Orthodenticle homeobox OTX1 is a potential prognostic biomarker for bladder cancer

Jiang, Lei; Zuo, Zhongqiang; Lin, Jie; Yang, Chuanfeng

doi:10.1080/21655979.2021.1974646

Cited by 15 publications

(10 citation statements)

References 60 publications

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“…OTX1 is a transcription factor that is required for proper brain and sensory organ development in mice [ 68 ], where it regulates cell cycle progression, with its knockdown leading to diminished neurons but increased astrocytes [ 69 ]. Few studies have linked OTX1 overexpression with tumorigenesis and growth in cancers [ 70 , 71 ], and to the best of our knowledge, it has not been yet reported as an epigenetic marker in brain cancers.…”

Section: Discussionmentioning

confidence: 99%

Correlation between DNA Methylation and Cell Proliferation Identifies New Candidate Predictive Markers in Meningioma

Hergalant

Saurel

Divoux

et al. 2022

Cancers

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Meningiomas are the most common primary tumors of the central nervous system. Based on the 2021 WHO classification, they are classified into three grades reflecting recurrence risk and aggressiveness. However, the WHO’s histopathological criteria defining these grades are somewhat subjective. Together with reliable immunohistochemical proliferation indices, other molecular markers such as those studied with genome-wide epigenetics promise to revamp the current prognostic classification. In this study, 48 meningiomas of various grades were randomly included and explored for DNA methylation with the Infinium MethylationEPIC microarray over 850k CpG sites. We conducted differential and correlative analyses on grade and several proliferation indices and markers, such as mitotic index and Ki-67 or MCM6 immunohistochemistry. We also set up Cox proportional hazard models for extensive associations between CpG methylation and survival. We identified loci highly correlated with cell growth and a targeted methylation signature of regulatory regions persistently associated with proliferation, grade, and survival. Candidate genes under the control of these regions include SMC4, ESRRG, PAX6, DOK7, VAV2, OTX1, and PCDHA-PCDHB-PCDHG, i.e., the protocadherin gene clusters. This study highlights the crucial role played by epigenetic mechanisms in shaping dysregulated cellular proliferation and provides potential biomarkers bearing prognostic and therapeutic value for the clinical management of meningioma.

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Section: Discussionmentioning

confidence: 99%

Correlation between DNA Methylation and Cell Proliferation Identifies New Candidate Predictive Markers in Meningioma

Hergalant

Saurel

Divoux

et al. 2022

Cancers

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“…Limited advancement has been achieved in the understanding of molecular mechanisms underlying the initiation and progression of BC [ 52–55 ]. Our study adds novel evidence for the oncogenic role of circLONP2 in supporting the malignant phenotype of BC, as well as its value as a prognostic indicator in BC patients.…”

Section: Discussionmentioning

confidence: 99%

Circular RNA LONP2 regulates proliferation, invasion, and apoptosis of bladder cancer cells by sponging microRNA-584-5p

et al. 2022

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Bladder cancer (BC) is the most frequent type of urinary tumor and a barely treatable disease. Although extensive efforts have been invested in the research of BC, the underlying etiology and pathophysiology remain unclear. CircLONP2 is a circular RNA implicated in the development of many cancers, and miR-584-5p and YAP1 have been reported to contribute to the progression of BC. In this research, we presented novel evidence supporting circLONP2/miR-584-5p/YAP1 axis as a novel regulatory module in the progression of BC. We analyzed the expression of circLONP2 between precancerous BC samples and normal tissues using a published RNA-seq dataset. The expression of circLONP2 was also validated in clinical samples and cell lines by quantitative RT-PCR. Small interfering RNA (siRNA) and miRNA inhibitor was utilized to modulate the expression of circLONP2 and miR-584-5p and investigate their functions on cell proliferation and invasion. Luciferase reporter assay and RNA pull-down were performed to confirm the functional interactions among circLONP2/miR-584-5p/YAP1. CircLONP2 was significantly upregulated in precancerous BC tissues and BC cells. CircLONP2 depletion inhibited cell viability, proliferation, and invasion of BC cell lines, which could be partially rescued by miR-584-5p inhibitor. Further experiments indicated that miR-584-5p regulates cell viability, proliferation, and invasion via directly targeting YAP1. In summary, our work indicates that circLONP2 plays an oncogenic function in BC by regulating miR-584-5p/YAP1 axis, and its interaction with miR-584-5p provides a potential strategy to target BC.

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“…Next, 100 U/mL ribonuclease A (RNase A; BD Biosciences) was added and mixed by shaking in a constant temperature water bath shaker for 30 min (37°C), followed by treatment with propidium iodide (PI; 10 μg/mL) staining solution containing Triton X-100, protected from light at 4°C for 30 min. The percentage of apoptotic cells was analyzed using the CellQuest software based on the appearance of subdiploid peaks (apoptotic peaks) on the DNA histogram [ 23 ].…”

Section: Methodsmentioning

confidence: 99%

Translocator protein (18 kDa) regulates the microglial phenotype in Parkinson’s disease through P47

et al. 2022

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Numerous studies have suggested that the phenotypic transformation of microglia plays a role in the pathogenesis of Parkinson’s disease (PD). Translocator protein (TSPO) is an 18 kDa translocator membrane protein that acts as a marker of neuroinflammation and suppresses neuroinflammation; however, its underlying mechanism remains unclear. Although TSPO ligands were found to be protective in several neurodegenerative paradigms, few studies have evaluated their effects on microglial polarization, and underlying mechanisms need to be explored. In the present study, we examined the effects of TSPO and PK11195, a TSPO ligand, on lipopolysaccharide (LPS)+interferon (IFN)-γ-induced inflammatory factors and oxidative stress in microglia using enzyme-linked immunosorbent assay. The effect of TSPO and PK11195 on LPS+IFN-γ-induced microglial cell apoptosis was examined using immunofluorescence (IF), flow cytometry, and western blotting. The interaction between TSPO and P47 was investigated using IF and co-immunoprecipitation analysis. In vivo experiments confirmed the influence of TSPO and its ligand on motility, a-Syn, and dopaminergic neuronal damage. Our findings indicate that TSPO may regulate the microglial phenotype in PD via P47, suggesting a potential role in anti-PD therapy.

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Orthodenticle homeobox OTX1 is a potential prognostic biomarker for bladder cancer

Cited by 15 publications

References 60 publications

Correlation between DNA Methylation and Cell Proliferation Identifies New Candidate Predictive Markers in Meningioma

Correlation between DNA Methylation and Cell Proliferation Identifies New Candidate Predictive Markers in Meningioma

Circular RNA LONP2 regulates proliferation, invasion, and apoptosis of bladder cancer cells by sponging microRNA-584-5p

Translocator protein (18 kDa) regulates the microglial phenotype in Parkinson’s disease through P47

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