Oryza sativa protein phosphatase 1a (OsPP1a) involved in salt stress tolerance in transgenic rice

Liao, Yu-Duan; Lin, Kuan–Hung; Chen, Chiu-Chen; Chiang, Chih-Ming

doi:10.1007/s11032-016-0446-2

Cited by 55 publications

(32 citation statements)

References 65 publications

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“…In rice, RSS1 (RICE SALT SENSITIVE 1) protein was reported to interact with OsPP1a, most likely to control meristem maintenance upon salt stress [ 12 , 22 ] and overexpression of OsPP1a confers salinity tolerance to rice plants [ 23 ]. Deciphering the pleiotropic roles of PP1 in plants is difficult because of the high diversity of PIPs driving a wide range of substrates and because of the increased number of PP1 isoforms that are redundant in subcellular location and expression pattern.…”

Section: Introductionmentioning

confidence: 99%

Genome wide identification of wheat and Brachypodium type one protein phosphatases and functional characterization of durum wheat TdPP1a

et al. 2018

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Reversible phosphorylation is an essential mechanism regulating signal transduction during development and environmental stress responses. An important number of dephosphorylation events in the cell are catalyzed by type one protein phosphatases (PP1), which catalytic activity is driven by the binding of regulatory proteins that control their substrate specificity or subcellular localization. Plants harbor several PP1 isoforms accounting for large functional redundancies. While animal PP1s were reported to play relevant roles in controlling multiple cellular processes, plant orthologs remain poorly studied. To decipher the role of plant PP1s, we compared PP1 genes from three monocot species, Brachypodium, common wheat and rice at the genomic and transcriptomic levels. To gain more insight into the wheat PP1 proteins, we identified and characterized TdPP1a, the first wheat type one protein phosphatase from a Tunisian durum wheat variety Oum Rabiaa3. TdPP1a is highly conserved in sequence and structure when compared to mammalian, yeast and other plant PP1s. We demonstrate that TdPP1a is an active, metallo-dependent phosphatase in vitro and is able to interact with AtI2, a typical regulator of PP1 functions. Also, TdPP1a is capable to complement the heat stress sensitivity of the yeast mutant indicating that TdPP1a is functional also in vivo. Moreover, transient expression of TdPP1a::GFP in tobacco leaves revealed that it is ubiquitously distributed within the cell, with a strong accumulation in the nucleus. Finally, transcriptional analyses showed similar expression levels in roots and leaves of durum wheat seedlings. Interestingly, the expression in leaves is significantly induced following salinity stress, suggesting a potential role of TdPP1a in wheat salt stress response.

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Section: Introductionmentioning

confidence: 99%

Genome wide identification of wheat and Brachypodium type one protein phosphatases and functional characterization of durum wheat TdPP1a

et al. 2018

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“…Several studies reported that overexpression of stress-induced/transcription factor/transporter genes from rice or other plant species conferred salinity tolerance in rice. Some of these genes conferring enhanced salt tolerance in rice are OsLEA4 8 , OsPP1a 9 , OsGly1 10 , OsJRL 11 . Although transgenic plants targeting the modification of a single gene have shown improved tolerance to salinity, progress in this area of research is hampered due to lack of complete understanding of the molecular basis of salt tolerance, which involves a complex network of genes operating in close coordination 12 , 13 .…”

Section: Introductionmentioning

confidence: 99%

Activation-tagging in indica rice identifies a novel transcription factor subunit, NF-YC13 associated with salt tolerance

