Ornithobacterium rhinotracheale has neuraminidase activity causing desialylation of chicken and turkey serum and tracheal mucus glycoproteins

Kastelic, Saša; Berčič, Rebeka Lucijana; Cizelj, Ivanka; Benčina, Mateja; Makrai, László; Zorman-Rojs, Olga; Narat, Mojca; Bisgaard, Magne; Benčina, Dušan

doi:10.1016/j.vetmic.2012.09.018

Cited by 8 publications

(7 citation statements)

References 17 publications

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“…The ORT's pathogenesis severity is associated with the environments factors, biofilm formation, and synergism with others pathogens, contributing to the persistence of the microorganism ( Figure 1) [49]. The production (expression) of a hemagglutinin and neuraminidase have been described in ORT [50]. These virulence factors may play an important role in tissue colonization and inflammation in the host [51].…”

Section: Clinical Signs and Pathological Lesionsmentioning

confidence: 99%

“…These virulence factors may play an important role in tissue colonization and inflammation in the host [51]. In addition, although ORT hemolysin may also act as a virulence factor, recent studies The production (expression) of a hemagglutinin and neuraminidase have been described in ORT [50]. These virulence factors may play an important role in tissue colonization and inflammation in the host [51].…”

Section: Clinical Signs and Pathological Lesionsmentioning

confidence: 99%

See 1 more Smart Citation

Ornithobacterium rhinotracheale: An Update Review about An Emerging Poultry Pathogen

Barbosa

Cardoso

Silva

et al. 2019

Veterinary Sciences

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Respiratory diseases in birds generate sanitary and economic impacts and may be related to the environment and climate. Mycoplasma gallisepticum, Ornithobacterium rhinotracheale (ORT), Pasteurella multocida, Avibacterium paragallinarum, Escherichia coli, Riemerella anatipestifer, and Bordetella avium are among the most important avian respiratory pathogens. ORT is responsible for causing ornitobacteriosis, a disease characterized by clinical signs ranging from mild to severe respiratory conditions, with high mortality rates, mainly affecting turkeys and chickens. The first report of ornitobacteriosis was in 1981 in Germany. Despite its importance, few studies on ORT have been published. In addition, the presence of this pathogen has been neglected in poultry farms, mainly due to the lack of appropriate diagnostic protocols. The lack of correct isolation and diagnostic protocols along with inappropriate use of antimicrobial agents have been contributing to treatment failure. Due to its economic importance to the poultry industry, ornitobacteriosis should be monitored and included in national programs for the prevention and control of avian respiratory diseases. This review aimed to update and discuss important issues related to ORT since this pathogen has great economic and sanitary implications for the chicken production chain.

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Section: Clinical Signs and Pathological Lesionsmentioning

confidence: 99%

Section: Clinical Signs and Pathological Lesionsmentioning

confidence: 99%

Ornithobacterium rhinotracheale: An Update Review about An Emerging Poultry Pathogen

Barbosa

Cardoso

Silva

et al. 2019

Veterinary Sciences

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“…Neuraminidase (NA) is an exosialidase enzyme, commonly found in many pathogenic microbes and viruses such as Vibrio cholera, 1 Streptococcus pneumonia, 2 Corynebacterium diptheriae, 3 Erysipelothrix rhusiopathiae, 4 Clostridium perfringens, 5 Ornithobacterium rhinotracheale, 6 avian influenza (AI) virus and Newcastle disease (ND) virus. 7 NA cleaves A-ketosidic linkage between sialic acid and an adjacent sugar residue, and facilitates viral release from the host cell surface to the target cell.…”

Section: Introductionmentioning

confidence: 99%

Electrochemical Behavior of Zanamivir at Gold-Modified Boron-Doped Diamond Electrodes for an Application in Neuraminidase Sensing

et al. 2015

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This study emphasizes the electrochemical detection of Neuraminidase (NA) based on NA inhibition by Zanamivir. The detection method was developed based on the difference of electrochemical responses of Zanamivir in the presence and the absence of NA in phosphate buffer solution (pH 5.5). Gold and gold-modified boron-doped diamond was used as the working electrode and the measurement was conducted using cyclic voltammetry method. A linear calibration curve of Zanamivir was observed in the concentration range of 5 × 10−4 mol/L (R 2 = 0.991), with the estimated limit of detection (LOD) of 1.49 × 10 −6 mol/L. The presence of NA increases the peak current of gold oxidation and reduction with linear calibration curves were monitored in the concentration range of 0-15 mU (R 2 = 0.996). An estimated LOD of 0.25 mU NA and an excellent reproducibility of the detection with an RSD of 1.18% can be achieved. Application of a real sample was successfully demonstrated for NA detection in the presence of mucin, suggested that the method is promising for pharmaceutical or medical application.

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“…This bacterium is able to adhere to tracheal cells in chickens and possesses a haemagglutinin and a neuraminidase (Soriano et al, 2003;Vega et al, 2008;De Haro-Cruz et al, 2013;Kastelic et al, 2013); additionally, it has been reported that some ORT isolates are able to produce a biofilm (Vichi et al, 2012). A biofilm is considered to be a complex microbial community where microorganisms synthesize an exopolysaccharide matrix that protects the microorganisms against the environment, antimicrobial agents and the immune response of the host, it can adhere to either an inert surface or a live tissue (Lasa et al, 2005;Vila et al, 2008;Castrillón et al, 2010).…”

Section: Introductionmentioning

confidence: 99%

Conditions that induce biofilm production byOrnithobacterium rhinotracheale

Rosa-Ramos¹,

Rodríguez-Cruz

López-Villegas

et al. 2015

Avian Pathology

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Ornithobacterium rhinotracheale (ORT) is a Gram-negative bacillus that causes respiratory disease in birds, and directly affects the poultry industry. The mechanisms behind these infections are not completely known. Currently, its capacity to form biofilms on inert surfaces has been reported; however, the conditions for biofilm development have not been described yet. The present work was aimed at identifying the conditions that enhance in vitro biofilm formation and development by ORT. For this, serovars A-E were analysed to assess their ability to induce biofilm development on 96-well flat-bottom polystyrene microtitre plates under diverse conditions: temperature, incubation time, and CO 2 concentration. The results obtained showed not only that all serovars have the ability to produce in vitro biofilms, but also that the optimal conditions for biofilm density were 40°C after 72 h at an elevated CO 2 concentration. In conclusion, ORT biofilm formation depends on the environmental conditions and may contribute to the persistence of this microorganism.

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Ornithobacterium rhinotracheale has neuraminidase activity causing desialylation of chicken and turkey serum and tracheal mucus glycoproteins

Cited by 8 publications

References 17 publications

Ornithobacterium rhinotracheale: An Update Review about An Emerging Poultry Pathogen

Ornithobacterium rhinotracheale: An Update Review about An Emerging Poultry Pathogen

Electrochemical Behavior of Zanamivir at Gold-Modified Boron-Doped Diamond Electrodes for an Application in Neuraminidase Sensing

Conditions that induce biofilm production byOrnithobacterium rhinotracheale

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