Orthophosphate and alkaline phosphatase induced the formation of apatite with different multilayered structures and mineralization balance

Du, Tianming; Niu, Yumiao; Jia, Zhenzhen; Liu, Youjun; Qiao, Aike; Yang, Haisheng; Niu, Xufeng

doi:10.1039/d1nr06016c

Cited by 14 publications

(10 citation statements)

References 54 publications

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“…ACP and HA crystals have different calcium (Ca): phosphorus (P) ratio ratios and crystallinity [ 6 ]. Therefore, it is an effective mechanical factor due to its higher stiffness and crystallinity [ 7 , 8 , 9 , 10 ]. HA can effectively promote the growth of osteoblast with high crystallinity.…”

Section: Introductionmentioning

confidence: 99%

Physical and Chemical Characterization of Biomineralized Collagen with Different Microstructures

Niu

Liu

et al. 2022

JFB

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Mineralized collagen is the basic unit in hierarchically organized natural bone with different structures. Polyacrylic acid (PAA) and periodic fluid shear stress (FSS) are the most common chemical and physical means to induce intrafibrillar mineralization. In the present study, non-mineralized collagen, extrafibrillar mineralized (EM) collagen, intrafibrillar mineralized (IM) collagen, and hierarchical intrafibrillar mineralized (HIM) collagen induced by PAA and FSS were prepared, respectively. The physical and chemical properties of these mineralized collagens with different microstructures were systematically investigated afterwards. Transmission electron microscopy (TEM) and scanning electron microscopy (SEM) showed that mineralized collagen with different microstructures was prepared successfully. The pore density of the mineralized collagen scaffold is higher under the action of periodic FSS. Fourier transform infrared spectroscopy (FTIR) analysis showed the formation of the hydroxyapatite (HA) crystal. A significant improvement in the pore density, hydrophilicity, enzymatic stability, and thermal stability of the mineralized collagen indicated that the IM collagen under the action of periodic FSS was beneficial for maintaining collagen activity. HIM collagen fibers, which are prepared under the co-action of periodic FSS and sodium tripolyphosphate (TPP), may pave the way for new bone substitute material applications.

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Section: Introductionmentioning

confidence: 99%

Physical and Chemical Characterization of Biomineralized Collagen with Different Microstructures

Niu

Liu

et al. 2022

JFB

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“…After the water washout treatment (Figure d1), the amount of reticular filament structure on the surface of the screen decreased, which may be attributed the fact that some loose fibers that were not tightly bonded on the surface were washed away (Figure d2). In future studies, in order to enhance the strength and toughness of gel, mineralized gel is worth trying. − Moreover, the underwater OCA after the quartz sand wear test and water flow impact test were 163.7° and 166.4°, respectively (Figure c2,d2, inset), indicating that excellent superoleophobic performance still remained. The PAM–SA double network coated on the screen could also be well maintained, and the performance of wear resistance and stability in a water environment was also excellent.…”

Section: Results and Discussionmentioning

confidence: 99%

Stainless Steel Screen Modified with Renatured Xerogel for Efficient and Highly Stable Oil/Water Separation via Gravity

Yang

Shu

et al. 2023

Langmuir

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The application of hydrogel coatings to surfacemodified metallic materials has gained considerable attention in engineering practice such as water−oil separation. However, the low coating adhesion and poor coating stability restrict its application. In this study, to obtain special wettability and durable filter materials, polyacrylamide (PAM)/sodium alginate (SA) xerogel particles were first prepared and adhered to a stainless steel screen by using an epoxy resin as a linker. Subsequently, the xerogel particles of the screen rehydrates in water to form a PAM− SA double-network hydrogel. The results show that the screen modified by PAM−SA xerogel of 20−30 μm particle size and a linker concentration of 0.1 g/mL resulted in a chimeric structure and subsequently transformed a uniform double-network hydrogel coating in water. According to the experimental results, the rough hydrogel coating exhibits superhydrophilicity and superoleophobicity under water; in particular, it has excellent wear resistance as well as physical and chemical stability. Under gravity-driven action, the PAM−SA-modified screen demonstrates high separation efficiency values of up to 99% in separating a wide range of oil/water mixtures and maintaining a water flux of (2−6) × 10 4 L•m −2 • h −1 . There was no significant reduction in efficiency of separation and water flux after 10 cycles, indicating that the PAM−SAmodified screen is capable of offering outstanding separation performance and durability. Moreover, the hydrogel-modified screen demonstrated corrosion and swelling resistance in some extreme environments, paving a way for practical applications in water treatment. The novel hydrogel-coating-modified screen with ease of preparation holds great promise for oil/water separation and other engineering applications.

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“…In addition to the degradation and alkaline microenvironment, the mineral ion also plays considerable roles in the osteogenic differentiation during cell culture [45]. The rapid release of Ca and P ions in the IMHCS also helped to promote the osteogenic differentiation (figure 8(iii)), which was specifically expressed that the gene expression level related to the osteogenic differentiation of BMSCs on the IMHCS was significantly higher than on the HAS and CS [46].…”

Section: Discussionmentioning

confidence: 99%

In-situ mineralized homogeneous collagen-based scaffolds for potential guided bone regeneration

Du¹,

Gao²,

Tang³

et al. 2022

Biofabrication

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For guided bone regeneration (GBR) in clinical orthopedics, the importance of a suitable scaffold which can provide the space needed for bone regeneration and simultaneously promotes the new bone formation cannot be overemphasized. Due to its excellent biocompatibility, mechanical strength, and similarity in structure and composition to natural bone, the mineralized collagen-based scaffolds have been increasingly considered as promising GBR scaffolds. Herein, we propose a novel method to fabricate an in-situ mineralized homogeneous collagen-based scaffold (IMHCS) with excellent osteogenic capability for GBR by electrospinning the collagen solution in combination with essential mineral ions. The IMHCS exhibited homogeneous distribution of apatite crystals in electrospun fibers, which helped to achieve a significantly higher tensile strength than the pure collagen scaffold (CS) and the scaffold with directly added nano-hydroxyapatite particles (HAS). Furthermore, the IMHCS had significantly better cell compatibility, cell migration ratio, and osteogenic differentiation property than the HAS and CS. Therefore, the IMHCS not only retains traditional function of inhibiting fibroblast invasion, but also possesses excellent osteogenic differentiation property, indicating a robust alternative for GBR applications.

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Orthophosphate and alkaline phosphatase induced the formation of apatite with different multilayered structures and mineralization balance

Abstract: Mineralized collagen is a natural organic-inorganic composite. The combination of organic collagen and inorganic apatite to form different nanostructures is the key to produce bone- substitutes with biomechanical properties that...

Cited by 14 publications

References 54 publications

Physical and Chemical Characterization of Biomineralized Collagen with Different Microstructures

Physical and Chemical Characterization of Biomineralized Collagen with Different Microstructures

Stainless Steel Screen Modified with Renatured Xerogel for Efficient and Highly Stable Oil/Water Separation via Gravity

In-situ mineralized homogeneous collagen-based scaffolds for potential guided bone regeneration

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