Ornamental cabbage (Brassica oleracea var. acephala) responses to phytase enzyme purified from Lactobacillus coryniformis application

Abstract

In order to increase the quality and yield of ornamental plants, especially potted ornamental plants, it is necessary to enrich the physical properties of the growing medium and to ensure the continuity of the growing medium. In order to achieve this, organic substances that create a serious cost in ornamental plant cultivation are added to the growing medium. This study was planned to assess the role of inoculation of different levels in the seeds and soaking times of purified phytase, on the plant growth and ornamental plant decorative values in ornamental cabbage plants under nutrient limiting condition in greenhouse. Different doses (E0: 0 EU, E1: 5 EU, E2: 10 EU), soaking times (W15: 15 min, W30: 30 min, W60: 60 min), and their combinations (W15 + E0, W15 + E1, W15 + E2, W30 + E0, W30 + E1, W30 + E2, W60 + E0, W60 + E1, W60 + E2) of phytase enzyme purified and isolated from the Lactobacillus coryniformis were applied to ornamental cabbage seeds, and they were sown in plug trays filled with appropriate growing medium. Seedlings were planted in plastic pots during their period when the seedlings had four to five true leaves. Treatments of phytase enzyme purified and isolated from the microorganism generally improved the observed parameters. The application of, especially, the highest level of phytase enzyme doses increased the plant height, main stem height, and stem diameter of ornamental cabbage as compared to control (E0 treatment: distilled water). While the highest number of leaves per plant was obtained at E1 and E2 application doses and W30 and W60 soaking times; the highest stem diameter was obtained at E2 application doses and W30 and W60 soaking times. The present study clarified that the purified phytase enzyme can increase ornamental cabbage quality at the appropriate concentration and soaking time and is a promising biotechnology material for agricultural applications, and especially in different ornamental plant species.

Purification and Biochemical Characterization of Phytase Enzyme from Lactobacillus brevis Isolated from Fresh Kashar Cheese

“…Dikbaş et al [8] determined the phytase activity of L. brevis isolated from kashar cheese as 212.97 U/mL. Dikbaş et al [15] reported the specific activity of phytase enzyme purified and isolated from L. coryniformis as 188.31 (EU mg -1 protein).The data obtained in our study are consistent with the literature.…”

“…Phytase, one of the industrial enzymes, plays an important role in the field of biotechnology due to its worldwide adoption and being the subject of various studies [9]. Phytases exhibit exceptional versatility in a variety of fields, including human dietary enrichment, animal feed development and industrial processes, driving interest in the discovery of new microorganisms capable of producing phytases [15].…”

“…In this study, the concentration of the protein was measured as 0.989 mg/mL after partial purification. Dikbaş et al [8] from L. brevis and Dikbaş et al [15] from L. coryniformis determined the protein value of the phytase enzyme they purified as 0.504 mg/mL and 0.16 mg/mL, respectively. Karagöz et al [11] reported the protein concentration of phytase purified from L. plantarum as 0.11 mg/mL.…”

“…Phytases are isolated from many organisms such as plants, bacteria and fungi [10,13,14]. However, it seems that plants are more preferred than other phytase sources in commercial industrial applications due to the catalytic properties of microbial phytases, their abundance, versatility, easier production, economic stability and environmental friendliness [13,15,16]. The application of microorganisms or enzymes isolated from them in sectors vital to human health is a subject of debate concerning their safety.…”

Evaluating Enterococcus faecium9 N‐2 as a probiotic candidate from traditional village white cheese

“…Therefore, the ability of the strain to produce phytase enzyme was determined, and the strain exhibited an enzyme activity of 244 U/mL. Similar to the results obtained, there are many studies on LAB phytase production (Demir et al, 2018;Dikbaş, Parlakova Karagöz, et al, 2023;Dikbaş, Uçar, & Alım, 2023;Karagöz et al, 2021).…”