone

et al. 2017

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“…This assembly is recurrently found in the endoskeletons and exoskeletons of mammals and invertebrates 16,17 and is closely related to outstanding mechanical performance with unprecedented impact resistance. [18][19][20] The fabrication of such exquisite variation in local texture has been recently achieved in composite materials up to 50 vol% mineral content using additive manufacturing methods based on three-dimensional (3D) printing [21][22][23][24] or magnetic alignment with slip casting (MASC). 25 These latest efforts to bring complex heterogeneous texture into polymer/ceramic composites have not yet reached dense ceramics.…”

Section: Introductionmentioning

confidence: 99%

Processing of dense bioinspired ceramics with deliberate microstructure

Ferrand

Bouville

2019

J Am Ceram Soc

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The architectures of biological hard materials reveal finely tailored complex assemblies of mineral crystals. Numerous recent studies associate the design of these local assemblies with impressive macroscopic response. Reproducing such exquisite control in technical ceramics conflicts with commonly used processing methods. Here, we circumvent this issue by combining the recently developed Magnetically Assisted Slip Casting (MASC) technique with the well‐established process of templated grain growth (TGG). MASC enables the local control over the orientation of platelets dispersed among smaller isotropic particles. After a high‐temperature pressureless heat treatment, the grains of the final ceramic follow the same orientation as of the initial platelets. This combination allows us to produce 95% dense alumina part with a grain orientation following any deliberate orientation. We successfully fabricated microstructures inspired from biological materials with ceramics that present periodically varying patterns with a programmable pitch down to a few tens of micrometers. The periodically textured dense ceramics exhibit matching variation of local hardness, confirming the capacity of the process to tailor local properties. This unique micrometer scale control over the local mechanical properties could be applied to adapt ceramic structures to complex loads using this inexpensive and scalable process. In systems where functional properties also depend on anisotropic grain orientation, the principle presented here could enable the creation of new multifunctional ceramics.

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“…Microchannel pattern decorated films were seeded with human osteoblasts (HOBs) and human adipose-derived stem cells (ADSCs) to align the cells and expected them to secrete ECM in a parallel fashion as in cortical bone. It was hypothesized that alignment could lead to anisotropic mechanical strength to resist the compressive, tensional and torsional forces [23] . In this study the organization of bone lamellae was mimicked with a collagen-fibroin-ELR blend film carrying oriented collagen fibers and the influence of cell type on the mechanical properties of the resultant scaffolds were studied using HOBs and ADSCs ( Fig.…”

Section: Introductionmentioning

confidence: 99%

Human adipose derived stem cells are superior to human osteoblasts (HOB) in bone tissue engineering on a collagen-fibroin-ELR blend

Sayın¹,

Rashid

Rodríguez‐Cabello

et al. 2017

Bioactive Materials

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The ultrastructure of the bone provides a unique mechanical strength against compressive, torsional and tensional stresses. An elastin-like recombinamer (ELR) with a nucleation sequence for hydroxyapatite was incorporated into films prepared from a collagen – silk fibroin blend carrying microchannel patterns to stimulate anisotropic osteogenesis. SEM and fluorescence microscopy showed the alignment of adipose-derived stem cells (ADSCs) and the human osteoblasts (HOBs) on the ridges and in the grooves of microchannel patterned collagen-fibroin-ELR blend films. The Young's modulus and the ultimate tensile strength (UTS) of untreated films were 0.58 ± 0.13 MPa and 0.18 ± 0.05 MPa, respectively. After 28 days of cell culture, ADSC seeded film had a Young's modulus of 1.21 ± 0.42 MPa and UTS of 0.32 ± 0.15 MPa which were about 3 fold higher than HOB seeded films. The difference in Young's modulus was statistically significant (p: 0.02). ADSCs attached, proliferated and mineralized better than the HOBs. In the light of these results, ADSCs served as a better cell source than HOBs for bone tissue engineering of collagen-fibroin-ELR based constructs used in this study. We have thus shown the enhancement in the tensile mechanical properties of the bone tissue engineered scaffolds by using ADSCs.

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Orientation and size-dependent mechanical modulation within individual secondary osteons in cortical bone tissue

Cited by 41 publications

References 46 publications

Nanoindentation analysis of the micromechanical anisotropy in mouse cortical bone

Nanoindentation analysis of the micromechanical anisotropy in mouse cortical bone

Processing of dense bioinspired ceramics with deliberate microstructure

Human adipose derived stem cells are superior to human osteoblasts (HOB) in bone tissue engineering on a collagen-fibroin-ELR blend

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