Oriented conjugation of antibodies against the epithelial cell adhesion molecule on fluorescently doped silica nanoparticles for flow-cytometric determination and in vivo imaging of EpCAM, a biomarker for colorectal cancer

Treerattrakoon, Kiatnida; Chanthima, Warangkana; Apiwat, Chayachon; Dharakul, Tararaj

doi:10.1007/s00604-017-2211-6

Cited by 16 publications

(15 citation statements)

References 26 publications

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“…Currently, ensemble methods like Western blot, BCA assay and ζ-potential are used as indicators for changes in surface physical features and biomolecule functionalization of materials. [75][76][77][78][79] By using STORM or DNA-PAINT, these characteristics can be resolved on a single molecule level. Later, counting of integer numbers of molecules can be achieved.…”

Section: Informative and Quantitative Outputmentioning

confidence: 99%

Can super-resolution microscopy become a standard characterization technique for materials chemistry?

et al. 2022

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Section: Informative and Quantitative Outputmentioning

confidence: 99%

Can super-resolution microscopy become a standard characterization technique for materials chemistry?

et al. 2022

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“…However, the potential of using such DDSNs for labelling, identifying and quantifying CTCs in patient samples is very challenging as there are only few CTCs per billion of blood cells (Shen et al 2017). The use of anti-EpCAM as a targeting biomolecule was also shown by Treerattrakoon et al (2017) when Cy5-doped silica NPs coated with the monoclonal antibody was used to localise the colloids in HT-29 colorectal tumours.…”

Section: In Vitromentioning

confidence: 99%

Dye-doped silica nanoparticles: synthesis, surface chemistry and bioapplications

et al. 2020

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Background: Fluorescent silica nanoparticles have been extensively utilised in a broad range of biological applications and are facilitated by their predictable, wellunderstood, flexible chemistry and apparent biocompatibility. The ability to couple various siloxane precursors with fluorescent dyes and to be subsequently incorporated into silica nanoparticles has made it possible to engineer these fluorophores-doped nanomaterials to specific optical requirements in biological experimentation. Consequently, this class of nanomaterial has been used in applications across immunodiagnostics, drug delivery and human-trial bioimaging in cancer research. Main body: This review summarises the state-of-the-art of the use of dye-doped silica nanoparticles in bioapplications and firstly accounts for the common nanoparticle synthesis methods, surface modification approaches and different bioconjugation strategies employed to generate biomolecule-coated nanoparticles. The use of dye-doped silica nanoparticles in immunoassays/biosensing, bioimaging and drug delivery is then provided and possible future directions in the field are highlighted. Other non-cancerrelated applications involving silica nanoparticles are also briefly discussed. Importantly, the impact of how the protein corona has changed our understanding of NP interactions with biological systems is described, as well as demonstrations of its capacity to be favourably manipulated. Conclusions: Dye-doped silica nanoparticles have found success in the immunodiagnostics domain and have also shown promise as bioimaging agents in human clinical trials. Their use in cancer delivery has been restricted to murine models, as has been the case for the vast majority of nanomaterials intended for cancer therapy. This is hampered by the need for more human-like disease models and the lack of standardisation towards assessing nanoparticle toxicity. However, developments in the manipulation of the protein corona have improved the understanding of fundamental bio-nano interactions, and will undoubtedly assist in the translation of silica nanoparticles for disease treatment to the clinic.

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“…Using traditional analytical methods, it would be very difficult to determine this effect in vivo. However, with recent advances using in vivo imaging and mass spec techniques, it could be possible to observe the effect of blood fluid dynamics and the interaction of CMAs with endothelial walls of the vasculature as well as nanoparticle penetration through the vasculature [81][82][83][84].…”

Section: Tumor Vascularity Perfusion and Permeabilitymentioning

confidence: 99%

Complex Effects of Tumor Microenvironment on the Tumor Disposition of carrier-mediated Agents

Lucas

Price

Schorzman

et al. 2017

Nanomedicine (Lond.)

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Major advances in carrier-mediated agents, including nanoparticle, conjugates and antibody-drug conjugates, have created revolutionary drug delivery systems in cancer over the past two decades. While these agents provide several advantages, such as greater duration of exposure and solubility, compared with their small-molecule counterparts, there is substantial variability in delivery of these agents to tissues and especially tumors. This review provides an overview of tumor microenvironment factors that affect the pharmacokinetics and pharmacodynamics of carrier-mediated agents observed in preclinical models and patients.

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Oriented conjugation of antibodies against the epithelial cell adhesion molecule on fluorescently doped silica nanoparticles for flow-cytometric determination and in vivo imaging of EpCAM, a biomarker for colorectal cancer

Cited by 16 publications

References 26 publications

Can super-resolution microscopy become a standard characterization technique for materials chemistry?

Can super-resolution microscopy become a standard characterization technique for materials chemistry?

Dye-doped silica nanoparticles: synthesis, surface chemistry and bioapplications

Complex Effects of Tumor Microenvironment on the Tumor Disposition of carrier-mediated Agents

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