Origin and genetic diversity of canine parvovirus 2c circulating in Mexico

Faz, Mirna; Martínez, José Simón; Gómez, Lina Marcela González; Hernández, Israel; Fajardo, Raúl; Del-Angel-Caraza, Javier

doi:10.1007/s00705-018-4072-7

Cited by 12 publications

(6 citation statements)

References 41 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…To confirm CPV-2-positive samples, PCR amplification of VP2 was conducted using the Forward Ext1F primer (5′-ATGAGTGATGGAGCAGTTCA-3′) and the Ext3R Reverse primer (5′-AGGTGCTAGTTGAGATTTTTCATATAC-3′) described by Ref. 26 . In all cases, reactions were performed in a total volume of 25 ul containing 1 unit of Taq polymerase (Go taq flexi-Promega), 1 × Taq buffer, 2 µM MgCl 2 , 0.5 µM dNTPs, 20 pmol of each primer and 100 ng of extracted DNA.…”

Section: Methodsmentioning

confidence: 99%

First detection and full genomic analysis of Canine Circovirus in CPV-2 infected dogs in Colombia, South America

Giraldo-Ramirez

Rendón-Marín

Vargas-Bermúdez

et al. 2020

Sci Rep

View full text Add to dashboard Cite

Canine Circovirus (CanineCV) is an emerging virus which since its first report in USA in 2012, it has been described worldwide. It was the second mammalian circovirus species identified in dogs and its role in canine enteritis is still being uncertain as much as its association in disease with the Canine Parvovirus-2 (CPV-2). Here, we aim to confirm for the first time the presence of CanineCV in Colombia and to develop phylogenetic evolutive analyses of CanineCV in CPV-2 positive animals. DNA from samples were extracted and PCR, full genome sequencing and phylogenetic analysis was performed to detect and characterize CanineCV. From a total of 30 CPV-2 positive samples, 16.6% (n = 5) were positives for CanineCV. Sequencing analysis of Colombian CanineCV wild-type strains displayed high identity to each other (99.5–99.7% nt; 99.7% aa). The full genome phylogenetic analysis confirmed that worldwide reported CanineCV strains were separated into four distinct genotypes in addition to a European origin of the South American CanineCV strains. This study demonstrated the importance of continue surveillance of emerging viruses in canine populations and confirm for the first time the circulation and origin of CanineCV in Colombia.

show abstract

Section: Methodsmentioning

confidence: 99%

First detection and full genomic analysis of Canine Circovirus in CPV-2 infected dogs in Colombia, South America

Giraldo-Ramirez

Rendón-Marín

Vargas-Bermúdez

et al. 2020

Sci Rep

View full text Add to dashboard Cite

show abstract

“…In South America, new CPV-2a, or other mutants of CPV-2a (VP2 Ser297Asn, Phe267Tyr, Tyr324Ile, and Thr440Ala) have been found to coexist with CPV-2b and/or CPV-2c variants at various frequencies, emerging as the major strain in a few studies [13,21,[28][29][30][31][32][33]. In studies from North (Canada and USA) and Central (Mexico) Americas, CPV-2b, or CPV-2c was most prevalent, while a single report from Alaska detected both new CPV-2a and new CPV-2b in an outbreak of canine gastroenteritis [13,[34][35][36][37]. Taken together, the molecular epidemiology of CPV-2 in the Caribbean region appears to differ from those reported in nearby North, Central, and South American countries, although further studies on the other islands are required to validate this observation.…”

Section: Molecular Investigation Of Cpv-2 Outbreak In Nevis Islandmentioning

confidence: 99%

Molecular Investigation of Canine Parvovirus-2 (CPV-2) Outbreak in Nevis Island: Analysis of the Nearly Complete Genomes of CPV-2 Strains from the Caribbean Region

Gainor

Bowen

Bolfă

et al. 2021

Viruses

View full text Add to dashboard Cite

To date, there is a dearth of information on canine parvovirus-2 (CPV-2) from the Caribbean region. During August–October 2020, the veterinary clinic on the Caribbean island of Nevis reported 64 household dogs with CPV-2-like clinical signs (hemorrhagic/non-hemorrhagic diarrhea and vomiting), of which 27 animals died. Rectal swabs/fecal samples were obtained from 43 dogs. A total of 39 of the 43 dogs tested positive for CPV-2 antigen and/or DNA, while 4 samples, negative for CPV-2 antigen, were not available for PCR. Among the 21 untested dogs, 15 had CPV-2 positive littermates. Analysis of the complete VP2 sequences of 32 strains identified new CPV-2a (CPV-2a with Ser297Ala in VP2) as the predominant CPV-2 on Nevis Island. Two nonsynonymous mutations, one rare (Asp373Asn) and the other uncommon (Ala262Thr), were observed in a few VP2 sequences. It was intriguing that new CPV-2a was associated with an outbreak of gastroenteritis on Nevis while found at low frequencies in sporadic cases of diarrhea on the neighboring island of St. Kitts. The nearly complete CPV-2 genomes (4 CPV-2 strains from St. Kitts and Nevis (SKN)) were reported for the first time from the Caribbean region. Eleven substitutions were found among the SKN genomes, which included nine synonymous substitutions, five of which have been rarely reported, and the two nonsynonymous substitutions. Phylogenetically, the SKN CPV-2 sequences formed a distinct cluster, with CPV-2b/USA/1998 strains constituting the nearest cluster. Our findings suggested that new CPV-2a is endemic in the region, with the potential to cause severe outbreaks, warranting further studies across the Caribbean Islands. Analysis of the SKN CPV-2 genomes corroborated the hypothesis that recurrent parallel evolution and reversion might play important roles in the evolution of CPV-2.

show abstract

“…Small intestinal intussusception can occur and create a painful, firm, tubular soft tissue mass effect found on abdominal palpation. 38 , 39 The presence and severity of clinical signs can be predictive of the length of time required in hospital. 40 …”

Section: Clinical Signs and Additional Diagnostic Testingmentioning

confidence: 99%

Update on Canine Parvoviral Enteritis

Mazzaferro

2020

Veterinary Clinics of North America: Small Animal Practice

View full text Add to dashboard Cite

Canine parvoviral enteritis is one of the most common causes of morbidity and mortality in dogs worldwide. Tests can detect viral antigen in feces, and characteristic decreases in total leukocyte, neutrophil, and lymphocyte counts can increase the index of suspicion in affected cases and can be used to prognosticate morbidity and mortality. The standard of care for infected animals includes IV crystalloid and sometimes colloid fluids, antiemetics, broad-spectrum antibiotics, and early enteral nutrition. Vaccination induces protective immunity in most dogs. Vaccination, along with limiting exposure in young puppies, is the most effective means of preventing parvoviral enteritis in dogs.

show abstract

Origin and genetic diversity of canine parvovirus 2c circulating in Mexico

Cited by 12 publications

References 41 publications

First detection and full genomic analysis of Canine Circovirus in CPV-2 infected dogs in Colombia, South America

First detection and full genomic analysis of Canine Circovirus in CPV-2 infected dogs in Colombia, South America

Molecular Investigation of Canine Parvovirus-2 (CPV-2) Outbreak in Nevis Island: Analysis of the Nearly Complete Genomes of CPV-2 Strains from the Caribbean Region

Update on Canine Parvoviral Enteritis

Contact Info

Product

Resources

About