Orientation of Schizosaccharomyces POMBE Nonliving Cells under Alternating Uniform and Nonuniform Electric Fields

Iglesias, Francisco Javier Merchán; Lopez, MaCarmen; Santamaría, Carlos; Domínguez, Ángel

doi:10.1016/s0006-3495(85)83830-3

Cited by 40 publications

(14 citation statements)

References 12 publications

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“…The cell behaviour was found to depend on the medium conductivity conditions and field frequency (Iglesias et al 1985). To mimic a cell environment which is of conductivity much higher than that of pure water, we varied the salt concentration inside and outside the vesicle (Aranda et al 2006).…”

Section: Vesicles In Alternating Electric (Ac) Fieldsmentioning

confidence: 99%

A practical guide to giant vesicles. Probing the membrane nanoregime via optical microscopy

Dimova

Aranda

Bezlyepkina

et al. 2006

J. Phys.: Condens. Matter

300

321

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Research on giant vesicles is becoming increasingly popular. Giant vesicles provide model biomembrane systems for systematic measurements of mechanical and rheological properties of bilayers as a function of membrane composition and temperature, as well as hydrodynamic interactions. Membrane response to external factors (for example electric fields, ions and amphiphilic molecules) can be directly visualized under the microscope. In this paper we review our current understanding of lipid bilayers as obtained from studies on giant unilamellar vesicles. Because research on giant vesicles increasingly attracts the interest of scientists from various backgrounds, we also try to provide a concise introduction for newcomers in the field. Finally, we summarize some recent developments on curvature effects induced by polymers, domain formation in membranes and shape transitions induced by electric fields.

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Section: Vesicles In Alternating Electric (Ac) Fieldsmentioning

confidence: 99%

A practical guide to giant vesicles. Probing the membrane nanoregime via optical microscopy

Dimova

Aranda

Bezlyepkina

et al. 2006

J. Phys.: Condens. Matter

300

321

View full text Add to dashboard Cite

show abstract

“…Depending upon the properties of the particle and medium, there may be one or two "turnover" frequencies where the orientation changes from parallel to perpendicular to the field [66,67]. Not only the properties of the particle but also those of the medium are important, and these appear in the form of their time constants ε/σ [1].…”

Section: Orientationmentioning

confidence: 99%

Particle patterning using fluidics and electric fields

Arnold

2008

IEEE Trans. Dielect. Electr. Insul.

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Electric-field manipulation of micro-particles in suspension can create patterns via a number of particle forces and fluid flows. These effects are assessed for their suitability for down-scaling to form nano-patterns such as may be incorporated into structured nano-composites. Consideration is given not only to the assembly of field-aligned chains or wires, but also to methods that can give cross-field assembly and even 2-D patterns or crystals. Dielectrophoresis is often the dominant driving force behind particle assembly, but other dipole-dipole interactions and also electrically-driven fluid flows are increasingly recognized as significant or dominant. Orientation of nonspherical particles in frequency-selectable directions is also possible. Estimates for the threshold field strengths required for using these effects to handle nano-particles are considered. Finally the use of media with modified permittivity to increase the fieldinduced forces or to optimize the selectivity of particle incorporation is discussed. Index Terms -Composite; Artificial tissue; Dielectrophoresis; Electroosmosis W. Michael Arnold (A'95-SM'00) was raised in Yorkshire, U.K. He received the B.A. degree from Cambridge University in 1974, and the M.Sc. and Ph.D. (EE) degrees from the University of Wales.After developing the technique of electro-rotation and also teaching in Biotechnology at the University of Würzburg, Germany, he received the Dr. rer. nat. habil. degree from that University in 1993. In 1995 he chaired the IAS session on "Biological Applications of Electrostatics", and in 2001 he cochaired a DEIS workshop on "Fast Field Effects in Biosystems". Present research interests are nanoassembly, microfluidic devices and bio-impedance spectroscopy.

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“…Biological cells (such as erythrocytes, yeast cells) are found to orient themselves parallel or perpendicular to the direction of an external electric field [1][2][3], depending on membrane elasticity, coupling between the membrane and the cytoskeleton, excess membrane area, and also solution conductivity [4][5][6]. Due to the complexity of biological cells, the electrodeformation and electrodynamics of vesicles (closed lipid bilayer membranes) have been intensively pursued as a paradigm for understanding how a biological cell behaves under an electric field.…”

Section: Introductionmentioning

confidence: 99%

Equilibrium electrodeformation of a spheroidal vesicle in an ac electric field

Nganguia

Young

2013

Phys. Rev. E

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In this work, we develop a theoretical model to explain the equilibrium spheroidal deformation of a giant unilamellar vesicle (GUV) under an alternating (ac) electric field. Suspended in a leaky dielectric fluid, the vesicle membrane is modeled as a thin capacitive spheroidal shell. The equilibrium vesicle shape results from the balance between mechanical forces from the viscous fluid, the restoring elastic membrane forces, and the externally imposed electric forces. Our spheroidal model predicts a deformation-dependent transmembrane potential, and is able to capture large deformation of a vesicle under an electric field. A detailed comparison against both experiments and small-deformation (quasispherical) theory showed that the spheroidal model gives better agreement with experiments in terms of the dependence on fluid conductivity ratio, permittivity ratio, vesicle size, electric field strength, and frequency. The spheroidal model also allows for an asymptotic analysis on the crossover frequency where the equilibrium vesicle shape crosses over between prolate and oblate shapes. Comparisons show that the spheroidal model gives better agreement with experimental observations.

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Orientation of Schizosaccharomyces POMBE Nonliving Cells under Alternating Uniform and Nonuniform Electric Fields

Cited by 40 publications

References 12 publications

A practical guide to giant vesicles. Probing the membrane nanoregime via optical microscopy

A practical guide to giant vesicles. Probing the membrane nanoregime via optical microscopy

Particle patterning using fluidics and electric fields

Equilibrium electrodeformation of a spheroidal vesicle in an ac electric field

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