Manimaran

Reddy

Moin

et al. 2017

Sci Rep

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Nuclear factor Y (NF-Y) is a heterotrimeric transcription factor with three distinct NF-YA, NF-YB and NF-YC subunits. It plays important roles in plant growth, development and stress responses. We have reported earlier on development of gain-of-function mutants in an indica rice cultivar, BPT-5204. Now, we screened 927 seeds from 70 Ac/Ds plants for salinity tolerance and identified one activation-tagged salt tolerant DS plant (DS-16, T3 generation) that showed enhanced expression of a novel ‘histone-like transcription factor’ belonging to rice NF-Y subfamily C and was named as OsNF-YC13. Localization studies using GFP-fusion showed that the protein is localized to nucleus and cytoplasm. Real time expression analysis confirmed upregulation of transcript levels of OsNF-YC13 during salt treatment in a tissue specific manner. Biochemical and physiological characterization of the DS-16 revealed enhanced K+/Na+ ratio, proline content, chlorophyll content, enzymes with antioxidant activity etc. DS-16 also showed transcriptional up-regulation of genes that are involved in salinity tolerance. In-silico analysis of OsNF-YC13 promoter region evidenced the presence of various key stress-responsive cis-regulatory elements. OsNF-YC13 subunit alone does not appear to have the capacity for direct transcription activation, but appears to interact with the B- subunits in the process of transactivation.

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“…They are usually considered as unexciting enzymes as they are involved in the turning off of the signaling cascades triggered by protein kinases (País et al, 2009). But, recently it has been documented that it positively regulate the salt stress responsive pathways (Liao et al, 2016). In a latest research, overexpression of protein phosphatase 1a (OsPP1a) has been reported to enhance salinity tolerance in rice (Liao et al, 2016).…”

Section: Regulatory Proteinsmentioning

confidence: 99%

“…But, recently it has been documented that it positively regulate the salt stress responsive pathways (Liao et al, 2016). In a latest research, overexpression of protein phosphatase 1a (OsPP1a) has been reported to enhance salinity tolerance in rice (Liao et al, 2016). In an another study, a rice phosphatase 2C has been found to confer salt tolerance in Arabidopsis (Singh et al, 2015).…”

Section: Regulatory Proteinsmentioning

confidence: 99%

Integrating Classical with Emerging Concepts for Better Understanding of Salinity Stress Tolerance Mechanisms in Rice

Kaur

Pati

2017

Front. Environ. Sci.

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Rice is an important cereal crop responsible for world's food security. The sensitivity of rice plants toward a range of abiotic stresses is a prime challenge for its overall growth and productivity. Among these, salinity is a major stress which results in a significant loss of global rice yield annually. For finding straightforward and strict future solutions in order to assure the food security to growing world population, understanding of the various mechanisms responsible for salt stress tolerance in rice is of paramount importance. In classical studies, identification of salt tolerant cultivars and the genetic markers linked to salt tolerance and breeding approaches have been given emphasis. It further affirmed on the identification of various pathways regulating the complex process of salt stress adaptation. However, only limited success has been achieved in these approaches as salt tolerance is a complex process and is governed by multiple factors. Hence, for better understanding of salt tolerance mechanisms, a comprehensive approach involving physiological, biochemical and molecular studies is much warranted. Modern experimental and genetic resources have provided a momentum in this direction and have provided molecular insights into different salt stress responsive pathways at the signaling and regulatory level. The integrative knowledge of classical and modern research of the understanding of salt stress adaptive pathways can help the researchers for designing effective strategies to fight against salt stress. Hence, the present review is focused on the understanding of the salt stress tolerance mechanisms in rice through the consolidative knowledge of classical and modern concepts. It further highlights the emerging new trends of salt stress adaptive pathways in rice.

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Oryza sativa protein phosphatase 1a (OsPP1a) involved in salt stress tolerance in transgenic rice

Cited by 55 publications

References 65 publications

Genome wide identification of wheat and Brachypodium type one protein phosphatases and functional characterization of durum wheat TdPP1a

Genome wide identification of wheat and Brachypodium type one protein phosphatases and functional characterization of durum wheat TdPP1a

Activation-tagging in indica rice identifies a novel transcription factor subunit, NF-YC13 associated with salt tolerance

Integrating Classical with Emerging Concepts for Better Understanding of Salinity Stress Tolerance Mechanisms in Rice

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