“Orthograde entrance technique” to recover DNA from ancient teeth preserving the physical structure

Alakoç, Yeşim Doğan; Aka, P. Sema

doi:10.1016/j.forsciint.2009.03.020

Cited by 25 publications

(16 citation statements)

References 17 publications

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“…The majority of ancient and forensic research using human teeth has focused on different ways to access the internal tooth tissue dentine (Alakoc and Aka, 2009;Cobb, 2002;Gilbert et al, 2003;Merriwether et al,1994;Shiroma et al, 2004), and has not considered the potential variation in mtDNA content between the different tissues and regions of the tooth (Fig. 1).…”

Section: Introductionmentioning

confidence: 99%

“…However, the physical methods used to powder skeletal remains, has the potential to influence the quantity and quality of recoverable DNA, although this has not been properly investigated. The two main powdering techniques involve directly drilling the sample with a dental burr or drill bit (Alakoc and Aka, 2009;Cobb, 2002;Gilbert et al, 2003;Merriwether et al, 1994;Shiroma et al, 2004), or pulverization with a shaking bead in a bone mill (Meyer et al, 2000;Ramos et al, 1995;Sampietro et al, 2006). Drilling is often preferred where there is a requirement for the specimen's morphological integrity to be maintained (e.g.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Survival and recovery of DNA from ancient teeth and bones

Adler

Haak

Donlon

et al. 2011

Journal of Archaeological Science

171

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Survival and recovery of DNA from ancient teeth and bones

Adler

Haak

Donlon

et al. 2011

Journal of Archaeological Science

171

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“…Decontamination procedure which was applied in the present study, did not include any bleaching agents or detergents, in contrast to that were applied in the previous studies (Sweet & Hildebrand, 1998;Gaytmenn & Sweet, 2003b;Kemp & Smith, 2005;Marjanovic et al, 2007;Alakoc & Aka, 2009). (Higgins et al, 2013), proved that bleaching agents decreased the amount of DNA yield in comparison to non-bleach treated teeth.…”

Section: Discussionmentioning

confidence: 63%

Sex determination from dental pulp DNA among Egyptians

Kholief

El-Shanawany

Gomaa

2017

Egypt J Forensic Sci

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Background: A great challenge arises when sex determination of human remains is needed, especially when only, bone fragments or isolated teeth are available; such as in mass disasters. Thus, the aim of the present work is to extract and analyze the DNA from dental pulp for sex determination of a sample of Egyptian population using the polymerase chain reaction amplification of DYS14 and SRY genes. Materials and methods: DNA was extracted from the dental pulp of forty freshly extracted teeth (sound and carious) collected from adult Egyptians of both sexes using QIAamp® DNA Investigator Kit. Androgen Receptor gene was PCR amplified as an internal positive control. DYS14 and SRY genes were PCR amplified for sex determination. Results: DNA extraction was confirmed by detection of the amplified AR gene band (292 bp) in all samples. Sex was successfully determined for all the studied teeth as revealed by amplification of 158 bp band, 778 bp band of the DYS14 and SRY genes respectively. Conclusion: DYS14 and SRY genes were found to be reliable for dental sex determination regardless of the condition of teeth, whether sound or carious.

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“…The technique also requires the skills of a dentist to prepare the endodontic access in the most appropriate area of the dental surface and to drill the pulp canal. The minimum dimension of the chamber access not only conserves in great part the tooth, but reduces the risk of external contamination during and after the pulp extraction, as documented by Alakoç and Aka [13].…”

Section: Discussionmentioning

confidence: 96%