Origin of samples of Cannabis sativa through insect fragments associated with compacted hemp drug in South America

Macedo, Marcos Patrício; Kosmann, Cecília; Pujol-Luz, José Roberto

doi:10.1590/s0085-56262013005000008

Cited by 9 publications

(6 citation statements)

References 25 publications

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“…This could be used for regulatory purposes, e.g. to trace the origin of illegal plant material confiscated by customs [30]. Besides conservation-driven biomonitoring, dried plant material is well suited for pest management.…”

Section: Discussionmentioning

confidence: 99%

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The bug in a teacup—monitoring arthropod–plant associations with environmental DNA from dried plant material

et al. 2022

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Environmental DNA analysis (eDNA) has revolutionized the field of biomonitoring in the past years. Various sources have been shown to contain eDNA of diverse organisms, for example, water, soil, gut content and plant surfaces. Here we show that dried plant material is a highly promising source for arthropod community eDNA. We designed a metabarcoding assay to enrich diverse arthropod communities while preventing amplification of plant DNA. Using this assay, we analysed various commercially produced teas and herbs. These samples recovered ecologically and taxonomically diverse arthropod communities, a total of over a thousand species in more than 20 orders, many of them specific to their host plant and its geographical origin. Atypically for eDNA, arthropod DNA in dried plants shows very high temporal stability, opening up plant archives as a source for historical arthropod eDNA. Considering these results, dried plant material appears excellently suited as a novel tool to monitor arthropods and arthropod–plant interactions, detect agricultural pests and identify the geographical origin of imported plant material. The simplicity of our approach and the ability to detect highly diverse arthropod communities from all over the world in tea bags also highlights its utility for outreach purposes and to raise awareness about biodiversity.

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Section: Discussionmentioning

confidence: 99%

“…This could be used for regulatory purposes, e.g. to trace the origin of illegal plant material confiscated by customs [30].…”

Section: Discussionmentioning

confidence: 99%

The bug in a teacup—monitoring arthropod–plant associations with environmental DNA from dried plant material

et al. 2022

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“…Forensic entomology is most widely recognized for evaluating the minimum time since death (minimum PMI) via insect colonization. However, it can also be applied to a wide range of cases including abuse and neglect ( Benecke et al 2004 ), poaching ( Anderson 1999 ), trafficking of illegal substances ( Crosby et al 1986 , Macedo et al 2013 ), and establishing a suspect’s location ( Parker 2007 ). This information provides crucial physical evidence which must be handled according to appropriate protocols, similar to DNA or fingerprints.…”

Section: Team 1 Stance: Yes Forensic Entomology Is Viable In Criminal...mentioning

confidence: 99%

Entomology beyond research and education: 2022 student debates

Sandhi,

Pickens,

Bello

et al. 2023

Journal of Insect Science

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The 2022 student debates of the Entomological Society of America (ESA) happened during the Joint Annual Meeting of the Entomological Societies of America, Canada, and British Columbia in Vancouver, BC, and addressed entomological aspects beyond research and education. The Student Debates Subcommittee of the ESA Student Affairs Committee and the participating student team members communicated for 8 months and prepared for the debates. The theme of the ESA meeting in 2022 was “Entomology as inspiration: Insects through art, science, and culture”. There were 2 unbiased speakers who introduced the debate topics as well as 4 teams who debated the following 2 topics: (i) Is forensic entomology viable in criminal case investigations and court cases today? and (ii) Are insects being treated ethically in scientific research? The teams prepared for about 8 months, debated their arguments, and shared their thoughts with the audience. The teams were judged by a panel and the winners were recognized at the ESA Student Awards Session during the annual meeting.

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“…Isso faz com que esse grupo de moscas seja importante evidência em investigações de homicídios [4,5]. Ainda, além da estimativa do IPMmin, insetos já foram tomados como indícios de movimentação de cadáveres e de localização de regiões produtoras de drogas [6,7].…”

Section: Introductionunclassified

Morfometria geométrica alar como ferramenta para a identificação de Lucilia sericata e Calliphora vicina (Diptera: Calliphoridae)

Macedo¹

2016

Rev. Bras. Crimin.

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Lucilia sericata (Meigen) e Calliphora vicina (Robineau-Desvoidy) (Diptera, Calliphoridae) estão entre as espécies de dípteros de interesse forense. A correta identificação do espécime, ou fragmento deste, coletado em um local de crime é etapa fundamental para o emprego da entomologia forense em investigações criminais. Nesse estudo, avaliou-se a possibilidade de discriminação entre duas espécies de califorídeos pela morfologia alar, por meio de análises de morfometria geométrica. Foram analisadas as asas esquerdas de 253 espécimes, sendo 119 indivíduos de C. vicina e 134 de L. sericata, por meio de análises de variáveis canônicas e análises discriminantes. Das 253 comparações par a par, 2 erros de identificação (0,7%) foram registrados para análise discriminante, enquanto 3 erros de classificação (2,3%) foram registrados para o teste de validação cruzada. Esse resultado sugere um alto índice de confiabilidade da técnica. Mais estudos são necessários para a validação dessa técnica para seu uso na prática forense.

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Origin of samples of Cannabis sativa through insect fragments associated with compacted hemp drug in South America

Cited by 9 publications

References 25 publications

The bug in a teacup—monitoring arthropod–plant associations with environmental DNA from dried plant material

The bug in a teacup—monitoring arthropod–plant associations with environmental DNA from dried plant material

Entomology beyond research and education: 2022 student debates

Morfometria geométrica alar como ferramenta para a identificação de Lucilia sericata e Calliphora vicina (Diptera: Calliphoridae)

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