Orthotopic models of cancer for preclinical drug evaluation

Bibby, M. C.

doi:10.1016/j.ejca.2003.11.021

Cited by 292 publications

(226 citation statements)

References 27 publications

Supporting

Mentioning

216

Contrasting

Unclassified

Order By: Relevance

“…All animals injected with LS174T tumor cells subcutaneously developed tumors. Tumors became palpable at Ϸ9 days after injection and were measured up to Ϸ17 days after injection, where the tumor reached Ϸ1000 mm 3 . No animal in either cohort dropped below 85% of its starting weight at any timepoint, nor did any increase dramatically with tumor onset.…”

Section: Adverse Effectsmentioning

confidence: 99%

“…2c,d), which is most likely due to an underestimation of the mean value at this timepoint caused by fewer animal numbers. However, considering the animals individually, the maximum tumor volume reached was 5500 mm 3 and the estimated mean doubling time (Td) was Ϸ2-3 days. In contrast, subcutaneous LS174T xenografts became palpable at 6 -7 days postinoculation, reaching a mean tumor volume of 700 mm 3 17 days after inoculation.…”

Section: Metastatic Progression Assessed By Susceptibility Contrast Mrimentioning

confidence: 99%

“…The majority of in vivo preclinical tumor model systems used in cancer research and drug development utilize solid tumors formed from either cell suspensions or tissue pieces implanted subcutaneously into the flanks of rodents (3). While these models are reproducible and easy to monitor, they do not represent all aspects of the microenvironment of the primary or metastatic sites of the tumor of origin (4).…”

mentioning

confidence: 99%

“…While these models are reproducible and easy to monitor, they do not represent all aspects of the microenvironment of the primary or metastatic sites of the tumor of origin (4). As a result, the phenotype of subcutaneous xenograft models can differ markedly from human tumors (3,5). Orthotopic transplantation of human tumor material into the appropriate anatomical site may more closely mimic human tumor growth (3,4).…”

mentioning

confidence: 99%

“…As a result, the phenotype of subcutaneous xenograft models can differ markedly from human tumors (3,5). Orthotopic transplantation of human tumor material into the appropriate anatomical site may more closely mimic human tumor growth (3,4).…”

mentioning

confidence: 99%

See 4 more Smart Citations

Longitudinal in vivo susceptibility contrast MRI measurements of LS174T colorectal liver metastasis in nude mice

Kalber

Waterton

Griffiths

et al. 2008

Magnetic Resonance Imaging

View full text Add to dashboard Cite

Purpose: To characterize longitudinal tumor progression in a murine orthotopic model of liver metastasis using susceptibility contrast magnetic resonance imaging (MRI). Materials and Methods:Nude mice were inoculated intrasplenically with LS174T colorectal carcinoma cells 24 hours postadministration of 2.5 mgFe/kg of the ultrasmall superparamagnetic iron oxide particle preparation feruglose. Contiguous T 2 and T 2 *-weighted multislice MR images were acquired 10, 15, 20, 25, 30, and 35 days postinoculation to longitudinally evaluate metastatic progression. Functional tumor vasculature and hypoxia were histologically evaluated at the final timepoint using Hoechst 33342 uptake, pimonidazole and hematoxylin and eosin staining. A parallel cohort of subcutaneous tumors was included for comparison.Results: All intrasplenically inoculated mice developed liver metastases, evident in both T 2 *-and T 2 -weighted images as high-signal deposits, compared to feruglose-nulled normal liver. Small lesions were detected as early as day 10 and all mice exhibited progressing lesions over 35 days. Liver metastases took longer to establish, but exhibited a similar volume doubling time to the subcutaneously propagated tumors of Ϸ2-3 days. Different functional tumor vascular architectures between the two growth sites were apparent.Conclusion: Susceptibility-contrast MRI using a single dose of feruglose can be used to easily detect and longitudinally monitor orthotopically propagated liver metastases in vivo.

show abstract

Section: Adverse Effectsmentioning

confidence: 99%

Section: Metastatic Progression Assessed By Susceptibility Contrast Mrimentioning

confidence: 99%

mentioning

confidence: 99%

mentioning

confidence: 99%

mentioning

confidence: 99%

See 3 more Smart Citations

Longitudinal in vivo susceptibility contrast MRI measurements of LS174T colorectal liver metastasis in nude mice

Kalber

Waterton

Griffiths

et al. 2008

Magnetic Resonance Imaging

View full text Add to dashboard Cite

show abstract

Oncolytic Vaccinia Virus Therapy of Salivary Gland Carcinoma

Chernichenko¹,

Linkov²,

Li³

et al. 2013

JAMA Otolaryngol Head Neck Surg

View full text Add to dashboard Cite

Objective To examine the therapeutic effects of an attenuated, replication-competent vaccinia virus (GLV-1h68) against a panel of 5 human salivary gland carcinoma cell lines. Design The susceptibility of 5 salivary gland carcinoma cell lines to infection and oncolysis by GLV-1h68 was assessed in vitro and in vivo. Results All 5 cell lines were susceptible to viral infection, transgene expression, and cytotoxic reactions. Three cell lines were exquisitely sensitive to infection by very low doses of GLV-1h68. Orthotopic parotid tumors exhibited more aggressive behavior compared with flank tumors. A single intratumoral injection of GLV-1h68 induced significant tumor regression without observed toxic effects in flank and parotid tumor models; controls demonstrated rapid tumor progression. Conclusion These promising results demonstrate significant oncolytic activity by an attenuated vaccinia virus for infecting and lysing salivary gland carcinomas, supporting future clinical trials.

show abstract

Mouse Models in Cancer Research

Lee¹,

Schmitt²

2006

Apoptosis and Cancer Therapy

View full text Add to dashboard Cite

Orthotopic models of cancer for preclinical drug evaluation

Cited by 292 publications

References 27 publications

Longitudinal in vivo susceptibility contrast MRI measurements of LS174T colorectal liver metastasis in nude mice

Longitudinal in vivo susceptibility contrast MRI measurements of LS174T colorectal liver metastasis in nude mice

Oncolytic Vaccinia Virus Therapy of Salivary Gland Carcinoma

Mouse Models in Cancer Research

Contact Info

Product

Resources

About