Original Contribution: Wound‐healing properties of nut oil from Pouteria lucuma

Abstract

Summary Background Cell migration, angiogenesis, inflammation, and extracellular matrix remodeling are key events in wound healing. Natural products, including fatty acids (FAs), can accelerate wound healing by modulating the aforementioned events. Aims This study aims to evaluate the effect of lucuma (Pouteria lucuma O Kezte) nut oil (LNO) on fibroblasts migration, angiogenesis, inflammation, bacterial and fungal growth, and wound healing. Methods GC–MS analysis of FAs methyl esters (FAMES) was used for chemical characterization of LNO. In vitro studies were carried out with LNO investigating the induction of cell migration, cytoskeleton remodeling of human fibroblasts, inhibition of LPS-induced nitric oxide production in macrophages, and antibacterial and antifungal effects. Two in vivo studies were carried out to study LNO’s effect on angiogenesis and wound healing: (i) tail fin regeneration in transgenic zebrafish larvae expressing enhanced green fluorescent protein (EGFP) in vascular endothelial cells was used to study vessel sprouting and wound healing and (ii) the closure of wounds was evaluated in CD-1 mice after topical applications of LNO-containing formulations. Results Lucuma nut oil is a mixture of FAs, 99.7% of which were characterized. Major components of LNO (w/w) are linoleic acid (38.9%), oleic acid (27.9%), palmitic acid (18.6%), stearic acid (8.9%), and γ linolenic acid (2.9%). In vitro studies showed that LNO significantly promoted migration and vinculin expression in human fibroblasts. LNO decreased LPS-induced nitric oxide production and did not display significant antibacterial or antifungal effects. LNO induced tail fin regeneration in transgenic zebrafish larvae 48 h after tail fin amputation and significantly accelerated cutaneous wound closure in CD-1 mice. Conclusions Natural FAs from P. lucuma nut promote skin regeneration and, thus, may have applications in medicine and skin care.

Evaluation of Therapeutic Intervention with a Natural Product in Cutaneous Wound Healing: The Use of Capybara Oil

“…This process requires interactions between a variety of cell types, multiple cytokines, growth factors, and extracellular matrix (ECM) molecules [1]. Based on traditional medicine, studies have shown improvement in the healing process with the use of natural products [2], including vegetable [3–5] and animal oils [2, 6–8]. Fatty acids (FAs) from these oils can modulate events such as inflammation, cell migration, angiogenesis, and ECM remodeling [5], and they can also act as chemotactic agents for leukocytes and promote cell proliferation [9, 10].…”

IMPORTANCIA DE LOS HUERTOS FAMILIARES EN LA ESTRATEGIA DE DIVERSIFICACIÓN DEL INGRESO Y EN LA CONSERVACIÓN in situ DE Pouteria lucuma [R et. Pav] O. Kze.

“…En el caso de la lúcuma, el plan de desarrollo deberá considerar a los productores, acopiadores, minoristas, mayoristas y la industria alimentaria como actores directos y las instituciones locales, junto a las limitaciones enunciadas por Díaz (2006) e incursionar en la competitividad orientada al comercio justo y aspectos ambientales (Eyzaguirre & Dennis, 2007;Ayuya et al, 2015) en mérito a sus propiedades nutracéuticas y cosméticas (National Research Council, 1989;Gonzales, 2007;Glorio et al, 2008;Rojo et al, 2010;Dini, 2011).…”

“…Pav] O. Kze, de la familia de las Sapotaceae (Baehni y Bernardi, 1970), es una fruta nativa (León, 2000) que ha contribuido en la dieta básica de las familias pobres (National Research Council, 1989) y es alimento de varias especies de la fauna silvestre en la Amazonía (Gonzales, 2007). Por su incursión en la industria alimentaria (Gamboa et al, 2014), su sabor único altamente apreciado, sus propiedades nutracéuticas, cosméticas y medicinales (Glorio et al, 2008;Rojo et al, 2010;Dini, 2011), tiene un mercado interno y externo promisorio. La lúcuma se cultiva en huertos comerciales pero con mayor frecuencia en huertos familiares.…”

Fatty Acids Profile of Coffea arabica L. Resistant to Leaf Rust Grown in Two Environments of Minas Gerais, Brazil

“…The gamma linolenic fatty acid (GLA) is a metabolite from the linoleic fatty acid and the first intermediate in the conversion of linoleic acid into arachidonic acid (Gustone, 1992). It is widely found in plants (Guil-Guerreiro, 2000;Knothe, 2013;Tso et al, 2013;Rojo et al, 2010). Some researchers have shown through the microbiologic analysis, that the gamma linolenic acid beyond its healing and nutritional characteristics, this fatty acid also shows antimicrobial characteristic (Desbois & Lawlor, 2013;Clement et al, 2008;Giamarellos-Bourboulis et al, 2004;Ramadan et al, 2008).…”