Oriented collagen nanocoatings for tissue engineering

Pastorino, Laura; Dellacasa, Elena; Scaglione, Silvia; Giulianelli, M.; Sbrana, Francesca; Vassalli, Massimo; Ruggiero, Carmelina

doi:10.1016/j.colsurfb.2013.10.026

Cited by 45 publications

(42 citation statements)

References 40 publications

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“…This natural scaffolds are frequently used for their high availability, biological plasticity, biocompatibility, biodegradability and nontoxicity [83,84] . It has been found that osteoblasts obtained from AFMSCs were able to adhere and grow well on SLA (Sandblasted and Acid Etching) titanium surfaces, materials commonly utilized in dental implantology, as revealed by electron microscopy observation [60] .…”

Section: Tissue Engineering Approaches For In Vivo Bone Regenerationmentioning

confidence: 99%

Osteogenic differentiation of amniotic fluid mesenchymal stromal cells and their bone regeneration potential

Pipino

Pandolfi

2015

WJSC

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Section: Tissue Engineering Approaches For In Vivo Bone Regenerationmentioning

confidence: 99%

Osteogenic differentiation of amniotic fluid mesenchymal stromal cells and their bone regeneration potential

Pipino

Pandolfi

2015

WJSC

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“…Recently, our team has also showed the potential of collagen films prepared by LS deposition onto commercial polymer substrates for RPE cell culture [14]. Even so, the use of LS films as biomaterials for tissue engineering applications is still in its infancy, and, to our knowledge, no records can be found on LS film deposition onto biodegradable polymer surfaces, as inorganic substrates such as mica, silicon and glass are the most commonly elected materials [25,28,29].…”

Section: Introductionmentioning

confidence: 99%

“…The principle is that collagen at low pH occurs in its molecular form, but forms fibres when applied to the interface between air and phosphate buffered saline solution, since the charge neutralisation triggers the association between molecules. When this interface is compressed at a Langmuir trough, collagen fibres become oriented and can subsequently be transferred onto a solid substrate by vertical motion of the substrate into the buffer solution and through the monolayer (Figure 1 B) [23,25,26]. Alternatively, highly oriented collagen substrates prepared by horizontal immersion of solid substrates, or LangmuirSchafer (LS) deposition (LS) [27], have been prepared with positive effects over the adhesion and proliferation of 3T3 fibroblasts [25].…”

Section: Introductionmentioning

confidence: 99%

“…When this interface is compressed at a Langmuir trough, collagen fibres become oriented and can subsequently be transferred onto a solid substrate by vertical motion of the substrate into the buffer solution and through the monolayer (Figure 1 B) [23,25,26]. Alternatively, highly oriented collagen substrates prepared by horizontal immersion of solid substrates, or LangmuirSchafer (LS) deposition (LS) [27], have been prepared with positive effects over the adhesion and proliferation of 3T3 fibroblasts [25]. Recently, our team has also showed the potential of collagen films prepared by LS deposition onto commercial polymer substrates for RPE cell culture [14].…”

Section: Introductionmentioning

confidence: 99%

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Langmuir-Schaefer film deposition onto honeycomb porous films for retinal tissue engineering

et al. 2017

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Age-related macular degeneration (AMD) is the leading cause of vision loss in senior citizens in the developed world. The disease is characterised by the degeneration of a specific cell layer at the back of the eye -the retinal pigment epithelium (RPE), which is essential in retinal function. The most promising therapeutic option to restore the lost vision is considered to be RPE cell transplantation. This work focuses on the development of biodegradable biomaterials with similar properties to the native Bruch's membrane as carriers for RPE cells. In particular, the breath figure (BF) method was used to create semi-permeable microporous films, which were thereafter used as the substrate for the consecutive Langmuir-Schaefer (LS) deposition of highly organised layers of collagen type I and collagen type IV. The newly developed biomaterials were further characterised in terms of surface porosity, roughness, hydrophilicity, collagen distribution, diffusion properties and hydrolytic stability.Human embryonic stem cell-derived RPE cells (hESC-RPE) cultured on the biomaterials showed good adhesion, spreading and morphology, as well as the expression of specific protein markers. Cell function was additionally confirmed by the assessment of the phagocytic capacity of hESC-RPE.Throughout the study, microporous films consistently showed better results as cell culture materials for 2 hESC-RPE than dip-coated controls. This work demonstrates the potential of the BF-LS combined technologies to create biomimetic prosthetic Bruch's membranes for hESC-RPE transplantation.

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“…Several organic matrices, such as self-assembled monolayers (Tanahashi and Matsuda 1997;Han and Aizenberg 2003), polymers (Wei and Ma 2004;Xu et al 2014;Nogueira et al 2016), and collagen-based structures (Ehrlich 2010;Pastorino et al 2014;Jin et al 2015), have been used to study the growth of biominerals. However, there has been little exploration of the use of organic matrices to mediate the formation of hybrid coatings on metallic surfaces (Walsh et al 2001;Xia et al 2012).…”

Section: Bio-inspired Modifications Of Surfacesmentioning

confidence: 99%

Biomedical applications of nanotechnology

et al. 2017

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The ability to investigate substances at the molecular level has boosted the search for materials with outstanding properties for use in medicine. The application of these novel materials has generated the new research field of nanobiotechnology, which plays a central role in disease diagnosis, drug design and delivery, and implants. In this review, we provide an overview of the use of metallic and metal oxide nanoparticles, carbon-nanotubes, liposomes, and nanopatterned flat surfaces for specific biomedical applications. The chemical and physical properties of the surface of these materials allow their use in diagnosis, biosensing and bioimaging devices, drug delivery systems, and bone substitute implants. The toxicology of these particles is also discussed in the light of a new field referred to as nanotoxicology that studies the surface effects emerging from nanostructured materials.

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Oriented collagen nanocoatings for tissue engineering

Cited by 45 publications

References 40 publications

Osteogenic differentiation of amniotic fluid mesenchymal stromal cells and their bone regeneration potential

Osteogenic differentiation of amniotic fluid mesenchymal stromal cells and their bone regeneration potential

Langmuir-Schaefer film deposition onto honeycomb porous films for retinal tissue engineering

Biomedical applications of nanotechnology

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