Orthogonal Enzymatic Reactions to Control Supramolecular Hydrogelations

Abstract

Enzyme-responsive hydrogels have great potential in applications of controlled drug release, tissue engineering, etc. In this study, we reported on a supramolecular hydrogel that showed responses to two enzymes, phosphatase which was used to form the hydrogels and esterase which could trigger gel-sol phase transitions. The gelation process and visco-elasticity property of the resulting gel, morphology of the nanostructures in hydrogel, and peptide conformation in the self-assembled nanostructure were characterized by rheology, transmission electron microscope (TEM), and circular dichroism (CD), respectively. Potential application of the enzyme-responsive hydrogel in drug release was also demonstrated in this study. Though only one potential application of drug release was proved in this study, the responsive hydrogel system in this study might have potentials for the applications in fields of cell culture, controlled-drug release, etc.

Time-dependent gel to gel transformation of a peptide based supramolecular gelator

“…36,37 This reorganization can be initiated by the application of external stimuli like light, [38][39][40] pH 41,42 or enzymes. 43,44 In most cases gelto-sol or sol-to-gel transformation has been reported, 45 while gel-to-gel transformation is relatively rare in this regard. [46][47][48] Thus, it will be interesting to synthesize and study a new gelator molecule that can exhibit observable gel-to-gel transformation through the naked eyes.…”

Crystal structure of bis[(5-amino-1H-1,2,4-triazol-3-yl-κN4)acetato-κO]diaquanickel(II) dihydrate

“…The anions of ATAA coordinate the Ni II cation through the nitrogen atom N1 of the triazole ring and the oxygen atom O53 of the carboxylate group (Fig. 2), similarly to the complexes of ATCA with various metal cations (Chen et al, 2011;Sun et al, 2011;Wang et al, 2011;Herná ndez-Gil et al, 2012). The six-membered chelate ring adopts a slightly twisted boat conformation with puckering parameters of Q = 0.542 (2) Å , Â = 88.5 (2), ' = 15.4 (3) .…”

“…5-Amino-1H-1,2,4-triazole-3-carboxylic acid (ATCA, Fig. 1) was found to be a promising chelating ligand for which complexes with various metal cations have been reported recently (Chen et al, 2011;Sun et al, 2011;Wang et al, 2011;Herná ndez-Gil et al, 2012;Tseng et al, 2014). In these complexes, metal cations are ISSN 1600-5368…”

“…of the substituent in the position 3 of the triazole ring play the role of a donor atom, were found in the Cambridge Structural Database (Version 5.35, November 2013 with 2 updates; Thomas et al, 2010). The database reveals a total of seven structures of coordination compounds of 5-amino-1H-1,2,4triazole-3-carboxylic acid (ATCA) with various metals(Chen et al, 2011;Sun et al, 2011;Wang et al, 2011;Herná ndez-Gil et al, 2012;Tseng et al, 2014;Siddiqui et al, 2011), six of which are chelate complexes. Coordination compounds of metals with the ATAA ligands or its derivatives were not found in the literature.…”

A Synthetic Amino Acid Residue Containing A New Oligopeptide‐Based Photosensitive Fluorescent Organogel

“…Stimuli-responsive gels are particularly important owing to their various potential applications. [6h, 11] There are different stimuli-responsive gels in the literature including photosensitive, [12] pH-responsive, [5b, 13] enzyme-responsive, [14] and redox-dependent gels. [15] For the past few years functional organogels containing chromophores have drawn significant attention as these soft materials have found immense interest as novel materials for optoelectronic and other applications.…”