Oryeongsan (Wulingsan) ameliorates impaired ANP secretion of atria from spontaneously hypertensive rats

Amelioration of Hypertension by Oryeongsan through Improvements of Body Fluid and Sodium Balance: Roles of the Renin-Angiotensin System and Atrial Natriuretic Peptide System

“…Previously, it was shown that modulation of the intracardial expression of the AT 1 receptor and AT 2 receptor expression was associated with an accentuation of the secretion of ANP in the atria from hypertensive rats [ 23 , 48 , 49 ]. Furthermore, a recent study shows that chronic treatment with ORS accentuated the suppressed ANP secretion in the perfused beating atria from spontaneously hypertensive rats along with a decrease in abundance of the AT 1 receptor and increase of AT 2 receptor and Mas receptor expression [ 50 ]. It is worthwhile to comment on the role of accentuation of the atrial synthesis and release of ANP in the regulation of body sodium and water homeostasis in hypertension [ 51 ].…”

Herbal medicine (Oryeongsan) for fluid and sodium balance in renal cortex of spontaneously hypertensive rats

“…These notion supports the difference between ORS (present study) and Compound-21 14 in the control of the RAS signaling pathways, AT 2 R-NHE3 signaling pathway in the renal proximal tubules from SHR in particular. Significance of the present study is that the treatment with ORS ameliorates hypertension through broad action mechanisms: modulation of renal excretory function (present study), atrial ANP secretion, 3 and vascular responsibility (present study) in the SHR. Further, it is an important finding that ORS suppresses expression of the AT1R-NHE3 signaling pathway in the kidney, because NHE3 expression is upregulated in the proximal tubule or the medulla of the kidney.…”

“…The mechanisms involved in the decrease in SBP of SHR by treatment with ORS could be three-fold: one is correction by ORS of the impaired Na + and water balance, and the other is amelioration of the impaired vasodilation and then accentuation of ANP secretion from the atria. 3 Chronic treatment with ORS decreased NHE3 expression concomitantly with an accentuation of AT 2 receptor and suppression of AT 1 receptor in the renal cortex leading to a decrease in Na + and water retention in the SHR-ORS group. Regulation of the basal blood pressure and the development of the high blood pressure are closely associated with the regulation of the urinary flow and excretion of Na + and body Na + and water balance through the renal function.…”

“…ORS water extract was prepared as previously reported. 3 Five medicinal herbs ( Alisma orientale Juzepzuk, 9.375 g; Poria cocos Wolf, 5.625 g; Atractylodes macrocephala Koidzumi, 5.625 g; Polyporus umbellatus Fries, 5.625 g; Cinnamomum cassia Presl, 1.875 g; total weight to be 28.1 g of mixture of the dried herbs of ORS, a single dose for a human adult) were mixed. Mixture of the dried herbs of ORS (3.0 kg) was extracted in distilled water at 100 °C for 2 h using an electric extractor.…”

“… 2 Recently, it was further shown that chronic treatment with ORS accentuated atrial natriuretic peptide (ANP) secretion in the atria from spontaneously hypertensive rats (SHR) in which the hormone secretion was suppressed. 3 The ORS accentuation for the ANP secretion in the atria from SHR was accompanied by suppression of angiotensin (Ang) I converting enzyme (ACE)-Ang II type 1 (AT 1 ) receptor signaling and accentuation of AT 2 receptor/ACE2-Mas receptor signaling pathway expression in the atria. These findings are in relation with the notion that the herbal formula ORS may promote the cardio-renal function leading to volume and pressure homeostasis via modulation of the renin-angiotensin system (RAS) and natriuretic peptide system (NPS).…”